Comparative Proteomic Analysis of Differentially Expressed Proteins in Shoots of Salicornia europaea under Different Salinity

Abstract

Soil salinity is a major abiotic stress that limits agriculture productivity worldwide. Salicornia europaea is a succulent annual euhalophyte and one of the most salt tolerant plant species. The elucidation of its salt tolerance mechanism is of significance for generating salt-tolerant crops. In this study, we provided high resolution of proteome reference maps of S. europaea shoot and obtained evidence on the salt tolerance mechanism by analyzing the proteomic responses of this plant to high salinity. Our results demonstrated significant variations existed in 196 out of 1880 protein spots detected on CBB stained 2-DE gels. Of these, 111 proteins were identified by mass spectrometry. Among them, the majority was energy production and conversion related proteins, followed by photosynthesis and carbohydrate metabolism associated enzymes. Analysis of protein expression patters revealed that energy production and ion homeostasis associated proteins played important roles for this plant salt tolerance ability. Hierarchical clustering results revealed many proteins were involved in S. europaea salt tolerance mechanism as a dynamic network. Finally, based on our proteomic results, we brought forward a possible schematic representation of mechanism associated with the systematic salt tolerance phenotype in S. europaea.

Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance

“…The strips were then placed into an Ettan IPGphor isoelectric focusing system, and isoelectric focusing was performed following the manufacturer's instructions (GE Healthcare, Uppsala, Sweden). The proteins were separated in the second dimension as described elsewhere (20). Each 2-DE separation was repeated at least three times for each sample to ensure reproducibility of the protein patterns.…”

“…An improved borax/polyvinylpolypyrrolidone/phenol (BPP) protocol for isolating total proteins from halophytes was developed by us for the proteomic analysis of the true halophyte Salicornia europaea (19). By removing interfering compounds and salt ions (19), the BPP method enabled a comparative proteomic analysis of S. europaea grown under different levels of salinity (20). Subsequently, we further compared the technical details in Coomassie Brilliant Blue staining and developed the more sensitive protocol GAP, which uses Coomassie Brilliant Blue G-250, ammonium sulfate, and phosphoric acid (21).…”

Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress

“…1,2 Salinization is rapidly increasing on a global scale and currently effects more than 10% of arable land resulting in greater than 50% decline in the average yields of major crops. 3 Salt stress induces oxidative stress through the over production of reactive oxygen species (ROS) that trigger lipid peroxidation, damage to photosynthetic pigments and disturbance in mineral nutrient status. 4,5 The inhibitory effect of salt stress on photosynthetic rate may involve stomatal or non-stomatal limitations and inhibition of photochemical processes 6 by disturbing the water balance in plants, homeostasis of Na C and Cl ¡ ions and nutrient uptake.…”

Proteomics Approach for Identifying Salt Stress Related Proteins in Barley Leaves

“…Moreover, Heat shock protein 70 (spot 2) was up-regulated under salt stress. Previous studies have shown that the levels of HSP70 proteins were increased in Salicornia europaea under salt stress (Wang et al, 2009). These results indicate that members in the HSP family might have diverse functions in plant salt tolerance.…”