Comparative proteomic analysis of rat left ventricle in a subtotal nephrectomy model

Lin, Yang; Yu, Wen Chung; Hsu, Meng Erh; Tsai, Hsiao Chien; Liao, Chen Chung; Chao, Lin

doi:10.1016/j.jcma.2014.08.016

Cited by 4 publications

(1 citation statement)

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“…Some researchers have found that chronic renal failure of the 5/6 nephrectomy rats were associated with mitochondrial damage 15 . Proteomic studies of rat cardiomyopathy showed that CKD cardiomyopathy was involved in mitochondrial dysfunction, oxidative phosphorylation and other pathways 16 . Mitochondria are often the primary source of reactive oxygen species (ROS), and oxidative phosphorylation is a process known to produce ROS.…”

Section: Discussionmentioning

confidence: 99%

Changes of protein levels in human urine reflect the dysregulation of signaling pathways of chronic kidney disease and its complications

Hao

Reyes

Morris

et al. 2020

Sci Rep

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The increasing prevalence of chronic kidney disease (CKD) seriously is threatening human health and overall quality of life. The discovery of biomarkers of pathogenesis of CKD and the associated complications are very important for CDK diagnosis and treatment. In this paper, urine protein biomarkers were investigated because urine sample collection is convenient and non-invasive. We analyzed the protein concentrations in the urine of CKD patients and extracted abnormal protein signals comparing with the healthy control groups. The enriched signaling pathways that may characterize CKD pathology were identified from these proteins. We applied surface-enhanced laser desorption and ionization time of flight mass spectrometry technology to detect different protein peaks in urine samples from patients with CKD and healthy controls. We searched the proteins corresponding to protein peaks through the UniProt database and identified the signaling pathways of CKD and its complications by using the NIH DAVID database. 42 low abundance proteins and 46 high abundance proteins in the urine samples from CKD patients were found by comparing with healthy controls. Seven KEGG pathways related to CKD and its complications were identified from the regulated proteins. These pathways included chemokine signaling pathway, cytokine–cytokine receptor interaction, oxidative phosphorylation, cardiac muscle contraction, Alzheimer’s disease, Parkinson's disease, and salivary secretion. In CKD stages 2, 3, 4, and 5, five proteins showed significantly differential abundances. The differential protein signals and regulated signaling pathways will provide new insight for the pathogenesis of CKD and its complications. These altered proteins may also be used as novel biomarkers for the noninvasive and convenient diagnosis methods of CKD and its complications through urine testing in the future.

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Section: Discussionmentioning

confidence: 99%