Comparative physical mapping of the 5S and 18S-25S rDNA in nine wild Hordeum species and cytotypes

Taketa, Shin; Harrison, G. E.; Heslop-Harrison, J. S.

doi:10.1007/s001220051033

Cited by 128 publications

(87 citation statements)

References 28 publications

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“…Since the positions of rDNA sequences provide useful markers for chromosome identification, physical mapping of rDNA has been reported in many plant species including bryophyte (Fukui et al 1994, Lubaret et al 1996, Fukui et al 1998, Roose et al 1998, Cerban et al 1999, Moscone et al 1999, Taketa et al 1999, Sone et al 1999, Siroky et al 2001, Tagashira and Kondo 2001, Shibata et al 2004. Although the locations of 5S rDNA loci differ from the locations of 45S rDNA loci in most plant species, the 5S rDNA and 45S rDNA loci in several other plant species are located in close proximity (Roose et al 1998, Taketa et al 1999.…”

Section: Fish With 5s and 26s Rdna Probesmentioning

confidence: 99%

“…Although the locations of 5S rDNA loci differ from the locations of 45S rDNA loci in most plant species, the 5S rDNA and 45S rDNA loci in several other plant species are located in close proximity (Roose et al 1998, Taketa et al 1999. Sone et al (1999) reported the insertion of the 5S rDNA sequence within the 45S rDNA repeat sequence in the chromosomes of Marchantia polymorpha, a species of bryophyte.…”

Section: Fish With 5s and 26s Rdna Probesmentioning

confidence: 99%

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Interstitial Telomere-like Repeats in the Haplopappus gracilis (Asteraceae) Genome Revealed by Fluorescence in situ Hybridization

Hanmoto¹,

Kataoka

Ohmido

et al. 2007

CYTOLOGIA

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Summary Fluorescence in situ hybridization (FISH) using an Arabidopsis-type telomeric sequence (TTTAGGG) n probe to the mitotic chromosomes of Haplopappus gracilis (nϭ2) revealed the presence of Interstitial Telomere-like Repeats (ITRs) in at the subdistal position of the long arm of both chromosome pairs (1g and 2g). The H. gracilis genome (nϭ2) is generally thought to reconstitute from the nϭ4 complement of the allied species, H. ravenii. The sites we identified by FISH are in close proximity to the chromosomal rearrangement fusion points. This evidence supports the hypothesis that the karyotype of H. garacilis evolved from that of H. ravenii due to chromosome breakage and the subsequent end-to-end chromosome fusion.

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Section: Fish With 5s and 26s Rdna Probesmentioning

confidence: 99%

Section: Fish With 5s and 26s Rdna Probesmentioning

confidence: 99%

Interstitial Telomere-like Repeats in the Haplopappus gracilis (Asteraceae) Genome Revealed by Fluorescence in situ Hybridization

Hanmoto¹,

Kataoka

Ohmido

et al. 2007

CYTOLOGIA

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“…For instance, Trifolium genomic reorganization may occur during speciation and explain species-specific distribution patterns of 5S and 18S (Ansari et al 1999). There is other evidence of the large amount of variation in the numbers and locations of ribosomal genes between closely related species in certain genera (Taketa et al 1999;Adams et al 2000), between different individuals in some species (Frello and HeslopHarrison 2000;Luo et al 2006), and even within individual plants in Allium (Schubert and Wobus 1985), suggesting that the number and location of rDNA can change readily.…”

Section: Discussionmentioning

confidence: 99%

A dynamic evolution of chromosome in subgenus Potamogeton revealed by physical mapping of rDNA loci detection

et al. 2012

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In this study, we analyzed 12 species in the subgenus Potamogeton (8 tetraploids and 2 diploids, 2 putative tetraploid hybrids) at the chromosomal level, including counting the chromosome number and physically mapping the rDNA. The extent of variation in the chromosome number and rDNA loci was determined in the Potamogeton species on both the inter-and intra-specific level. Moreover, one of the hybrid sets (P. perfoliatus # 9 P. wrightii $) was picked for performing artificial pollination for producing F1 generation adults and for sequential morphological character analysis and FISH detection. After comparing the parental species and natural hybrids from three different geographic locations, the intraspecific variations of rDNA loci were revealed in sampled plants; in addition, comparisons between artificial F1 and natural hybrids (P. 9 intortusifolius) showed a rapid change of 45S rDNA loci in response to the interspecific hybridization. We also compared our results concerning rDNA patterns with phylogenetic documents to derive complementary clues to karoytype evolution and interspecific relationships. Based on frequent hybridizations and active clonal reproduction with weak genetic selection, the rDNA chromosomal repatterning, such as the gain or loss of rDNA loci together with rDNA movements, might be one trend of chromosome evolution in this genus.

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“…rDNA sequences are also useful chromosome landmarks and provide important information about genome evolution at the chromosomal level. In some species, such as Arabidopsis thaliana [7], Hordeum vulgare [8] and Rhoeo spathacea [9,10], fluorescence in situ hybridisation (FISH) with 25S and 5S rDNA as probes is sufficient to identify each chromosome in the complement. A complementary approach that can be used for karyotype characterization is double fluorescent banding with DAPI and CMA fluorochromes, which discriminates between ATand GC-rich DNA regions.…”

Section: Introductionmentioning

confidence: 99%

Intraspecific polymorphism of ribosomal DNA loci number and morphology in Brachypodium pinnatum and Brachypodium sylvaticum

Breda

Wolny

Hasterok

2012

Cellular and Molecular Biology Letters

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Abbreviations used: Ag-NOR -silver-binding nucleolar organizer region; BAC -bacterial artificial chromosome; CMA+ -chromomycin A 3 positive bands; FISH -fluorescence in situ hybridisation; GISH -genomic in situ hybridisation; ITS -internal transcribed spacer; NOR -nucleolar organizer region; NTS -non-transcribed spacer; rDNA -ribosomal DNA Abstract: The genus Brachypodium has become the target of extensive cytomolecular studies since one of its representatives, B. distachyon, has been accepted as a model plant for temperate cereals and forage grasses. Recent preliminary studies suggested that intraspecific rDNA polymorphism can occur in at least two members of the genus, B. sylvaticum and B. pinnatum, so the aim of this study was to further analyse this phenomenon. FISH with 25S rDNA and 5S rDNA probes was performed on somatic metaphase chromosomes, supplemented by the silver staining technique which distinguishes transcriptionally active from inactive 18S-5.8S-25S rDNA loci. The number, size and chromosomal distribution of 5S rDNA loci were very constant: two loci were invariably observed in all studied diploid accessions of both species, while four 5S rDNA loci were present in the tetraploid B. pinnatum. In contrast to 5S rDNA loci, those of the 35S rDNA were more variable. Two or three loci were observed in the diploid B. pinnatum and four in tetraploid accessions. In chromosome complements of B. sylvaticum accessions from two to six 35S rDNA sites were detected. Regardless of total rDNA locus number, only two were transcriptionally active in diploid accessions of both species, while two or four were active in the tetraploid B. pinnatum. Additionally, the fluorescent CMA/DAPI banding method was used to identify the relation between rDNA sites and CMA+ bands. It was revealed that the number and chromosomal distribution of CMA+ bands are in congruence only with 35S rDNA loci which gave strong FISH signals.

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Comparative physical mapping of the 5S and 18S-25S rDNA in nine wild Hordeum species and cytotypes

Cited by 128 publications

References 28 publications

Interstitial Telomere-like Repeats in the Haplopappus gracilis (Asteraceae) Genome Revealed by Fluorescence in situ Hybridization

Interstitial Telomere-like Repeats in the Haplopappus gracilis (Asteraceae) Genome Revealed by Fluorescence in situ Hybridization

A dynamic evolution of chromosome in subgenus Potamogeton revealed by physical mapping of rDNA loci detection

Intraspecific polymorphism of ribosomal DNA loci number and morphology in Brachypodium pinnatum and Brachypodium sylvaticum

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