2013
DOI: 10.1371/journal.pgen.1003548
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Comparative Polygenic Analysis of Maximal Ethanol Accumulation Capacity and Tolerance to High Ethanol Levels of Cell Proliferation in Yeast

Abstract: The yeast Saccharomyces cerevisiae is able to accumulate ≥17% ethanol (v/v) by fermentation in the absence of cell proliferation. The genetic basis of this unique capacity is unknown. Up to now, all research has focused on tolerance of yeast cell proliferation to high ethanol levels. Comparison of maximal ethanol accumulation capacity and ethanol tolerance of cell proliferation in 68 yeast strains showed a poor correlation, but higher ethanol tolerance of cell proliferation clearly increased the likelihood of … Show more

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Cited by 75 publications
(81 citation statements)
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References 49 publications
(71 reference statements)
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“…It is likely that low to moderate ethanol concentrations have little or no effect on nutrient uptake across the plasma membrane and that, therefore, auxotrophic requirements, which strongly depend on nutrient uptake, have always remained unnoticed as an important factor in ethanol tolerance. Another QTL mapping experiment in a cross between the sake strain CBS 1585 and the laboratory BY strain identified URA3 as a causative gene for maximal ethanol accumulation capacity, but it had only a very weak contribution to ethanol tolerance for cell proliferation in the presence of 18 to 20% ethanol (45). This result may at first seem surprising, but it is not contradictory to our findings because a different genetic background was used in this study.…”
Section: Discussioncontrasting
confidence: 61%
See 1 more Smart Citation
“…It is likely that low to moderate ethanol concentrations have little or no effect on nutrient uptake across the plasma membrane and that, therefore, auxotrophic requirements, which strongly depend on nutrient uptake, have always remained unnoticed as an important factor in ethanol tolerance. Another QTL mapping experiment in a cross between the sake strain CBS 1585 and the laboratory BY strain identified URA3 as a causative gene for maximal ethanol accumulation capacity, but it had only a very weak contribution to ethanol tolerance for cell proliferation in the presence of 18 to 20% ethanol (45). This result may at first seem surprising, but it is not contradictory to our findings because a different genetic background was used in this study.…”
Section: Discussioncontrasting
confidence: 61%
“…The most widely used molecular markers in yeast are natural variations detected as hybridization differences on high-density oligonucleotide arrays (38) or determined by whole-genome sequencing (39,40). Several polygenic traits have been investigated by using these approaches, which led to the identification of loci, genes, and single nucleotide polymorphisms involved in hightemperature growth (39,40), sporulation efficiency (41), mRNA expression profiles (42), acetic acid production (43), resistance to chemical agents (44), high ethanol tolerance (34), maximal ethanol accumulation (45), low glycerol production (46,47), and thermotolerance (48).…”
Section: Discussionmentioning
confidence: 99%
“…S4A–D). Ethanol titers of 115–120 g/L have been reported previously from S288C (11), the inbred laboratory strain used here that is known for its low ethanol resistance (5, 12, 13). However, these studies were typically conducted using chemically undefined (“rich”) media.…”
mentioning
confidence: 72%
“…Previously, 15 g/L of 1-butanol was produced within engineered E.coli by deletion of fermentative pathways, thereby requiring the use of the 1-butanol pathway as the sole NADH sink under anaerobic conditions (Shen et al, 2011). While this strategy yielded 1-butanol that is regarded as one of the highest biofuel production to date, Friedlander et al, 2016;Inokuma et al, 2010;Pais et al, 2013;Wernick et al, 2016;York and Ingram, 1996) improved titers are desired. In addition to the deletion of NADH consuming pathways, many other metabolic engineering strategies have been applied in order to achieve such high titers.…”
Section: Introductionmentioning
confidence: 99%