Comparative Proteomic Analysis of the Effect of the Four-Herb Chinese Medicine ANBP on Promoting Mouse Skin Wound Healing

Abstract

Traditional Chinese medicine has great potential to improve wound healing. ANBP, the mixture of 4 Chinese herbs- Agrimoniapilosa, Nelumbonucifera, Boswelliacarteri, and Pollen typhae-is effective in trauma treatment while its mechanism is still elusive. In this study, quantitative proteomics and bioinformatics analyses were performed to decipher the possible roles of ANBP in accelerated wound healing of mouse skin. Among all 3171 identified proteins, 90, 71, 80, and 140 proteins were found to be differently expressed in 6 hours, 3 days, 7 days, and 14 days ANBP-treated tissues compared with corresponding control tissues, respectively. The result showed that different biological processes and pathways were activated at different healing stages. At the early healing stage, ANBP treatment mainly affected several biological processes, including immune and defense response, vascular system restoration, hemostasis and coagulation regulation, lipid metabolism and signal transduction, while muscle tissue, hair, epidermis, extracellular matrix and tissue remodeling related activities were the major events in ANBP promoted later wound healing. This is the first quantitative proteome study of ANBP-treated wound tissues, which provide a new perspective for the mechanism of ANBP accelerated wound healing and is of guiding significance for clinical application of ANBP in trauma disorders cure.

Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats

“…This technique is known for its high throughput, high sensitivity and high precision, and thoroughly and quantitatively analyzes the genre, number and correlation of proteins, by which complicated network and possible regulatory mechanisms are investigated[ 19 - 21 ]. Protein microarray has been gradually applied to research into traditional Chinese medicine (TCM), such as pharmacodynamics and toxicology of Chinese medications[ 22 - 24 ], yet it has rarely been used in the study of acupuncture-moxibustion therapy.…”

Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis

“…The healing proteome has been studied in different animal models, especially in mice and pigs. These studies showed that proteins with differential expression correspond to receptors, hydrolases, enzymes, immunity, cytoskeletal proteins, transporters, and oxidoreductases (Chen et al, 2017; Sabino et al, 2015). Additionally, in pathogenic processes such as type 2 diabetes and chronic ulcers, where there is an increase in expression levels of proteins related to inflammation and tissue destruction, proteins with differential expression have been identified, most of which relate to angiogenesis, cell death, and inflammation (Eming et al, 2010; Krisp et al, 2013).…”

Peer Review #2 of "Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis (v0.1)"

“…These studies showed that proteins with differential expression correspond to receptors, hydrolases, enzymes, immunity, cytoskeletal proteins, transporters and oxidoreductases (28,29).…”