Comparative physiological and transcriptomic analysis reveals salinity tolerance mechanisms in Sorghum bicolor (L.) Moench

Ukwatta, Jayan; Pabuayon, Isaiah Catalino M.; Park, Jungjae; Chen, Junping; Chai, Xiaoqiang; Zhang, Heng; Zhu, Jian Kang; Xin, Zhanguo; Shi, Huazhong

doi:10.1007/s00425-021-03750-w

Cited by 9 publications

(6 citation statements)

References 91 publications

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“…Therefore, the germination and seedling stage is crucial for the establishment of plants ( Song et al, 2008 ; Vennapusa et al, 2021 ). Salt stress has serious influence on root length, especially for hypersensitive improved cultivars ( Ukwatta et al, 2021 ). It is similar to the results of our current study ( Figure 1B ).…”

Section: Discussionmentioning

confidence: 99%

Multiomics Analyses of Two Sorghum Cultivars Reveal the Molecular Mechanism of Salt Tolerance

et al. 2022

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Sorghum [Sorghum bicolor (L.) Moench] is one of the most important cereal crops and contains many health-promoting substances. Sorghum has high tolerance to abiotic stress and contains a variety of flavonoids compounds. Flavonoids are produced by the phenylpropanoid pathway and performed a wide range of functions in plants resistance to biotic and abiotic stress. A multiomics analysis of two sorghum cultivars (HN and GZ) under different salt treatments time (0, 24, 48, and 72) was performed. A total of 45 genes, 58 secondary metabolites, and 246 proteins were recognized with significant differential abundances in different comparison models. The common differentially expressed genes (DEGs) were allocated to the “flavonoid biosynthesis” and “phenylpropanoid biosynthesis” pathways. The most enriched pathways of the common differentially accumulating metabolites (DAMs) were “flavonoid biosynthesis,” followed by “phenylpropanoid biosynthesis” and “arginine and proline metabolism.” The common differentially expressed proteins (DEPs) were mainly distributed in “phenylpropanoid biosynthesis,” “biosynthesis of cofactors,” and “RNA transport.” Furthermore, considerable differences were observed in the accumulation of low molecular weight nonenzymatic antioxidants and the activity of antioxidant enzymes. Collectively, the results of our study support the idea that flavonoid biological pathways may play an important physiological role in the ability of sorghum to withstand salt stress.

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Section: Discussionmentioning

confidence: 99%

Multiomics Analyses of Two Sorghum Cultivars Reveal the Molecular Mechanism of Salt Tolerance

et al. 2022

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“…Transcriptome analysis can provide insights into the plant's response to salt stress by examining overall patterns of gene expression [32]. However, many studies on the transcriptome of salt-tolerant maize have only collected expression levels at the beginning and end of salt stress [33][34][35][36]. Therefore, we chose to investigate the dynamic global transcriptome on the root systems of seedlings at five different time points after salt treatment and compared transcriptome data from different genotypes to examine the similarities and differences in salt tolerance mechanisms of different germplasms.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Underlying Response Mechanism to Salt Stress in Maize Seedling Roots

Zhang,

Chen,

Zhang

et al. 2023

Metabolites

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Crop growth and development can be impeded by salt stress, leading to a significant decline in crop yield and quality. This investigation performed a comparative analysis of the physiological responses of two maize inbred lines, namely L318 (CML115) and L323 (GEMS58), under salt-stress conditions. The results elucidated that CML115 exhibited higher salt tolerance compared with GEMS58. Transcriptome analysis of the root system revealed that DEGs shared by the two inbred lines were significantly enriched in the MAPK signaling pathway–plant and plant hormone signal transduction, which wield an instrumental role in orchestrating the maize response to salt-induced stress. Furthermore, the DEGs’ exclusivity to salt-tolerant genotypes was associated with sugar metabolism pathways, and these unique DEGs may account for the disparities in salt tolerance between the two genotypes. Meanwhile, we investigated the dynamic global transcriptome in the root systems of seedlings at five time points after salt treatment and compared transcriptome data from different genotypes to examine the similarities and differences in salt tolerance mechanisms of different germplasms.

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“…Transcriptome analysis can scan the expression pattern of global genes and draw an overview picture to reveal the response process, which can be used to identify the mechanisms and key genes involved in salt tolerance. However, most of the transcriptome studies for salt stress in sorghum only captured the expression level at 1-3 time points [20,22,54]. Considering that the response to salt stress is a continuously dynamic process, our study provided a dynamic global transcript overview based on transcriptome sequencing at 6 time points (Table S1).…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance

Chen

Shang

Sun

et al. 2022

IJMS

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Sweet sorghum is a C4 crop that can be grown for silage forage, fiber, syrup and fuel production. It is generally considered a salt-tolerant plant. However, the salt tolerance ability varies among genotypes, and the mechanism is not well known. To further uncover the salt tolerance mechanism, we performed comparative transcriptome analysis with RNA samples in two sweet sorghum genotypes showing different salt tolerance abilities (salt-tolerant line RIO and salt-sensitive line SN005) upon salt treatment. These response processes mainly focused on secondary metabolism, hormone signaling and stress response. The expression pattern cluster analysis showed that RIO-specific response genes were significantly enriched in the categories related to secondary metabolic pathways. GO enrichment analysis indicated that RIO responded earlier than SN005 in the 2 h after treatment. In addition, we identified more transcription factors (TFs) in RIO than SN005 that were specifically expressed differently in the first 2 h of salt treatment, and the pattern of TF change was obviously different. These results indicate that an early response in secondary metabolism might be essential for salt tolerance in sweet sorghum. In conclusion, we found that an early response, especially in secondary metabolism and hormone signaling, might be essential for salt tolerance in sweet sorghum.

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Comparative physiological and transcriptomic analysis reveals salinity tolerance mechanisms in Sorghum bicolor (L.) Moench

Cited by 9 publications

References 91 publications

Multiomics Analyses of Two Sorghum Cultivars Reveal the Molecular Mechanism of Salt Tolerance

Multiomics Analyses of Two Sorghum Cultivars Reveal the Molecular Mechanism of Salt Tolerance

Comparative Transcriptome Analysis Reveals the Underlying Response Mechanism to Salt Stress in Maize Seedling Roots

Comparative Transcriptome Analysis of Two Sweet Sorghum Genotypes with Different Salt Tolerance Abilities to Reveal the Mechanism of Salt Tolerance

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