Comparative Proteomic Analysis of <i>Helicobacter pylori</i> Strains Isolated from Chinese Patients

Abstract

Helicobacter pylori, the major cause of gastritis, peptic ulcer and gastric cancer, infects half of the world population, but only a few infections lead to serious disease. In order to investigate specific proteins related to the pathogenic difference of this bacterium, comparative proteome analyses of Helicobacter pylori C1 (isolated from patients with gastric cancer) and G1 (isolated from patients with gastritis) were performed using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Four proteins (inorganic pyrophosphatase, 3-oxoadipate CoA-transferase subunit B, translation elongation factor, and aldo-keto reductase) were found only in Helicobacter pylori C1, and one protein (alkyl hydroperoxide reductase) was found in G1. Additionally, different isoelectric points (pI) of Hsp60 were observed from the two strains. Then we cloned and sequenced Hsp60 genes from forty-nine Helicobacter pylori isolated from gastric cancer and gastritis. Gene sequencing showed that one C→G single nucleotide polymorphism occurred in the 1399th nucleotide of Hsp60. These results indicate that pathogenic differences exist in various Helicobacter pylori isolated from Chinese patients.