Comparative proteome analysis of high and low cadmium accumulating soybeans under cadmium stress

Abstract

A comparative proteomic study was performed to unravel the protein networks involved in cadmium stress response in soybean. Ten-day-old seedlings of contrasting cadmium accumulating soybean cultivars-Harosoy (high cadmium accumulator), Fukuyutaka (low cadmium accumulator), and their recombinant inbred line CDH-80 (high cadmium accumulator) were exposed to 100 μM CdCl(2) treatment for 3 days. Root growth was found to be affected under cadmium stress in all. Varietal differences at root protein level were evaluated. NADP-dependent alkenal double bond reductase P1 was found to be more abundant in low cadmium accumulating Fukuyutaka. Leaf proteome analysis revealed that differentially expressed proteins were primarily involved in metabolism and energy production. The results indicate that both high and low cadmium accumulating cultivars and CDH-80 share some common defense strategies to cope with the cadmium stress. High abundance of enzymes involved in glycolysis and TCA cycle might help cadmium challenged cells to produce more energy necessary to meet the high energy demand. Moreover, enhanced expressions of photosynthesis related proteins indicate quick utilization of photoassimilates in energy generation. Increased abundance of glutamine synthetase in all might be involved in phytochelatin mediated detoxification of cadmium ions. In addition, increased abundance of antioxidant enzymes, namely superoxide dismutase, ascorbate peroxidase, catalase, ensures cellular protection from reactive oxygen species mediated damages under cadmium stress. Enhanced expression of molecular chaperones in high cadmium accumulating cultivar might be another additional defense mechanism for refolding of misfolded proteins and to stabilize protein structure and function, thus maintain cellular homeostasis.

Influence of abiotic stresses on plant proteome and metabolome changes

“…The effect of aluminum and cadmium on antioxidant enzyme activities in two barley cultivars revealed that the activity of SOD was higher in the resistant genotype (Guo et al 2004). Among the cadmium-induced defense-related proteins between high-and low-cadmium-accumulating cultivars of soybean, the enhanced expressions of antioxidant enzymes, APX, CAT and Cu/Zn SOD, were evident in both genotypes (Hossain et al 2012). Analysis of the changes in the proteome of the roots of pea in response to salinity also revealed an increased accumulation of SOD, but no other antioxidant enzymes were identified (Kav et al 2004).…”

Proteomics of Important Food Crops in the Asia Oceania Region: Current Status and Future Perspectives

“…However, the growth, development, and yield of soybean are greatly affected by several abiotic stresses, such as flooding (reviewed by 160,161 ), drought (reviewed by 162 ), salinity (reviewed by 163 ), and heavy metal cadmium (reviewed by 164 ). The information gathered from recent proteomics research (Table 1) has covered all aspects of plant responses including seed germination 165 , the seedling stage [166][167][168] , the later stages of growth [169][170] , and the seed filling stage…”

“…Although soybean cultivars differ in their ability to take up, accumulate and translocate cadmium to aerial parts, little attention has been paid so far to unravel the underlying molecular mechanism of cadmium tolerance. To understand this mechanism, proteomic techniques have been used 167,179 . Ahsan et al 179 investigated differential responses of root microsomal proteins in contrasting cadmium accumulating soybean cultivars exposed to cadmium.…”

Heavy Metal Toxicity in Plants: Proteomics Approach

“…Bununla birlikte, Cr(VI) stresi altındaki bitkilerde azot metabolizması ile ilgili bir enzim olan nitrat redüktaz proteininin miktarında azalma belirlenmiştir (Sharmin et al 2012). Diğer taraftan, Cd stresine maruz bırakılan Arabidopsis thaliana ve soya fasulyesi bitkilerinde GS proteininin ekspresyonunda artış belirlenmiştir (Semane et al 2010;Hossain et al 2012a). GS proteininin ekspresyonundaki artış daha fazla GSH oluşumuna neden olabilmektedir.…”

“…Peroksiredoksinler gelişim, antioksidant, içsel apoptoz düzenleyicisi (Ichimia et al 1997), hücreler arası sinyal molekülü ve moleküler şaperon (Jang et al 2004) gibi farklı hücresel fonksiyonlara sahiptir. Bu proteinin ekspresyonundaki artış metal stresi altındaki birçok bitki türünde tespit edilmiştir (Labra et al 2006;Wang et al2011;Hossain et al 2012a). …”