Comparative proteomic analyses provide novel insights into the effects of grafting wound and hetero-grafting per se on bottle gourd

Wang, Lingping; Li, Guojing; Wu, Xiaohua; Xu, Pei

doi:10.1016/j.scienta.2015.12.056

Cited by 7 publications

(7 citation statements)

References 35 publications

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“…Therefore, characterization of the physiological and molecular mechanisms involved in graft responses at early stages of development was in focus of several studies in stone fruit trees (Irisarri et al, 2016;Reig et al, 2019) and grapevines (Cookson et al, 2014;Assunção et al, 2019). Differentially expressed transcripts, proteins, and secondary metabolites accumulated at the graft interface were reported in several studies to improve our understanding of the molecular-level differences between heterografts and homografts (Wang et al, 2016., Chen et al, 2017Pina et al, 2017;Gautier et al, 2019;Prodhomme et al, 2019). However, the advancement of graft incompatibility (GI) studies was slow compared to other agronomic traits such as resistance to biotic and abiotic stresses, self-fertility, or fruit quality, because it is logistically challenging to graft hundreds of different scion-rootstock combinations with a sufficient number of replicates to quantify the trait.…”

Section: Introductionmentioning

confidence: 99%

Mapping Quantitative Trait Loci Associated With Graft (In)Compatibility in Apricot (Prunus armeniaca L.)

et al. 2021

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Graft incompatibility (GI) between the most popular Prunus rootstocks and apricot cultivars is one of the major problems for rootstock usage and improvement. Failure in producing long-leaving healthy grafts greatly affects the range of available Prunus rootstocks for apricot cultivation. Despite recent advances related to the molecular mechanisms of a graft-union formation between rootstock and scion, information on genetic control of this trait in woody plants is essentially missing because of a lack of hybrid crosses, segregating for the trait. In this study, we have employed the next-generation sequencing technology to generate the single-nucleotide polymorphism (SNP) markers and construct parental linkage maps for an apricot F1 population “Moniqui (Mo)” × “Paviot (Pa)” segregating for ability to form successful grafts with universal Prunus rootstock “Marianna 2624”. To localize genomic regions associated with this trait, we genotyped 138 individuals from the “Mo × Pa” cross and constructed medium-saturated genetic maps. The female “Mo” and male “Pa” maps were composed of 557 and 501 SNPs and organized in eight linkage groups that covered 780.2 and 690.4 cM of genetic distance, respectively. Parental maps were aligned to the Prunus persica v2.0 genome and revealed a high colinearity with the Prunus reference map. Two-year phenotypic data for characters associated with unsuccessful grafting such as necrotic line (NL), bark and wood discontinuities (BD and WD), and an overall estimate of graft (in)compatibility (GI) were collected for mapping quantitative trait loci (QTLs) on both parental maps. On the map of the graft-compatible parent “Pa”, two genomic regions on LG5 (44.9–60.8 cM) and LG8 (33.2–39.2 cM) were associated with graft (in)compatibility characters at different significance level, depending on phenotypic dataset. Of these, the LG8 QTL interval was most consistent between the years and supported by two significant and two putative QTLs. To our best knowledge, this is the first report on QTLs for graft (in)compatibility in woody plants. Results of this work will provide a valuable genomic resource for apricot breeding programs and facilitate future efforts focused on candidate genes discovery for graft (in)compatibility in apricot and other Prunus species.

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Section: Introductionmentioning

confidence: 99%

Mapping Quantitative Trait Loci Associated With Graft (In)Compatibility in Apricot (Prunus armeniaca L.)

et al. 2021

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“…The most complete proteome analysis done to date was done in homo-grafts of bottle gourd at the graft interface and in the scion just above the graft interface 7 DAG along with un-grafted controls using mass spectroscopy-based techniques (Wang et al, 2016 ). The graft interface was associated with the accumulation of proteins related to wound responses and defense signaling such as genes from the gene ontology (GO) groups “response to stimulus,” “phenol-containing compound metabolic process,” “oxidoreduction coenzyme metabolic process,” and “salicylic acid metabolic process.” The proteins accumulated in the scion relative to the un-grafted control were enriched in the GO terms “response to abiotic stimulus,” suggesting that grafting alters protein abundance beyond the graft interface.…”

Section: Identifying the Transcripts And Proteins Associated With Tismentioning

confidence: 99%

“…Comparative proteomic analysis of hetero- vs. homo-grafting has been done on bottle gourd 7 DAG and the hetero-graft interface was associated with the accumulation of proteins related to hydrogen peroxide (Wang et al, 2016 ). Other proteome studies (using gel electrophoresis-based techniques) on an in vitro callus graft system in Prunus spp .…”

Section: Identifying Transcripts and Proteins Associated With Graft Umentioning

confidence: 99%

“…Plant have complex antioxidant system to control ROS via non-enzymatic (such as carotenoids, tocopherols, flavonoids, ascorbate, glutathione and proline) and enzymatic antioxidants [such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), dehydroascorbate reductase (DHR), and ascorbate peroxidase (APX)]. The high expression of genes (Cookson et al, 2013 ; Assunção et al, 2019b ; Xie et al, 2019 ) and the accumulation of proteins (Wang et al, 2016 ; Xu et al, 2017 ) associated with different elements of the antioxidant system is frequently observed at the graft interface in homo-grafts. Some studies have measured the activity of certain antioxidant enzymes during homo-graft formation, but frequently not at the graft interface itself or without adequate intact plants or stem control samples (Fernandez-Garcia et al, 2004 ; Miao et al, 2019 ).…”

Section: Identifying Redox Markers Of Grafting Success And/or Graft Imentioning

confidence: 99%

“…The idea that hetero-grafting together different genotypes results in an increased level of oxidative stress at the graft interface has been supported by gene expression (Cookson et al, 2014 ; Assunção et al, 2019b ) and proteomic studies (Wang et al, 2016 ; Xu et al, 2017 ). Some studies have measured the activity of antioxidant enzymes at the graft interface of different scion/rootstock combinations.…”

Section: Identifying Redox Markers Of Grafting Success And/or Graft Imentioning

confidence: 99%

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Identifying Molecular Markers of Successful Graft Union Formation and Compatibility

Loupit

Cookson

2020

Front. Plant Sci.

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Grafting is a technique used for millennia for vegetative propagation, especially in perennial fruit crops. This method, used on woody and herbaceous plants, can improve several agronomic characteristics, such as yield or vigor, as well as tolerance to biotic and abiotic stresses. However, some scion/rootstock combinations suffer from poor graft compatibility, i.e., they are unable to form and/or sustain a successful graft union. Identifying symptoms of graft incompatibility is difficult because they are not always present in the first years after grafting and in most cases the causes of incompatibility are still poorly understood. Studies of changes in transcript abundance during graft union formation indicate that grafting responses are similar to responses to wounding and include the differential expression of genes related to hormone signaling, oxidative stress, formation of new vascular vessels, cell development, and secondary metabolites, in particular polyphenols. This review summarizes current knowledge of the changes in transcript abundance, redox status and metabolites accumulation during graft union formation and in cases of graft incompatibility. The goal of this review is to discuss the possibility of identifying marker transcripts, enzyme activities and/or metabolites of grafting success and graft compatibility which could be used to score grafting success for genetic research and in breeding programs. We highlight gaps in current knowledge and potential research directions in this field.

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Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

et al. 2023

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Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species, hampering clonal forestry programs. This study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile, peroxidase activity, macro- and micronutrient contents, and adventitious rooting of mini cuttings. The analyses were performed on E. microcorys shoots which were successfully obtained by seminal and grafting propagation, micropropagation with epicormic shoots, and indirect organogenesis. Among four mature trees used in the propagation, tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or reinvigoration. Five individuals from each technique were randomly selected and transferred to a semi-hydroponic “channel” system. After four weeks in the seedbed, the total protein, peroxidase activity, nutrient content and rooting of the mini cuttings were evaluated. SDS-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques, as revealed by two distinct bands. Materials obtained by micropropagation with epicormic shoots showed the highest peroxidase activity, while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity. A portable X-ray fluorescence spectroscope (pXRF) identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation, and by indirect organogenesis. The analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation (75%) followed by indirect organogenesis (35%). Based on principal component analysis, it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis was associated with phosphorous, sulphur, and potassium contents, which suggests a higher level of tissue rejuvenation/reinvigoration in these propagated plants. Further studies are recommended to search for other methods that present similarities with the responses to adventitious rooting in forest species and thus optimize the rescue and propagation of plants with distinct ontogenetic stages.

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Comparative proteomic analyses provide novel insights into the effects of grafting wound and hetero-grafting per se on bottle gourd

Cited by 7 publications

References 35 publications

Mapping Quantitative Trait Loci Associated With Graft (In)Compatibility in Apricot (Prunus armeniaca L.)

Mapping Quantitative Trait Loci Associated With Graft (In)Compatibility in Apricot (Prunus armeniaca L.)

Identifying Molecular Markers of Successful Graft Union Formation and Compatibility

Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

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