Comparative physiological and proteomic response to phosphate deficiency between two wheat genotypes differing in phosphorus utilization efficiency

Zheng, Lu; Wang, Ruonan; Zhou, Peijun; Pan, Yilin; Shen, Renfang; Lan, Ping

doi:10.1016/j.jprot.2023.104894

Cited by 9 publications

(5 citation statements)

References 59 publications

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“…The up-regulation of GSTs in P7 indicates an adaptive response of this inbred line to the oxidative stress induced by P deficiency (Table ). The increase of GSTs was observed in various species under low P conditions, such as maize, wheat, and barley, supporting the induction of oxidative stress and response.…”

Section: Discussionmentioning

confidence: 66%

Unraveling the Mechanisms of Efficient Phosphorus Utilization in Popcorn (Zea mays L. var. everta): Insights from Proteomic and Metabolite Analysis

Barboza Bispo,

Teixeira do Amaral,

Pinto

et al. 2024

J. Proteome Res.

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The expansion of agriculture and the need for sustainable practices drives breeders to develop plant varieties better adapted to abiotic stress such as nutrient deficiency, which negatively impacts yields. Phosphorus (P) is crucial for photosynthesis and plant growth, but its availability in the soil is often limited, hampering crop development. In this study, we examined the response of two popcorn inbred lines, L80 and P7, which have been characterized previously as P-use inefficient and P-use efficient, respectively, under low (stress) and high P (control) availability. Physiological measurements, proteomic analysis, and metabolite assays were performed to unravel the physiological and molecular responses associated with the efficient use of P in popcorn. We observed significant differences in protein abundances in response to the P supply between the two inbred lines. A total of 421 differentially expressed proteins (DEPs) were observed in L80 and 436 DEPs in P7. These proteins were involved in photosynthesis, protein biosynthesis, biosynthesis of secondary metabolites, and energy metabolism. In addition, flavonoids accumulated in higher abundance in P7. Our results help us understand the major components of P utilization in popcorn, providing new insights for popcorn molecular breeding programs.

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Section: Discussionmentioning

confidence: 66%

Unraveling the Mechanisms of Efficient Phosphorus Utilization in Popcorn (Zea mays L. var. everta): Insights from Proteomic and Metabolite Analysis

Barboza Bispo,

Teixeira do Amaral,

Pinto

et al. 2024

J. Proteome Res.

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“…In the present study, the number and magnitude of DEGs in NvsCK comparison were much higher than those in SRvsCK and SRNvsCK comparisons, indicating that the addition of straw inhibited the expression levels of some N-related genes. In addition, the down-regulation of DEG genes related to K transporters and the up-regulation of DEG genes related to N transporters and P transporters responsive to nutrient shortage confirmed the important role of N and P in promoting the synthesis of key cellular molecules such as nucleic acid, protein, and ATP and maintaining the basic life activities of cells [21,22].…”

Section: Discussionmentioning

confidence: 74%

Transcriptome Analysis of Maize Ear Leaves Treated with Long-Term Straw Return plus Nitrogen Fertilizer under the Wheat–Maize Rotation System

Li,

Liu,

Zhu

et al. 2023

Plants

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Straw return (SR) plus nitrogen (N) fertilizer has become a practical field management mode to improve soil fertility and crop yield in North China. This study aims to explore the relationship among organic waste, mineral nutrient utilization, and crop yield under SRN mode. The fertilizer treatments included unfertilized (CK), SR (straws from wheat and corn), N fertilizer (N), and SR plus N fertilizer (SRN). SRN treatment not only significantly increased the grain yield, net photosynthetic rate, and transpiration rate but also enhanced the contents of chlorophyll, soluble sugar, and soluble protein and increased the activities of antioxidant enzymes but reduced intercellular CO2 concentration and malondialdehyde (MDA) content when compared to other treatments. There were 2572, 1258, and 3395 differentially expressed genes (DEGs) identified from the paired comparisons of SRvsCK, NvsCK, and SRNvsCK, respectively. The transcript levels of many promising genes involved in the transport and assimilation of potassium, phosphate, and nitrogen, as well as the metabolisms of sugar, lipid, and protein, were down-regulated by straw returning under N treatment. SRN treatment maintained the maximum maize grain yield by regulating a series of genes’ expressions to reduce nutrient shortage stress and to enhance the photosynthesis of ear leaves at the maize grain filling stage. This study would deepen the understanding of complex molecular mechanisms among organic waste, mineral nutrient utilization, crop yield, and quality.

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“…This approach sheds light on the metabolic effects of Pi deficiency in wheat, revealing alterations in pathways related to carbohydrates, amino acids, and secondary metabolism 26 , 27 . Additionally, proteomic analysis revealed significant alterations in the abundance of proteins associated with nitrogen and phosphorus, small molecule, and carboxylic acid metabolic processes in response to Pi deficiency in wheat 28 . Plant hormones, such as auxin, cytokinin (CK), ethylene (ETH), abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), and salicylic acid (SA), undergo changes that play crucial roles in integrating Pi signaling and regulating root growth 29 – 31 .…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic and physiological analyses unravel wheat source root adaptation to phosphorous deficiency

Luo,

Usman,

Pang

et al. 2024

Sci Rep

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Phosphorus (P) is a crucial macronutrient for plant growth and development. Basic metabolic processes regulate growth; however, the molecular detail of these pathways under low phosphorous (LP) in wheat is still unclear. This study aims to elucidate the varied regulatory pathways responses to LP stress in wheat genotypes. Phenotypic, physiological, and transcriptome analyses were conducted on Fielder (P efficient) and Ardito (P inefficient) wheat genotypes after four days of normal phosphorous (NP) and LP stress. In response to LP, Fielder outperformed Ardito, displaying higher chlorophyll content-SPAD values (13%), plant height (45%), stem diameter (12%), shoot dry weight (42%), and root biomass (75%). Root structure analysis revealed that Fielder had greater total root length (50%), surface area (56%), volume (15%), and diameter (4%) than Ardito under LP. These findings highlight Fielder’s superior performance and adaptation to LP stress. Transcriptome analysis of wheat genotype roots identified 3029 differentially expressed genes (DEGs) in Fielder and 1430 in Ardito, highlighting LP-induced changes. Key DEGs include acid phosphatases (PAPs), phosphate transporters (PHT1 and PHO1), SPX, and transcription factors (MYB, bHLH, and WRKY). KEGG enrichment analysis revealed key pathways like plant hormones signal transduction, biosynthesis of secondary metabolites, and carbohydrate biosynthesis metabolism. This study unveils crucial genes and the intricate regulatory process in wheat’s response to LP stress, offering genetic insights for enhancing plant P utilization efficiency.

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Comparative physiological and proteomic response to phosphate deficiency between two wheat genotypes differing in phosphorus utilization efficiency

Cited by 9 publications

References 59 publications

Unraveling the Mechanisms of Efficient Phosphorus Utilization in Popcorn (Zea mays L. var. everta): Insights from Proteomic and Metabolite Analysis

Unraveling the Mechanisms of Efficient Phosphorus Utilization in Popcorn (Zea mays L. var. everta): Insights from Proteomic and Metabolite Analysis

Transcriptome Analysis of Maize Ear Leaves Treated with Long-Term Straw Return plus Nitrogen Fertilizer under the Wheat–Maize Rotation System

Comparative transcriptomic and physiological analyses unravel wheat source root adaptation to phosphorous deficiency

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