Comparative proteomic analysis of mesenchymal stem cells derived from human bone marrow, umbilical cord, and placenta: Implication in the migration

Abstract

Umbilical cord (UC) and placenta (P) have been suggested as alternatives to bone marrow (BM) as sources of mesenchymal stem cells (MSC) for cell therapy, with both UC- and P-MSC possess immunophenotypic and functional characteristics similar to BM-MSC. However, their migration capacity, which is indispensable during tissue regeneration process, is unclear. Under defined conditions, the migration capacity of BM- and P-MSC was found 5.9- and 3.2-folds higher than that of UC-MSC, respectively. By the use of 2-DE and combined MS and MS/MS analysis, six differentially expressed proteins were identified among these MSC samples, with five of them known to be involved in cell migration as migration enhancing or inhibiting proteins. Consistent with their migration capacity, the levels of migration enhancing proteins including cathepsin B, cathepsin D and prohibitin,were significantly lower in UC-MSC when compared with those in BM- and P-MSC. For the migration inhibiting proteins such as plasminogen activator inhibitor-1 (PAI-1) and manganese superoxide dismutase, higher expression was found in the UC-MSC. We also showed that the overexpression of the PAI-1 impaired the migration capacity of BM- and P-MSC while silencing of PAI-1 enhanced the migration capacity of UC-MSC. Our study indicates that PAI-1 and other migration-related proteins are pivotal in governing the migration capacity of MSC.

Umbilical cord fibroblasts: Could they be considered as mesenchymal stem cells?

“…Furthermore, the above-cited studies raise the necessity to update the criteria that de- [1] CTSB, CTSD, PHB [10] , BSP [7] , Leptin [12] No report for osteo-, adipo or chondrogenic differentiation failure SSEA-4, LNGFR [6] , CD146…”

“…Proteomic is an excellent tool to study and compare expressed protein profile of MSCs. 2D gel analysis revealed that BM-MSCs highly express proteins involved in cell migration (CTSB, CTSD and PHB), which correlates with their important migration potential [10] . These migration-enhancing proteins were minimally expressed in UC-MSC, which expressed migration inhibitory proteins (PAI-1 and MnSOD).…”

Proteomic profile of visceral adipose tissues between low-fat diet-fed obesity-resistant and obesity-prone C57BL/6 mice

“…Protein extraction of adipose tissues, ieF and second dimensional separation of samples, gel staining and image analysis, trypticin-gel digestion, Maldi-ToF/ToF mass spectrometric analysis and database search for protein identification were carried out according to previously described methods (13). Briefly, the cell pellets were re-suspended in lysis buffer (8 M urea, 2 M thiourea, 2% CHaPS, 1% nP-40, 2 mM TBP, 1X Protease inhibitor Mix, 1X nuclease Mix, 1 mM PMSF and 2% iPG buffer) and held on ice for 45 min.…”

Differential expression of cell surface proteins in human bone marrow mesenchymal stem cells cultured with or without basic fibroblast growth factor containing medium

“…After this report, proteomic approaches have been applied to identify their key regulatory molecules and potential biomarkers. Proteomic analyses of whole MSCs derived from a number of different human tissues including bone marrow, umbilical cord blood, placenta, and adipose tissues have been conducted using 2-DE [46,47]. In the other approaches, 2-DE has been used in efforts to increase the understanding of the MSC differentiation process into specific cell types such as neurons [48], adipocytes [49,50], and osteoblasts [51].…”