Comparative proteome analysis of Picrorhiza kurrooa Royle ex benth. in response to drought

Sanjeeta,; Parkash, Jai; Kalita, Prakash Jyoti; Devi, M. Tirumala; Pathania, Jyoti; Joshi, Robin; Dutt, Som

doi:10.7243/2050-2273-3-2

Cited by 10 publications

(5 citation statements)

References 54 publications

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“…However, ribosomal protein S7 and translation initiation factor 1 expression decreases after 7 DWD up to 14 DWD (Table 3). Enhanced expressions of ribosomal proteins L36 were reported under drought stress[43]. Differential expression of ribosomal protein S4 was noted during Cd, NaCl and their combined treatment in S .…”

Section: Discussionmentioning

confidence: 99%

Drought mediated physiological and molecular changes in muskmelon (Cucumis melo L.)

et al. 2019

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Water deficiency up to a certain level and duration leads to a stress condition called drought. It is a multi-dimensional stress causing alteration in the physiological, morphological, biochemical, and molecular traits in plants resulting in improper plant growth and development. Drought is one of the major abiotic stresses responsible for loss of crops including muskmelon (Cucumis melo. L). Muskmelon genotype SC-15, which exhibits high drought resistance as reported in our earlier reports, was exposed to deficient water condition and studied for alteration in physiological, molecular and proteomic profile changes in the leaves. Drought stress results in reduced net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration (E) rate. With expanded severity of drought, declination recorded in content of total chlorophyll and carotenoid while enhancement observed in phenol content indicating generation of oxidative stress. In contrary, activities of catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and guaiacol (POD) were increased under drought stress. Peptide mass fingerprinting (PMF) showed that drought increased the relative abundance of 38 spots while decreases10 spots of protein. The identified proteins belong to protein synthesis, photosynthesis, nucleotide biosynthesis, stress response, transcription regulation, metabolism, energy and DNA binding. A drought-induced MADS-box transcription factor was identified. The present findings indicate that under drought muskmelon elevates the abundance of defense proteins and suppresses catabolic proteins. The data obtained exhibits possible mechanisms adopted by muskmelon to counter the impacts of drought induced stress.

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Section: Discussionmentioning

confidence: 99%

Drought mediated physiological and molecular changes in muskmelon (Cucumis melo L.)

et al. 2019

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“…It reduced leaf protein content by 24 and 37% on a fresh and dry weight basis, respectively. Drought stress increased root protein content by 12% on fresh weight and decreased by 9% on dry weight basis [45].…”

Section: Yieldmentioning

confidence: 94%

“…The imposition of drought in Vitex trifolia L. increased chlorophyll fluorescence and impaired PSII activity [40]. Drought stress also caused a differential expression of proteins involved in metabolism, photosynthesis, transcription, protein synthesis, defense response, transport, signaling, and cytoskeleton development in P. kurrooa [45]. Thus, we can infer from diverse species that drought and high temperatures can cause reduced transpiration in the long run, which can cause increased canopy temperatures and CWSI.…”

Section: Canopy Temperature and Transpirationmentioning

confidence: 99%

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Climate Change and Its Impact on Crops: A Comprehensive Investigation for Sustainable Agriculture

et al. 2022

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Plants are a highly advanced kingdom of living organisms on the earth. They survive under all climatic and weather variabilities, including low and high temperature, rainfall, radiation, less nutrients, and high salinity. Even though they are adapted to various environmental factors, which are variable, the performance of a crop will be compensated under sub/supra optimal conditions. Hence, current and future climate change factors pose a challenge to sustainable agriculture. Photosynthesis is the primary biochemical trait of crops that are affected by abiotic stress and elevated CO2 (eCO2). Under eCO2, the C3 legumes could perform better photosynthesis over C4 grasses. The associated elevated temperature promotes the survival of the C4 crop (maize) over C3 plants. In the American Ginseng, the elevated temperature promotes the accumulation of phytocompounds. Under less water availability, poor transpirational cooling, higher canopy temperatures, and oxidative stress will attenuate the stability of the membrane. Altering the membrane composition to safeguard fluidity is a major tolerance mechanism. For protection and survival under individual or multiple stresses, plants try to undergo high photorespiration and dark respiration, for instance, in wheat and peas. The redox status of plants should be maintained for ROS homeostasis and, thereby, plant survival. The production of antioxidants and secondary metabolites may keep a check on the content of oxidating molecules. Several adaptations, such as deeper rooting, epicuticular wax formation such as peas, and utilization of non-structural carbohydrates, i.e., wheat, help in survival. In addition to yield, quality is a major attribute abridged or augmented by climate change. The nutrient content of cereals, pulses, and vegetables is reduced by eCO2; in aniseed and Valeriana sp., the essential oil content is increased. Thus, climate change has perplexing effects in a species-dependent manner, posing hurdles in sustainable crop production. The review covers various scientific issues interlinked with challenges of food/nutritional security and the resilience of plants to climate variability. This article also glimpses through the research gaps present in the studies about the physiological effects of climate change on various crops.

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“…These were transplanted in plastic pots and maintained in the experimental farm of the institute as described previously (Gangola et al, 2013;Parkash et al, 2014a,b;Sanjeeta et al, 2014).…”

Section: Plant Materialsmentioning

confidence: 99%

Cathepsin B cysteine protease gene is upregulated during leaf senescence and exhibits differential expression behavior in response to phytohormones in Picrorhiza kurrooa Royle ex Benth.

et al. 2015

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Medicinal importance of Picrorhiza (Picrorhiza kurrooa Royle ex Benth -an herb of western Himalayan region) and its endangered status in Red Data Book presses an urgent need for intensive R&D interventions towards ensuring its availability for the medicinal use, its sustainability and improvement. The present study was conducted on cathepsin B cysteine protease in Picrorhiza. Cathepsin B cysteine protease has been reported to function in diverse processes such as senescence, abscission, programmed cell death, fruit ripening and in response to pathogen and pest attacks. A full-length cDNA-Pk-cbcp encoding cathepsin B-like cysteine protease was cloned from Picrorhiza. The full length Pk-cbcp cDNA consisted of 1369 bp with an open reading frame of 1080 bp, 80 bp 5′ untranslated region and 209 bp 3′ untranslated region. The deduced Pk-cbcp protein contained 359 amino acids with a molecular weight of 39.981 kDa and an isoelectric point of 5.75. Secondary structure analysis revealed that Pk-cbcp had 28.97% α-helices, 14.48% β-turns, 19.50% extended strands and 37.05% random coils. Semi-quantitative PCR analysis revealed 157% higher expression of Pk-cbcp during senescence compared to that of pre-senescence. Further, application of phytohormones abscisic acid, jasmonic acid and cytokinin influenced the temporal expression status of Pk-cbcp. Abscisic acid and jasmonic acid increased the expression level whereas cytokinin reduced the expression. The findings suggest the role of Pk-cbcp in leaf senescence in Picrorhiza which may be differentially mediated through phytohormones.

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Comparative proteome analysis of Picrorhiza kurrooa Royle ex benth. in response to drought

Cited by 10 publications

References 54 publications

Drought mediated physiological and molecular changes in muskmelon (Cucumis melo L.)

Drought mediated physiological and molecular changes in muskmelon (Cucumis melo L.)

Climate Change and Its Impact on Crops: A Comprehensive Investigation for Sustainable Agriculture

Cathepsin B cysteine protease gene is upregulated during leaf senescence and exhibits differential expression behavior in response to phytohormones in Picrorhiza kurrooa Royle ex Benth.

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