Comparative phenotypic characterization identifies few differences in the metabolic capacity between Escherichia coli ST131 subclones

Alqasim, Abdulaziz; Jaffal, Ahmad Abu; Almutairi, Naif; Alyousef, Abdullah A.

doi:10.1016/j.sjbs.2020.11.008

Cited by 5 publications

(12 citation statements)

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“…Metabolism is an important factor used by bacteria to enhance their colonization of hosts [ 35 ], and it has been shown that humans could be exploited by many bacterial pathogens as a rich source of nutrients to aid their survival and growth [ 36 ]. Given the rapid global dissemination of particular MDR ExPEC clones, such as ST131, previous studies have explored the role of metabolism in driving the success of these clones [ 28 , 31 , 32 ]. However, these reports were limited to UPEC population and the metabolic traits of ExPEC isolates from the bacteremia population have poorly been studied.…”

Section: Discussionmentioning

confidence: 99%

“…We previously found very high utilization levels for these two substrates among E . coli urine isolates [ 31 ]. In this regard, previous comparative phenotypic microarrays analysis of 10 isolates representing the major ExPEC STs, including 190 different substrates, found that bacteremia isolates were associated with lower metabolic activity compared with those isolated from urine [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…We previously characterized a collection of E. coli blood isolates from Saudi Arabia and demonstrated that ST131 accounted for 54.8% of all isolates, 88.2% of which were ESBL-producing [ 23 ]. Many previous local and international studies have focused on determining and comparing the metabolic potential of uropathogenic E. coli (UPEC) clones [ 28 , 31 , 32 ]. For instance, our research group has recently published a comparative metabolic analysis, on a panel of E. coli isolates from urine population, showing no unique metabolic potential of ST131 [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

“…Many previous local and international studies have focused on determining and comparing the metabolic potential of uropathogenic E. coli (UPEC) clones [ 28 , 31 , 32 ]. For instance, our research group has recently published a comparative metabolic analysis, on a panel of E. coli isolates from urine population, showing no unique metabolic potential of ST131 [ 31 ]. However, little is known about metabolic traits of E. coli bacteremia population, particularly those for ST131.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Metabolic Characterization of Extraintestinal Pathogenic Escherichia coli Blood Isolates from Saudi Arabia

Alangari

Jaffal

Alyousef

et al. 2022

Journal of Tropical Medicine

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Background. The prevalence of bloodstream infections caused by extraintestinal pathogenic Escherichia coli (ExPEC) has increased substantially. E. coli ST131 is one of the dominant ExPEC clones among E. coli bacteremia population. Metabolism can trigger the pathogenesis of some bacterial isolates, and here we evaluated and compared the metabolic traits of E. coli bacteremia isolates including β-lactamase (BL)/extended-spectrum β-lactamase (ESBL)-positive and ESBL-negative isolates and ST131 and non-ST131 isolates. Methods. The metabolic profiles of thirty E. coli isolates, obtained from blood samples for hospitalized individuals at a tertiary healthcare facility in Riyadh, were determined using HiMedia carbohydrate test strips. The difference in the utilization ability between isolate groups was then statistically assessed. Results. Our data found that non-BL/ESBL producers were of low metabolic capacity compared with ESBL-positive isolates although the difference remained insignificant. Higher levels of utilization for some carbohydrates, such as fructose and trehalose, were detected among ST131 isolates when compared with non-ST131, and ST131 was also significantly associated with metabolizing rhamnose. The mean bio-score of both isolate groups was insignificant. We showed no link between metabolism and antimicrobial susceptibility profiles among tested blood isolates. Conclusion. ST131 blood isolates were slightly higher in their carbohydrate utilization activity than non-ST131. More importantly, ST131 isolates were significantly capable of metabolizing rhamnose. Future research should focus on the factors that might drive the success of major ExPEC clones such as ST131.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative Metabolic Characterization of Extraintestinal Pathogenic Escherichia coli Blood Isolates from Saudi Arabia

Alangari

Jaffal

Alyousef

et al. 2022

Journal of Tropical Medicine

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“…11 We p re v i o u s l y d e te r m i n e d t h e antimicrobial sensitivity, ESBL production, ST131 clonal status, virulence associated gene carriage and metabolic potential of a panel of clinical UPEC isolates. [14][15][16] The identification of plasmid replicon types is important in understanding the epidemiological perspective of prevalence and transmission of ESBL encoding genes in E. coli, and this can help in tracking their origin by comparing them to environmental or animal isolates and in the diagnosis of clinically important ESBL-producing E. coli. 17 Given that information on plasmid replicon diversity of clinical E. coli isolates is very scarce in Saudi Arabia, this study sought to describe and compare the replicon diversity of clinical E. coli urine isolates, including ST131 and non-ST131, ESBL-positive and ESBL-negative, and different ST131 subclones.…”

Section: Introductionmentioning

confidence: 99%

Plasmid Replicon Diversity of Clinical Uropathogenic Escherichia coli Isolates from Riyadh, Saudi Arabia

Alqasim¹,

Jaffal²,

Almutairi³

et al. 2022

J. Pure Appl. Microbiol.

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The aim of this study was to identify and compare the plasmid replicons of clinical uropathogenic Escherichia coli (UPEC) isolates, involving extended spectrum β-lactamase (ESBL)-positive and ESBL-negative, E. coli ST131 and non-ST131 and various ST131 subclones. Plasmid replicon typing on 24 clinical UPEC isolates was carried out using polymerase chain reaction-based replicon typing. A statistical analysis was performed to assess the associations between plasmid replicon types and ESBL carriage, and to evaluate the link between ST131 isolates and high replicon carriage. Eight replicons, I1α, N2, Iγ, X1, FIIS, K, FIA, and FII were detected. The FII was the most common replicon identified here. ESBL-positive E. coli isolates were highly associated with I1α, N2, Iγ, X1, and FIIS replicons, while FIA was present only in ESBL-negative group. ST131 isolates were highly associated with I1α and N2 replicons compared to non-ST131. No link was found between replicon carriage and the number or type of ESBLs in E. coli isolates. The diversity observed in replicon patterns of our clinical E. coli isolates indicates that they might be originated from different sources. The presence of replicons reported previously in animal sources suggests a possible transfer of antimicrobial resistance between animal and human bacterial isolates.

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Comparative genomics and phenotypic studies to determine site-specificity of Escherichia coli in the lower gastrointestinal tract of humans

et al. 2023

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Escherichia coli ( E. coli ) is an important commensal in the human gut; however, it is unknown whether strains show site-specificity in the lower gut. To investigate this, we assessed genotypic and phenotypic differences in 37 clone pairs (two strains with very similar multiple locus variable-number-tandem-repeat analysis [MLVA] profiles) of E. coli isolated from mucosal biopsies of two different gut locations (terminal ileum and rectum). The clone pairs varied at the genomic level; single nucleotide polymorphisms (SNPs) were common, multiple nucleotide polymorphisms (MNPs) were observed but less common, and few indels (insertions and deletions) were detected. The variation was higher in clone pairs that are associated with non-human-associated sequence types (ST) compared to human-associated STs, such as ST95, ST131, and ST73. No gene(s) with non-synonymous mutations were found to be commonly associated with either the terminal ileum or the rectal strains. At the phenotypic level, we identified the metabolic signatures for some STs. Rectum strains of some STs showed consistently higher metabolic activity with particular carbon sources. Clone pairs belonging to specific STs showed distinct growth patterns under different pH conditions. Overall, this study showed that E . coli may exhibit genomic and phenotypic variability at different locations in the gut. Although genomics did not reveal significant information suggesting the site-specificity of strains, some phenotypic studies have suggested that strains may display site-specificity in the lower gut. These results provide insights into the nature and adaptation of E. coli in the lower gut of humans. To the best of our knowledge, no study has investigated or demonstrated the site-specificity of commensal E. coli in the human gut.

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Comparative phenotypic characterization identifies few differences in the metabolic capacity between Escherichia coli ST131 subclones

Cited by 5 publications

References 20 publications

Comparative Metabolic Characterization of Extraintestinal Pathogenic Escherichia coli Blood Isolates from Saudi Arabia

Comparative Metabolic Characterization of Extraintestinal Pathogenic Escherichia coli Blood Isolates from Saudi Arabia

Plasmid Replicon Diversity of Clinical Uropathogenic Escherichia coli Isolates from Riyadh, Saudi Arabia

Comparative genomics and phenotypic studies to determine site-specificity of Escherichia coli in the lower gastrointestinal tract of humans

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