Comparative proteomic analysis of seedling leaves of cold-tolerant and -sensitive spring soybean cultivars

Abstract

Cold stress adversely affects the growth and development of seedling of spring soybean. Revealing responses in seedling to cold stress at proteomic level will help us to breed cold-tolerant spring soybean cultivars. In this study, to understand the responses, a proteomic analysis on the leaves of seedlings of one cold-tolerant soybean cultivar and one cold-sensitive soybean cultivar at 5°C for different times (12 and 24 h) was performed, with some proteomic results being further validated by physiological and biochemical analysis. Our results showed that 57 protein spots were found to be significantly changed in abundance and identified by MALDI-TOF/TOF MS. All the identified proteins were found to be involved in 13 metabolic pathways and cellular processes, including photosynthesis, protein folding and assembly, cell rescue and defense, cytoskeletal proteins, transcription and translation regulation, amino acid and nitrogen metabolism, protein degradation, storage proteins, signal transduction, carbohydrate metabolism, lipid metabolism, energy metabolism, and unknown. Based on the majority of the identified cold-responsive proteins, the effect of cold stress on seedling leaves of the two spring soybean cultivars was discussed. The reason that soybean cv. Guliqing is more cold-tolerant than soybean cv. Nannong 513 was due to its more protein, lipid and polyamine biosynthesis, more effective sulfur-containing metabolite recycling, and higher photosynthetic rate, as well as less ROS production and lower protein proteolysis and energy depletion under cold stress. Such a result will provide more insights into cold stress responses and for further dissection of cold tolerance mechanisms in spring soybean.

Screening and Expression Analysis of Temperature-responsing Proteins that Can Remove Low Temperature Stagnancy of Seeds Germination of <i>Lepidium apetalum</i>

“…17 cm precast linear IPG strips (pH3-10) were chosen for the Isoelectric Focusing, sample volume was unified according to the quality of seed. Methods refer to 2-DE process of spring soybean (Glycine max L.) (Tian et al, 2015) and wild wheat (Triticum urartu L.) (Gharechahi et al, 2014), the methods were changed slightly in this study. Compared with the first and second group, screening differentially expressed proteins in the third group of seeds which removed the low-temperature germination stagnancy.…”

Deciphering soybean molecular stress response via high-throughput approach

“…Constant progress in the available technologies ena-Table 1 . Summary of the described studies of soybean stress response analyzed with high-throughput technologies focused on transcriptomic, miRNA and proteomic analyses Transcriptome studies miRNA studies Proteome studies Drought Le et al, 2012Chen et al, 2013Shin et al, 2015Tripathi et al, 2016Li et al, 2011-Flooding Nanjo et al, 2011aNanjo et al, 2010Nanjo et al, 2011bYin et al, 2014aKhan et al, 2015Yin & Komatsu, 2015 Cold stress -- Tian et al, 2015Salinity -Dong et al, 2013Sun et al, 2016Ma et al, 2012 Phosphate deficiency Zeng et al, 2015Wang et al 2016Xu et al, 2013Zenga et al, 2010Chen et al, 2011 Biotic stress Lanubile et al, 2015Li et al, 2012bYin et al, 2013 -High-throughput analyses of soybean stress response bles us to create gene expression atlases, which may present snapshots of the transcriptome profiles even for the whole life-cycles of plants. Moreover, expression profiling obtained by using high-throughput methods contributes to the development of molecular markers, finding genes responsible for the secondary metabolism and studying the evolution of organs in plant families.…”

“…Among 41 soybean landraces and cultivars of south China, two were chosen: Guliqing (cold-tolerant) and Nannong 513 (cold-sensitive) to analyze and describe the cold stress responses at a proteomic level (Tian et al, 2015). The cultivars were incubated at 5°C for 12 and 24 h. It has been shown that 57 protein spots on 2DE gels, isolated from the first trifoliate leaves, were found to be significantly altered in abundance.…”

iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings

“…In recent years, quantitative proteomic analysis has been applied to investigate the response to abiotic stress in plants [16][17][18]. Proteomic analysis of plants under heat stress has been widely performed, and some heat-responsive proteins and metabolic pathways have been identi ed to play important roles in the process of plant response to heat stress [19,20].…”