Comparative physiological and transcriptomic analyses reveal salt tolerance mechanisms of Zygosaccharomyces rouxii

Wang, Dingkang; Hao, Zhiqiang; Zhao, Jinsong; Yao, Jin; Huang, Jun; Zhou, Rongqing; Wu, Chongde

doi:10.1016/j.procbio.2019.04.009

Cited by 33 publications

(24 citation statements)

References 37 publications

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“…Glycerol production and retention under hyperosmotic stress are mediated by the Hog1 MAPK signaling cascade, which enhances glycerol synthesis. Recent reports have further confirmed that the Hog1 MAPK pathway is involved in both salt stress and sugar stress (Jiménez-Martí et al, 2011;Guo et al, 2016;Solieri et al, 2016;Wang et al, 2019). Combining recent reports with our results, we found the overlapping subset of salt-and sugar-responsive genes.…”

Section: Discussionsupporting

confidence: 90%

Genomic Insights Into Sugar Adaptation in an Extremophile Yeast Zygosaccharomyces rouxii

et al. 2020

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The osmotolerant Zygosaccharomyces rouxii is known for its trait to survive in extreme high sugar environments. This ability determines its role in the fermentation process and leads to yeast spoilage in the food industry. However, our knowledge of the gene expression in response to high sugar stress remains limited. Here, we conducted RNA-sequencing (RNA-seq) under different sugar concentrations of the spoilage yeast, Z. rouxii, which exhibit extremely high tolerance to sugar stress. The obtained differentially expressed genes (DEGs) are significantly different to that of the Saccharomyces cerevisiae, which is sensitive to extreme high sugar stress. Most of the DEGs participated in the "glucan synthesis," "transmembrane transport," "ribosome," etc. In this work, we also demonstrated that the gene ZYRO0B03476g (ZrKAR2) encoding Kar2p can significantly affect the growth of Z. rouxii under high sugar stress. In addition, we combined with a previous study on the genome sequence of Z. rouxii, indicating that several gene families contain significantly more gene copies in the Z. rouxii lineage, which involved in tolerance to sugar stress. Our results provide a gene insight for understanding the high sugar tolerance trait, which may impact food and biotechnological industries and improve the osmotolerance in other organisms.

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Section: Discussionsupporting

confidence: 90%

Genomic Insights Into Sugar Adaptation in an Extremophile Yeast Zygosaccharomyces rouxii

et al. 2020

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“…The genes for ribosome structure and biogenesis were usually associated with environmental stresses, such as acid, oxidative, heat, and salt stresses. [40][41][42][43] Thus, it may be beneficial for Blautia to survive in the harsh conditions in the host gastrointestinal tract due to the large number of stress-related genes. 44 Compared with most lactic acid bacteria, the proportion of genes related to [G] Carbohydrate transport and metabolism was relatively small in Blautia (6.57%), indicating that Blautia may have weak abilities to metabolize carbohydrates.…”

Section: Genomic and Comparative Genomic Analysis Of The Members Of Blautiamentioning

confidence: 99%

Blautia—a new functional genus with potential probiotic properties?

et al. 2021

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Blautia is a genus of anaerobic bacteria with probiotic characteristics that occur widely in the feces and intestines of mammals. Based on phenotypic and phylogenetic analyses, some species in the genera Clostridium and Ruminococcus have been reclassified as Blautia, so to date, there are 20 new species with valid published names in this genus. An extensive body of research has recently focused on the probiotic effects of this genus, such as biological transformation and its ability to regulate host health and alleviate metabolic syndrome. This article reviews the origin and biological characteristics of Blautia and the factors that affect its abundance and discusses its role in host health, thus laying a theoretical foundation for the development of new functional microorganisms with probiotic properties.

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“…1i and 1l), suggesting that the morphological changes in GXDK6 may respond to NaCl stress. Wang et al (16) reported that lipid metabolism and membrane permeability are important in regulating the morphological changes in cells. This regulation is due to the signal instructions of gene transcription and expression, which allow cells to avoid internal damage effectively in high-permeability environments.…”

Section: Resultsmentioning

confidence: 99%

Survival mechanism of a novel marine multistress-tolerant Meyerozyma guilliermondii GXDK6 under high NaCl stress as revealed by integrative omics analysis

Jiang

Cai

Sun

et al. 2021

Preprint

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A novel strain named Meyerozyma guilliermondii GXDK6 was provided in this work, which was confirmed to survive independently under high salt stress (12% NaCl) or co-stress condition of strong acid (pH 3.0) and high salts (10% NaCl) without sterilization. Its survival mechanism under high salt stress was revealed by integrated omics for the first time. Whole-genome analysis showed that 14 genes (e.g., GPD1 and FPS1) of GXDK6 relevant to salt tolerance were annotated and known to belong to various salt-resistant mechanisms (e.g., regulation of cell signal transduction and glycerol metabolism controls). Transcriptome sequencing results indicated that 1220 genes (accounting for 10.15%) of GXDK6 were differentially transcribed (p < 0.05) when GXDK6 growth was under 10% stress for 16 h, including important novel salt-tolerant-related genes (e.g., RTM1 and YHB1). Proteomics analysis demonstrated that 1005 proteins (accounting for 27.26%) of GXDK6 were differentially expressed (p < 0.05) when GXDK6 was stressed by 10% NaCl. Some of the differentially expressed proteins were defined as the novel salt-tolerant related proteins (e.g., sugar transporter STL1 and NADPH-dependent methylglyoxal reductase). Metabolomic analysis results showed that 63 types of metabolites (e.g., D-mannose, glycerol and inositol phosphate) of GXDK6 were up- or downregulated when stressed by 10% NaCl. Among them, D-mannose is one of the important metabolites that could enhance the salt-tolerance survival of GXDK6.

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Comparative physiological and transcriptomic analyses reveal salt tolerance mechanisms of Zygosaccharomyces rouxii

Cited by 33 publications

References 37 publications

Genomic Insights Into Sugar Adaptation in an Extremophile Yeast Zygosaccharomyces rouxii

Genomic Insights Into Sugar Adaptation in an Extremophile Yeast Zygosaccharomyces rouxii

Blautia—a new functional genus with potential probiotic properties?

Survival mechanism of a novel marine multistress-tolerant Meyerozyma guilliermondii GXDK6 under high NaCl stress as revealed by integrative omics analysis

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