Comparative proteomic analysis of pituitary glands from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach

L, Xin; Cao, Zhenzhen; Xing, Zhe; Liu, Mei; Gao, Ming; Meng, Bo; Fan, Ruiming

doi:10.1371/journal.pone.0185253

Cited by 9 publications

(7 citation statements)

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“…As in our previous report, the expression of ITPR3, a member of the family of inositol 1,4,5‐trisphosphate (IP 3 ) receptors, was found to be down‐regulated in hypothalamus tissue from Huoyan geese during the laying period (Luan et al., ). IP 3 is an intracellular second messenger that transduces neurotransmitter signals.…”

Section: Discussionmentioning

confidence: 52%

Comparative proteomic analysis of hypothalamus tissue from Huoyan geese between pre‐laying period and laying period using an iTRAQ‐based approach

Cao

Fan

Meng

et al. 2018

Animal Science Journal

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The hypothalamus plays a central role in controlling poultry endocrine and reproductive activities. So far there is limited information focused on the proteome profiles of the hypothalamus from geese during different stages of the egg-laying cycle. In order to identify proteins regulating the egg-laying process of Huoyan geese, we investigated the proteome profiles of the hypothalamus from Huoyan geese during the laying period and pre-laying period by applying an isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic technology. A total number of 3,337 were identified and quantified, of which 18 were significantly up-regulated and 16 were significantly down-regulated. These differentially expressed proteins were subjected to bioinformatics analyses based on the Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway. Some of these were revealed to be involved in hormone and neurotransmitter secretion, exocytosis, calcium ion transport and synaptic transmission. Subsequently, excitatory amino acid transporter 2, complexin-1 and inositol 1,4,5-trisphosphate receptor, type 3 were confirmed at the messenger RNA level using quantitative real-time RT-PCR. Then, the abundance change of these proteins was verified further using Western blotting analysis. These data may aid in elucidating the molecular mechanism of higher laying performance in Huoyan geese.

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Section: Discussionmentioning

confidence: 52%

Comparative proteomic analysis of hypothalamus tissue from Huoyan geese between pre‐laying period and laying period using an iTRAQ‐based approach

Cao

Fan

Meng

et al. 2018

Animal Science Journal

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“…iTRAQ is one of the most advanced technology in modern quantitative proteomics [21, 22]; it combines stable isotope labeling with tandem mass spectrometry [23–25] to compare the relative amount of proteins from normal and diseased samples in a single experiment. Wang WS et al [26] revealed that myoferlin is a novel prognostic predictor in pancreatic adenocarcinoma through iTRAQ-based quantitative proteomics.…”

Section: Discussionmentioning

confidence: 99%

ITRAQ-based proteomic analysis reveals possible target-related proteins in human adrenocortical adenomas

et al. 2019

BMC Genomics

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Background Adrenocortical adenomas (ACAs) can lead to the autonomous secretion of aldosterone responsible for primary aldosteronism (PA), which is the most common form of secondary arterial hypertension. However, the authentic fundamental mechanisms underlying ACAs remain unclear. Objective Isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics and bioinformatics analyses from etiological studies of ACAs were performed to screen the differentially expressed proteins (DEPs) and investigate the relevant mechanisms of their occurrence and development. Results could help determine therapeutic targets of clinical significance. Methods In the present study, iTRAQ-based proteomics was applied to analyze ACA tissue samples from normal adrenal cortex tissues adjacent to the tumor. Using proteins extracted from a panel of four pairs of ACA samples, we identified some upregulated proteins and other downregulated proteins in all four pairs of ACA samples compared with adjacent normal tissue. Subsequently, we predicted protein–protein interaction networks of three DEPs to determine the authentic functional factors in ACA. Results A total of 753 DEPs were identified, including 347 upregulated and 406 downregulated proteins. The expression of three upregulated proteins (E2F3, KRT6A, and ALDH1A2) was validated by Western blot in 24 ACA samples. Our data suggested that some DEPs might be important hallmarks during the development of ACA. Conclusions This study is the first proteomic research to investigate alterations in protein levels and affected pathways in ACA using the iTRAQ technique. Thus, this study not only provides a comprehensive dataset on overall protein changes but also sheds light on its potential molecular mechanism in human ACAs. Electronic supplementary material The online version of this article (10.1186/s12864-019-6030-5) contains supplementary material, which is available to authorized users.

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“…Additionally, in this network, casein kinase 2 alpha 2 (CSNK2A2) is down-regulated in the pituitary of HEPH during the PS. CSNK2A2 encodes an uncharacterized protein in avian species but this protein was shown to be decreased in laying geese pituitaries when compared to non-laying geese, indicating a possible role in egg production or ovulation [17].…”

Section: The Hpg Axismentioning

confidence: 99%

Transcriptome analysis of the hypothalamus and pituitary of turkey hens with low and high egg production

Brady

Liu

Hicks

et al. 2020

Preprint

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Background: High egg producing hens (HEPH) show increased hypothalamic and pituitary gene expression related to hypothalamo-pituitary-gonadal (HPG) axis stimulation as well as increased in vitro responsiveness to gonadotropin releasing hormone (GnRH) stimulation in the pituitary when compared to low egg producing hens (LEPH). Transcriptome analysis was performed on hypothalamus and pituitary samples from LEPH and HEPH to identify novel regulators of HPG axis function. Results: In the hypothalamus and pituitary, 4644 differentially expressed genes (DEGs) were identified between LEPH and HEPH, with 2021 genes up-regulated in LEPH and 2623 genes up-regulated in HEPH. In LEPH, up-regulated genes showed enrichment of the hypothalamo-pituitary-thyroid (HPT) axis. Beta-estradiol was identified as an upstream regulator regardless of tissue. When LEPH and HEPH samples were compared, beta-estradiol was activated in HEPH in 3 of the 4 comparisons, which correlated to the number of beta-estradiol target genes up-regulated in HEPH. In in vitro pituitary cell cultures from LEPH and HEPH, thyroid hormone pretreatment negatively impacted gonadotropin subunit mRNA levels in cells from both LEPH and HEPH, with the effect being more prominent in HEPH cells. Additionally, the effect of estradiol pretreatment on gonadotropin subunit mRNA levels in HEPH cells was negative, whereas estradiol pretreatment increased gonadotropin subunit mRNA levels in LEPH cells.Conclusions: Up-regulation of the HPT axis in LEPH and upstream beta-estradiol activation in HEPH may play a role in regulating HPG axis function, and ultimately ovulation rates. Furthermore, thyroid hormone and estradiol pretreatment impacted gonadotropin mRNA levels following GnRH stimulation, with the inhibitory effects of thyroid hormone being more detrimental in HEPH and estradiol stimulatory effects being more prominent in LEPH. Differential responsiveness to thyroid hormone and estradiol pretreatment may be due to desensitization of target genes to thyroid hormone and estradiol in LEPH and HEPH, respectively, in response general up-regulation of the HPT axis in LEPH and of the HPG axis in HEPH. Further studies will be necessary to identify possible target gene desensitization mechanisms and elicit the full role that the HPT axis and beta-estradiol upstream regulation play in egg production rates in turkey hens.

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Comparative proteomic analysis of pituitary glands from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach

Cited by 9 publications

References 69 publications

Comparative proteomic analysis of hypothalamus tissue from Huoyan geese between pre‐laying period and laying period using an iTRAQ‐based approach

Comparative proteomic analysis of hypothalamus tissue from Huoyan geese between pre‐laying period and laying period using an iTRAQ‐based approach

ITRAQ-based proteomic analysis reveals possible target-related proteins in human adrenocortical adenomas

Transcriptome analysis of the hypothalamus and pituitary of turkey hens with low and high egg production

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