Comparative phylogenetic analysis of aquaporins provides insight into the gene family expansion and evolution in plants and their role in drought tolerant and susceptible chickpea cultivars

Background Legumes comprise important crops that offer major agronomic benefits, including the capacity of establishing symbiosis with rhizobia, fixing atmospheric N2. It has been proven that legumes are particularly susceptible to boron (B) stress, which leads to important yield penalties. Boron (B) deficiency or toxicity in plants causes the inhibition of growth and an altered development. Under such conditions, the participation of two distinct protein families (the major intrinsic protein family MIP and the Boron transporter family BOR) is required to minimize detrimental effects caused by B stress. However, in legumes, little is known about the transport mechanisms responsible for B uptake and distribution, especially under deficiency. Results A Medicago truncatula protein, MtNIP5;1 (Medtr1g097840) (homologous to the Arabidopsis thaliana AtNIP5;1) was identified as a novel legume B transporter involved in B uptake under deficiency. Further analyses revealed that this M. truncatula aquaporin expression was boron-regulated in roots, being induced under deficiency and repressed under toxicity. It localizes at the plasma membrane of root epidermal cells and in nodules, where B plays pivotal roles in symbiosis. Furthermore, the partial complementation of the nip5;1–1 A. thaliana mutant phenotype under B deficiency supports a functional role of MtNIP5;1 as a B transporter in this legume model plant. Conclusions The results here presented support a functional role of MtNIP5;1 in B uptake under deficiency and provides new insights into B transport mechanisms in legume species.

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“…2c). This supports a shared role between these two proteins transporting boric acid across the plasma membrane [36,39,40]..…”

Section: Identification Of Atnip5;1 Homologs In M Truncatulasupporting

confidence: 57%

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

2020

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show abstract

“…2C) supporting a shared role between these two proteins transporting boric acid across the plasma membrane. (36,39,40).…”

Section: Identi Cation Of Atnip5;1 Homologs In M Truncatulamentioning

confidence: 99%

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

Granado‐Rodríguez

Bolaños

Reguera

2020

Preprint

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Background: Boron (B) deficiency or toxicity in soils can cause the inhibition of growth and an altered development in plants. In such conditions, the participation of two distinct protein families (the aquaporin family Major intrinsic proteins (MIPs) and the Boron transporter family BOR) is required to minimize detrimental effects caused by B stress. Legumes comprise important crops that offer major agronomic benefits, including the capacity of establishing symbiosis with rhizobia fixing atmospheric N2 and for being important food protein sources. It has been proven that legumes are susceptible to B stress, which leads to important yield penalties. However, little is known about the transport mechanisms responsible for B uptake and distribution in legumes, especially under deficiency.Results: A Medicago truncatula protein, MtNIP5;1 (Medtr1g097840) (homologous to the Arabidopsis thaliana AtNIP5;1) was identified as a novel legume B transporter involved in B uptake under deficiency. Further analyses revealed that this M. truncatula aquaporin expression was boron-regulated in roots, being induced under deficiency and repressed under toxicity. It localizes at the plasma membrane of root epidermal cells and in nodules, where B plays pivotal roles in symbiosis. Furthermore, a partial complementation of the nip5;1 A. thaliana mutant phenotype under B deficiency supports a functional role of MtNIP5;1 as a B transporter in this legume model plant. Conclusions: The results here presented support a functional role of MtNIP5;1 in B uptake under deficiency and provides new insights into this micronutrient transport mechanisms in legume species.

show abstract

“…This supports a shared role between these two proteins transporting boric acid across the plasma membrane. (36,39,40).…”

Section: Identi Cation Of Atnip5;1 Homologs In M Truncatulamentioning

confidence: 99%

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

Granado‐Rodríguez

Bolaños

Reguera

2020

Preprint

View full text Add to dashboard Cite

Background: Legumes comprise important crops that offer major agronomic benefits, including the capacity of establishing symbiosis with rhizobia, fixing atmospheric N2. It has been proven that legumes are particularly susceptible to boron (B) stress, which leads to important yield penalties. Boron (B) deficiency or toxicity in plants causes the inhibition of growth and an altered development. Under such conditions, the participation of two distinct protein families (the major intrinsic protein family MIP and the Boron transporter family BOR) is required to minimize detrimental effects caused by B stress. However, in legumes, little is known about the transport mechanisms responsible for B uptake and distribution, especially under deficiency. Results: A Medicago truncatula protein, MtNIP5;1 (Medtr1g097840) (homologous to the Arabidopsis thaliana AtNIP5;1) was identified as a novel legume B transporter involved in B uptake under deficiency. Further analyses revealed that this M. truncatula aquaporin expression was boron-regulated in roots, being induced under deficiency and repressed under toxicity. It localizes at the plasma membrane of root epidermal cells and in nodules, where B plays pivotal roles in symbiosis. Furthermore, the partial complementation of the nip5;1-1 A. thaliana mutant phenotype under B deficiency supports a functional role of MtNIP5;1 as a B transporter in this legume model plant. Conclusions: The results here presented support a functional role of MtNIP5;1 in B uptake under deficiency and provides new insights into B transport mechanisms in legume species.

show abstract

Comparative phylogenetic analysis of aquaporins provides insight into the gene family expansion and evolution in plants and their role in drought tolerant and susceptible chickpea cultivars

Cited by 29 publications

References 113 publications

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

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