Comparative proteomic analyses of Asian cotton ovules with attached fibers in the early stages of fiber elongation process

Abstract

BackgroundPlenty of proteomic studies were performed to characterize the allotetraploid upland cotton fiber elongation process, whereas little is known about the elongating diploid cotton fiber proteome.MethodsIn this study, we used a two-dimensional electrophoresis-based comparative proteomic approach to profile dynamic proteomes of diploid Asian cotton ovules with attached fibers in the early stages of fiber elongation process. One-way ANOVA and Student-Newman-Keuls test were used to find the differentially displayed protein (DDP) spots.ResultsA total of 55 protein spots were found having different abundance ranging from 1 to 9 days post-anthesis (DPA) in a two-day interval. These 55 DDP spots were all successfully identified using high-resolution mass spectrometric analyses. Gene ontology analyses revealed that proteoforms involved in energy/carbohydrate metabolism, redox homeostasis, and protein metabolism are the most abundant. In addition, orthologues of the 13 DDP spots were also found in differential proteome of allotetraploid elongating cotton fibers, suggesting their possible essential roles in fiber elongation process.ConclusionsOur results not only revealed the dynamic proteome change of diploid Asian cotton fiber and ovule during early stages of fiber elongation process but also provided valuable resource for future studies on the molecular mechanism how the polyploidization improves the trait of fiber length.Electronic supplementary materialThe online version of this article (doi:10.1186/s12953-016-0101-1) contains supplementary material, which is available to authorized users.

Proteomics of Cotton Fiber Development

“…Further, these spots were identified using high-resolution mass spectrometry and gene ontology analyses. These differential proteome expressions mainly involved in the carbohydrate/ energy metabolism and redox homeostasis (Zhang et al, 2016).…”

Proteomic Profiles of Cotton Fiber Developmental Transition from Cell Elongation to Secondary Wall Deposition

“…fiber developmental transition stage, 14-3-3 protein 6-like, fasciclin-like arabinogalactan protein 1 and STS14 protein-like were only downregulated in Gh, whereas another key signal transduction related molecule, Ras-related protein RABA1f-like, was downregulated in Gb. Moreover, a sulfurtransferase is reported to be abundant in the plant cell wall and plasma membrane, promoting cell differentiation[35]. In Gh cotton fibers, the upregulation of sulfurtransferase as well as guanosine nucleotide diphosphate dissociation inhibitor (GDI) was recorded.…”