Comparative protein accumulation patterns in soybean somatic and zygotic embryos

Dahmer, Mark L.; Hildebrand, David F.; Collins, G. B.

doi:10.1007/bf02823027

Cited by 29 publications

(21 citation statements)

References 44 publications

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“…The accumulation of protein at maturation stage is known to be crucial for high vigor and successful conversion of somatic embryos in legumes (21 ). In general, somatic embryos of legumes accumulate relatively low levels of proteins compared to seeds (20,8). In our procedure, the addition of 1g/l casein hydrolysate in Medium C promoted the development of high vigorous somatic embryos with 50% conversion rate in Vera cultivar.…”

Section: Resultsmentioning

confidence: 82%

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Barbulova¹,

Iancheva²,

Zhiponova³

et al. 2002

Biotechnology & Biotechnological Equipment

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Section: Resultsmentioning

confidence: 82%

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Barbulova¹,

Iancheva²,

Zhiponova³

et al. 2002

Biotechnology & Biotechnological Equipment

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“…Studies have reported that storage protein levels in somatic embryos of rapeseed [201,208], cotton [204], alfalfa [203,205], soybean [209], and white spruce [210] were lower than those of their zygotic counterparts, approximately 10% of the zygotic levels [201,205]. In addition, the developmental timing of storage protein accumulation has been reported to be abnormal [201,203,204], with storage proteins accumulating at earlier stages of development than in zygotic embryos.…”

Section: Storage Proteinsmentioning

confidence: 94%

“…The above mentioned studies used little or no ABA during somatic embryo maturation. Others, such as the abnormal reserve accumulation documented in soybean somatic embryos by Dahmer et al [209], may be the result ofembryo development in the presence ofrelatively high auxin levels. Due to the previously described beneficial effects of ABA on somatic embryo development, and known effects on zygotic embryo storage protein expression [213,214], the lack of sufficient ABA may account for the above results.…”

Section: Storage Proteinsmentioning

confidence: 97%

Morphogenic Aspects of Somatic Embryogenesis

Merkle¹,

Parrott²,

Flinn³

1995

In Vitro Embryogenesis in Plants

210

146

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“…Both b-conglycinin and glycinin were detected in developing embryos, with accumulation of the a and aЈ subunits of b-conglycinin beginning earlier than that of the b subunit of b-conglycinin and glycinin, as is the case in developing seeds ( Figure 3B). The levels of seed storage proteins in somatic embryos were previously found to be constant during maturation, with the b subunit of b-conglycinin (Dahmer et al 1992) or glycinin (Stejskal and Griga 1995) not being detected even in mature somatic embryos. However, in the present study, storage protein composition of somatic embryos and the amounts of these proteins changed during maturation.…”

Section: Temporal Regulation Of Storage Protein Expression During Sommentioning

confidence: 98%

Maturation of somatic embryos as a model for soybean seed development

Nishizawa

Ishimoto

2009

Plant Biotechnology

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Soybean somatic embryos have attracted attention both as a model of zygotic embryos and as explants for the generation of stable transgenic plants. We have now characterized the maturation of soybean somatic embryos in detail by examining both the accumulation of the major seed storage proteins b-conglycinin and glycinin as well as changes in cellular organization. Protein storage vacuoles and oil bodies, which are the main depositories of seed storage reserves, formed within cells during the maturation of somatic embryos. The seed storage proteins were gradually synthesized and accumulated in the protein storage vacuoles in a manner similar to that apparent in seeds. The a and a Ј subunits of bconglycinin were detected earlier than the b subunit of this protein and glycinin. In addition, The a and a Ј subunits of bconglycinin accumulated in both the cotyledons and the hypocotyl of somatic embryos, whereas the b subunit of bconglycinin and glycinin accumulated only in the cotyledons. These temporal and spatial characteristics of storage protein production in maturating somatic embryos are similar to those in developing seeds, although the maturation of somatic embryos ceases prematurely without attainment of the final stages of development. Our findings suggest that somatic embryos are suitable for verification of seed-specific traits such as the biosynthesis of seed components. Key words: Seed development, somatic embryogenesis, soybean (Glycine max [L.] Merr.), storage protein.Plant Biotechnology 26, 543-550 (2009) Original Paper a Present address: National Institute of Crop Science, Tsukuba, Ibaraki 305-8518, Japan. Abbreviations: BSA, bovine serum albumin; PAGE, polyacrylamide gel electrophoresis; PBS, phosphate-buffered saline; PCR, polymerase chain reaction; PSV, protein storage vacuole; RT, reverse transcription; a-TIP, a-tonoplast intrinsic protein. This article can be found at http://www.jspcmb.jp/ Mcmullen 1991; Khalafalla et al. 2005). Transgenic immature somatic embryos can also be maintained in culture, and homogeneous masses of such embryos can be readily and repeatedly subjected to induction of differentiation and maturation. Somatic embryos have therefore been used to screen for transgene expression and to examine transgene effects before recovery of whole plants (Cahoon et al. 1999;Cahoon et al. 2000;Cahoon et al. 2002;Herman et al. 2003;Chen et al. 2006;Kajikawa et al. 2008). In addition to the use of somatic embryos as a provisional screening system, we hypothesized that such embryos could be substituted for seeds for validation of gene function in the synthesis of seed components. Mature somatic embryos of soybean have been found to contain seed storage proteins (Komatsuda et al. 1992;Stejskal and Griga 1995), and the fatty acid composition of such embryos is similar to that of seeds (Shoemaker and Hammond 1988;Dahmer et al. 1991). However, relatively little information has been available regarding common features of somatic embryos and seeds.We have now investigated in detail the...

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Comparative protein accumulation patterns in soybean somatic and zygotic embryos

Cited by 29 publications

References 44 publications

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Morphogenic Aspects of Somatic Embryogenesis

Maturation of somatic embryos as a model for soybean seed development

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