Comparative phylo-pangenomics reveals generalist lifestyles in representative Acinetobacter species and proposes candidate gene markers for species identification

Almeida, Otávio Guilherme Gonçalves de; Furlan, João Pedro Rueda; Stehling, Eliana Guedes; Martinis, Elaine Cristina Pereira De

doi:10.1016/j.gene.2021.145707

Cited by 8 publications

(8 citation statements)

References 78 publications

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“…This finding was speculated to be caused by competition between LAB, and the growth of dominant LAB might inhibit the metabolic activities of LAB with low abundance. The addition of glucose reduced the relative abundance of some pathogenic bacteria, including Escherichia–Shigella and Acinetobacter ( Liu et al, 2020 ; De Almeida et al, 2021 ). Similarly, Acinetobacter was downregulated by B. subtilis .…”

Section: Discussionmentioning

confidence: 99%

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

et al. 2022

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This study was conducted to investigate the effects of different additives on the fermentation quality, nutrient composition, bacterial communities, and metabolic profiles of the silage of hybrid Pennisetum. The experiment was conducted using five treatments, i.e., CK, control group, MA, 1% malic acid of fresh matter (FM) basis, GL, 1% glucose of FM basis, CE, 100 U/g FM cellulase, and BS, 106 cfu/g FM Bacillus subtilis, with six replicates each treatment. After a 120-day fermentation, 30 silage packages were opened for subsequent determination. As a result, all four additives had positive effects on the fermentation quality and nutrient composition of the silage of hybrid Pennisetum. The high-throughput sequencing of V3–V4 regions in 16S rRNA was performed, and results showed that Firmicutes and Proteobacteria were the dominant phyla and that Aquabacterium and Bacillus were the dominant genera. MA, GL, CE, and BS treatment resulted in 129, 21, 25, and 40 differential bacteria, respectively. The four additives upregulated Bacillus smithii but downregulated Lactobacillus rossiae. Metabolic profiles were determined by UHPLC-Q/TOF-MS technology and the differential metabolites caused by the four additives were 47, 13, 47, and 18, respectively. These metabolites played antioxidant, antibacterial, and anti-inflammatory functions and involved in pathways, such as the citrate cycle, carbon fixation in photosynthetic organisms, and glyoxylate and dicarboxylate metabolism. In conclusion, silage additives promoted fermentation quality and nutrient composition by altering bacterial communities and metabolic profiles. This study provided potential biomarkers for the improvement of silage quality.

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Section: Discussionmentioning

confidence: 99%

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

et al. 2022

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“…Previous studies disclosed that Acinetobacter resistance to toxins derives from plasmids that carry functional genes within strains [ 60 , 61 ]. Noticeably, the AS23 strain genome identified here did not exhibit an extrachromosomal plasmid (Figure S 3 ), indicating its resistance to TS regulated entirely through its chromosome.…”

Section: Discussionmentioning

confidence: 99%

“…Noticeably, the AS23 strain genome identified here did not exhibit an extrachromosomal plasmid (Figure S 3 ), indicating its resistance to TS regulated entirely through its chromosome. Acinetobacter is known to degrade toxins or tolerate toxic compounds within host guts and soil [ 60 ]. This study also showed that AS23 in liquid medium can use TS as a single carbon source during fermentation, effectively degrading TS (Fig.…”

Section: Discussionmentioning

confidence: 99%

Soil-derived bacteria endow Camellia weevil with more ability to resist plant chemical defense

Zhang

Shu

et al. 2022

Microbiome

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Background Herbivorous insects acquire their gut microbiota from diverse sources, and these microorganisms play significant roles in insect hosts’ tolerance to plant secondary defensive compounds. Camellia weevil (Curculio chinensis) (CW) is an obligate seed parasite of Camellia oleifera plants. Our previous study linked the CW’s gut microbiome to the tolerance of the tea saponin (TS) in C. oleifera seeds. However, the source of these gut microbiomes, the key bacteria involved in TS tolerance, and the degradation functions of these bacteria remain unresolved. Results Our study indicated that CW gut microbiome was more affected by the microbiome from soil than that from fruits. The soil-derived Acinetobacter served as the core bacterial genus, and Acinetobacter sp. was putatively regarded responsible for the saponin-degradation in CW guts. Subsequent experiments using fluorescently labeled cultures verified that the isolate Acinetobacter sp. AS23 can migrate into CW larval guts, and ultimately endow its host with the ability to degrade saponin, thereby allowing CW to subsist as a pest within plant fruits resisting to higher concentration of defensive chemical. Conclusions The systematic studies of the sources of gut microorganisms, the screening of taxa involved in plant secondary metabolite degradation, and the investigation of bacteria responsible for CW toxicity mitigation provide clarified evidence that the intestinal microorganisms can mediate the tolerance of herbivorous insects against plant toxins.

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“…The genomes of Acinetobacter pittii , A. lactucae , A. oleivorans , A. calcoaceticus , A. nosocomiali , A. seifertii and A. baumannii were included for phylogenetic analysis indicating AS23 resided in clade II ( Fig. 2B ) ( 28 ). To confirm the genetic difference between AS23 and similar species, we described the genomic collinearity and K a / K s values ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…2E and F ). Notably, A. lactucae and A. baumannii were reported as members of the “Acb clade” ( 29 ) consisting of at least 7 species ( A. baumannii , A. calcoaceticus , A. lactucae , A. nosocomialis , A. oleivorans , A. pittii , and A. seifertii ) ( 28 ). The genome published here, along with the assessment of antibiotic resistance and TS tolerance, provides a starting point for our studies on gut bacterial degradation of TS ability.…”

Section: Discussionmentioning

confidence: 99%

Specific Enriched Acinetobacter in Camellia Weevil Gut Facilitate the Degradation of Tea Saponin: Inferred from Bacterial Genomic and Transcriptomic Analyses

Huang

He³

et al. 2022

Microbiol Spectr

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Comparative phylo-pangenomics reveals generalist lifestyles in representative Acinetobacter species and proposes candidate gene markers for species identification

Cited by 8 publications

References 78 publications

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

Soil-derived bacteria endow Camellia weevil with more ability to resist plant chemical defense

Specific Enriched Acinetobacter in Camellia Weevil Gut Facilitate the Degradation of Tea Saponin: Inferred from Bacterial Genomic and Transcriptomic Analyses

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