Comparative proteomic analysis of head kidney among three Antarctic fishes

Jia, Ruonan; Huang, Shaojun; Zhai, Wanying; Jiang, Shouwen; Li, Wenhao; Wang, Faxiang; Xu, Qianghua

doi:10.1007/s00227-022-04043-w

Cited by 1 publication

(1 citation statement)

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“…Several toll-like receptor genes within the Notothenioid clade were shown to be under positive selection in pathogen recognition domains suggesting adaptation to the specific Antarctic microbiota [41]. A proteome study of the head kidney between white blood fish, Chionodraco hamatus, and two red blood fish, Trematomus bernacchii and N. coriiceps, revealed differences in erythropoiesis, heme biogenesis, leucocyte, and platelet development, with upregulation of lymphoid and megakaryocytic lineage marker proteins in ice fish [45].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Down-Regulation of Immune System Components in Barrier and Hematopoietic Tissues after Lipopolysaccharide Injection in Antarctic Notothenia coriiceps

Sousa

Power

Guerreiro

et al. 2022

Fishes

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The environmental conditions and isolation in the Antarctic have driven the evolution of a unique biodiversity at a macro to microorganism scale. Here, we investigated the possible adaptation of the teleost Notothenia coriiceps immune system to the cold environment and unique microbial community of the Southern Ocean. The fish immune system was stimulated through an intraperitoneal injection of lipopolysaccharide (LPS 0111:B4 from E. coli) and the tissue transcriptomic response and plasma biochemistry were analyzed 7 days later and compared to a sham injected control. Gene transcription in the head-kidney, intestine and skin was significantly modified by LPS, although tissues showed different responsiveness, with the duodenum most modified and the skin the least modified. The most modified processes in head-kidney, duodenum and skin were related to cell metabolism (up-regulated) and the immune system (comprising 30% of differentially expressed genes). The immune processes identified were mostly down-regulated, particularly interleukins and pattern recognition receptors (PRRs), nucleotide-binding oligomerization domain-like receptors and mannose receptors, unlike the toll-like receptors response commonly described in other teleost fish. The modified transcriptional response was not mirrored by a modified systemic response, as the circulating levels of enzymes of innate immunity, lysozyme and antiproteases, were not significantly different from the untreated and sham control fish. In conclusion, while the N. coriiceps immune system shares many features with other teleosts there are also some specificities. Further studies should better characterize the PRRs and their role in Antarctic teleosts, as well as the importance of the LPS source and its consequences for immune activation in teleosts.

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Section: Introductionmentioning

confidence: 99%