Comparative profiling of extractable proteins in extracellular matrices of porcine cholecyst and jejunum intended for preparation of tissue engineering scaffolds

Muhamed, Jaseer; Rajan, A. R.; Surendran, Arun; Jaleel, Abdul; Anilkumar, T. V.

doi:10.1002/jbm.b.33567

Cited by 11 publications

(9 citation statements)

References 35 publications

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“…The CDS is known to induce an M2 macrophage predominated tissue remodeling reaction in rat subcutaneous tissue (Muhamed, Revi, Rajan, Geetha et al 2015), probably due to the differential protein content in the ECMs of gall bladder and jejunum (Muhamed et al 2017). A prominent M2 macrophage response over M1 macrophages in tissue reactions is considered as a pro-regenerative response rather than a pro-inflammatory response (Brown et al 2012).…”

Section: Discussionmentioning

confidence: 99%

An Immunopathological Evaluation of the Porcine Cholecyst Matrix as a Muscle Repair Graft in a Male Rat Abdominal Wall Defect Model

et al. 2018

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With the increasing use of animal-based biomaterials for regenerative medical applications, the need for their safety assessment is paramount. A porcine cholecyst-derived scaffold (CDS), intended as a muscle repair graft, prepared by a nondetergent/enzymatic method was engrafted in a rat abdominal wall defect model. Host tissue-scaffold interface samples were collected 2, 8, and 16 weeks postimplantation and evaluated by histopathology, immunohistochemistry, and electron microscopy. The nature of the tissue reaction was compared with those induced by a jejunum-derived scaffold (JDS) prepared by the same method and a commercial-grade small intestinal submucosa (CSIS) scaffold. A study of the immunopathological response in major lymphoid tissues and immunophenotyping for M1 and M2 macrophages was performed at the host tissue-scaffold interface. Further, "irritancy scores" for CDS and JDS were determined using CSIS as the reference material. Both CDS and JDS appeared to be potential biomaterials for muscle grafts, but the former stimulated a skeletal muscle tissue remodeling response predominated by M2 macrophages. The data support the notion that biomaterials with similar biocompatibility, based on local tissue response on implantation, may cause differential immunogenicity. Additionally, CDS compared to JDS and CSIS was found to be less immunotoxic.

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Section: Discussionmentioning

confidence: 99%

An Immunopathological Evaluation of the Porcine Cholecyst Matrix as a Muscle Repair Graft in a Male Rat Abdominal Wall Defect Model

et al. 2018

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“…Basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and transforming growth factor beta (TGFβ) were quantified after extracting the ECM proteins as per the protocol of Muhamed, Rajan, Surendran, Jaleel, and Anilkumar (). Briefly, scaffolds were powdered using Freezer mill (SPEX™ SamplePrep, 6770) dual chamber cryogenic chamber.…”

Section: Methodsmentioning

confidence: 99%

Controlled cross‐linking of porcine cholecyst extracellular matrix for preparing tissue engineering scaffold

Mony

Anilkumar

2019

J Biomed Mater Res

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Treatment with cross‐linking agents for stabilizing biomolecules is an integral step during the preparation of many extracellular matrix‐based tissue engineering scaffolds from mammalian organs. However, excess cross‐linking may cause nonavailability of biomolecules and consequent deterioration of bioinductive properties of the scaffold. The present study considered controlling the extent of cross‐linking in a porcine cholecyst extracellular matrix scaffold prepared by a nonenzymatic and nondetergent method, by ex situ incubation of the source organ in varying concentrations of neutral buffered formaldehyde (10, 4, 1 or 0%; v/v) for in situ cross‐linking of biomolecules. Reduction of the formaldehyde concentration resulted in an increase in the extent of biodegradation and a decrease in the compactness of the mesh‐like surface microarchitecture of the scaffold. Retention of collagen was maximum when treated with 10% neutral buffered formaldehyde without any variation in the content of elastin and sulphated glycosaminoglycans. Although there was a reduction in the quantity of growth factors following the cross‐linking, fibroblasts remained viable on the scaffolds. The retention of major biomolecule was maximum and autodigestion was minimum in the scaffold prepared by the ex situ treatment of cholecyst in 10% neutral buffered formalin and found suitable for preparing the tissue engineering scaffold.

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“…Lumican alters cancer progression by suppressing proliferation, migration, and invasion as well as upregulating epithelial markers and downregulating mesenchymal markers in an ER-independent fashion (Karamanou et al, 2017 ). Lumican is not likely to be incorporated into a synthetic scaffold but can be found on decellularized scaffolds (Jadalannagari et al, 2017 ; Muhamed et al, 2017 ). Lysyl oxidases (LOX) are enzymes which catalyze the cross-linking of fibrillar collagens and elastin.…”

Section: Revisiting the Underdeveloped Cues Of Breast Cancer Microenvmentioning

confidence: 99%

Engineering Breast Cancer Microenvironments and 3D Bioprinting

Belgodere

King

Bursavich

et al. 2018

Front. Bioeng. Biotechnol.

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The extracellular matrix (ECM) is a critical cue to direct tumorigenesis and metastasis. Although two-dimensional (2D) culture models have been widely employed to understand breast cancer microenvironments over the past several decades, the 2D models still exhibit limited success. Overwhelming evidence supports that three dimensional (3D), physiologically relevant culture models are required to better understand cancer progression and develop more effective treatment. Such platforms should include cancer-specific architectures, relevant physicochemical signals, stromal–cancer cell interactions, immune components, vascular components, and cell-ECM interactions found in patient tumors. This review briefly summarizes how cancer microenvironments (stromal component, cell-ECM interactions, and molecular modulators) are defined and what emerging technologies (perfusable scaffold, tumor stiffness, supporting cells within tumors and complex patterning) can be utilized to better mimic native-like breast cancer microenvironments. Furthermore, this review emphasizes biophysical properties that differ between primary tumor ECM and tissue sites of metastatic lesions with a focus on matrix modulation of cancer stem cells, providing a rationale for investigation of underexplored ECM proteins that could alter patient prognosis. To engineer breast cancer microenvironments, we categorized technologies into two groups: (1) biochemical factors modulating breast cancer cell-ECM interactions and (2) 3D bioprinting methods and its applications to model breast cancer microenvironments. Biochemical factors include matrix-associated proteins, soluble factors, ECMs, and synthetic biomaterials. For the application of 3D bioprinting, we discuss the transition of 2D patterning to 3D scaffolding with various bioprinting technologies to implement biophysical cues to model breast cancer microenvironments.

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Comparative profiling of extractable proteins in extracellular matrices of porcine cholecyst and jejunum intended for preparation of tissue engineering scaffolds

Cited by 11 publications

References 35 publications

An Immunopathological Evaluation of the Porcine Cholecyst Matrix as a Muscle Repair Graft in a Male Rat Abdominal Wall Defect Model

An Immunopathological Evaluation of the Porcine Cholecyst Matrix as a Muscle Repair Graft in a Male Rat Abdominal Wall Defect Model

Controlled cross‐linking of porcine cholecyst extracellular matrix for preparing tissue engineering scaffold

Engineering Breast Cancer Microenvironments and 3D Bioprinting

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