Comparative phosphoproteome analysis of Magnaporthe oryzae-responsive proteins in susceptible and resistant rice cultivars

Li, Yunfeng; Ye, Zhijian; Nie, Yongzhan; Zhang, Jian; Wang, Guoliang; Wang, Zhenzhong

doi:10.1016/j.jprot.2014.12.007

Cited by 34 publications

(26 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Reversible protein phosphorylation is a major mechanism of signal transduction that is mediated by protein kinases and protein phosphatases, thereby regulating many biological processes [ 18 ]. Moreover, this PTM has been reported to be associated with reactions toward abiotic and biotic factors (e.g., defense responses against pathogens) [ 19 ]. Phosphorylation changes in the host proteome under virus infection are investigated more thoroughly in humans or animals than in plants.…”

Section: Introductionmentioning

confidence: 99%

Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in N. benthamiana Infected Plants at the Early Stage of the Infection

Wrzesińska

Gevaert

et al. 2018

IJMS

View full text Add to dashboard Cite

Signaling in host plants is an integral part of a successful infection by pathogenic RNA viruses. Therefore, identifying early signaling events in host plants that play an important role in establishing the infection process will help our understanding of the disease process. In this context, phosphorylation constitutes one of the most important post-translational protein modifications, regulating many cellular signaling processes. In this study, we aimed to identify the processes affected by infection with Peanut stunt virus (PSV) and its satellite RNA (satRNA) in Nicotiana benthamiana at the early stage of pathogenesis. To achieve this, we performed proteome and phosphoproteome analyses on plants treated with PSV and its satRNA. The analysis of the number of differentially phosphorylated proteins showed strong down-regulation in phosphorylation in virus-treated plants (without satRNA). Moreover, proteome analysis revealed more down-regulated proteins in PSV and satRNA-treated plants, which indicated a complex dependence between proteins and their modifications. Apart from changes in photosynthesis and carbon metabolism, which are usually observed in virus-infected plants, alterations in proteins involved in RNA synthesis, transport, and turnover were observed. As a whole, this is the first community (phospho)proteome resource upon infection of N. benthamiana with a cucumovirus and its satRNA and this resource constitutes a valuable data set for future studies.

show abstract

Section: Introductionmentioning

confidence: 99%

Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in N. benthamiana Infected Plants at the Early Stage of the Infection

Wrzesińska

Gevaert

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…Proteomic-based approaches provide powerful tools for investigation of complex biological processes (Franco et al, 2013 ). Moreover, quantitative phospho- and acetyl proteomics analyses have been successfully applied in many studies including the mechanism of aestivation in sea cucumbers (Chen et al, 2016 ), regulation of resistant rice (Li et al, 2015 ), regulation of development in the murine brain (Goswami et al, 2012 ), regulation of mouse cardiomyopathy (Kuzmanov et al, 2016 ), and pathological processes in type 2 diabetes (Du et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers

Sun

Lin

et al. 2018

Front. Physiol.

View full text Add to dashboard Cite

Sea cucumbers exposed to stressful circumstances eviscerate most internal organs, and then regenerate them rapidly under favorable environments. Reversible protein phosphorylation and acetylation are major modifications regulating protein function. Herein, for the first time, we perform quantitative phospho- and acetyl proteomics analyses of intestine regeneration in a sea cucumber species Apostichopus japonicus. We identified 1,862 phosphorylation sites in 1,169 proteins, and 712 acetylation sites in 470 proteins. Of the 147 and 251 proteins differentially modified by phosphorylation and acetylation, respectively, most were related to cytoskeleton biogenesis, protein synthesis and modification, signal recognition and transduction, energy production and conversion, or substance transport and metabolism. Phosphorylation appears to play a more important role in signal recognition and transduction than acetylation, while acetylation is of greater importance in posttranslational modification, protein turnover, chaperones; energy production and conversion; amino acid and lipid transport and metabolism. These results expanded our understanding of the regulatory mechanisms of posttranslational modifications in intestine regeneration of sea cucumbers after evisceration.

show abstract

“…In recent years, fluorescent proteins have been used to monitor living organisms, such as Pseudomonas syringae, X. axonopodis pv. dieffenbachiae, and Xylella fastidiosa, in their hosts [24,25]. Applications of fluorescent marker systems can facilitate the detection of invading pathogens, and monitor the migration and proliferation of the bacteria.…”

Section: Introductionmentioning

confidence: 99%

Western Blot Detection of Xanthomonas Oryzae pv. Oryzae in Rice

Lan¹,

Guan²

2015

J Plant Pathol Microbiol

View full text Add to dashboard Cite

Comparative phosphoproteome analysis of Magnaporthe oryzae-responsive proteins in susceptible and resistant rice cultivars

Cited by 34 publications

References 51 publications

Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in N. benthamiana Infected Plants at the Early Stage of the Infection

Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in N. benthamiana Infected Plants at the Early Stage of the Infection

Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers

Western Blot Detection of Xanthomonas Oryzae pv. Oryzae in Rice

Contact Info

Product

Resources

About