Comparative Phytochemical and Anti-microbial Studies of Leaf, Stem, Root of Spathodea companulata

Abstract

Aim: To compare the phytochemicals and anti bacterial activities of leaf, stem and root extracts of Spathodea companulata.
 Methodology: The leaf, stem and root of Spathodea companulata were collected, washed, air-dried, ground and each extracted with water, methanol, ethyl acetate and n-hexane. The extracts were analysed for the presence of phytochemicals. Antimicrobial analysis was also carried out on the extracts.
 Results: Glycosides were present in all the extracts except stem aqueous extract. Steroids were found absent in most of the extracts except methanol stem and n-hexane root extracts. Saponins were found in methanol, aqueous and ethyl acetate extracts of the root and stem, and in methanol and aqueous leaf extracts. Alkaloids were present in methanol and aqueous extracts of the leaf and root, and in ethyl acetate and n- hexane extracts of the stem. Quantity of Alkaloids and tannins were higher in leaf, while flavonoids and glycosides were higher in the stem and the roots contained higher amount of saponins. The extraction solvent polarities played important role in the type of metabolites extracted. The stem extracts were found to be most active against the seven test organisms used.
 Conclusion: More work should be done on the isolation and identification of compounds responsible for some of the pharmacological effects of the plant parts and the subsequent development of the compounds in formulation of drugs.

Comparative Phytochemical, Proximate and In vitro Antihyperglycemic Analyses of the Stem Bark Extracts of Kigelia africana (Lam.) Benth and Annona senegalensis Pers

“…The extracts were subjected to quantitative and qualitative phytochemical analysis to determine the presence or absence of flavonoids, alkaloids, terpenoids, saponins, carbohydrates, resins, tannins, reducing sugars, glycosides, and proteins using standard phytochemical procedures described by Anarado et al [43][44][45].…”

Lipid extraction and analysis of microalgae strain Pectinodesmus PHM3 for biodiesel production

“…To test the presence of glycosides, 3ml chloroform was mixed with 10% ammonium solution and 2ml algal extract. Positive result is indicated with the development of pink color in the algal extract [47].…”

Phytochemical, Proximate, in-vitro Anti-malarial, Anti-inflammatory and Antimicrobial Screening of Leaf Extracts of Combretum platypterum (Welw) Hutch & Dalziel

“…After filtration, the residue on the filter paper is the alkaloid, which is dried in the oven at 80°C. The alkaloids content was calculated and expressed as a percentage of the weight of the sample analyzed [20,22]. % weight of Alkaloid = (W 2 -W 1 ) / weight of the sample X 100/1 (7)…”

“…The filtrate was transferred into a crucible and evaporated into dryness over a water bath and weighed to a constant weight [22].…”

“…After evaporation, the sample was dried in oven to a constant weight. The saponins content was calculated in percentage [22].…”