Comparative pharmacokinetics of six bioactive components of Shen-Wu-Yi-Shen tablets in normal and chronic renal failure rats based on UPLC-TSQ-MS/MS

Mei, Yudan; Tong, Xiaoyu; Hu, Yumei; Liu, Wenjun; Wang, Jiajia; Lv, Kuznetzova; Xu, Liang; Cao, Lei; Wang, Zhenzhong; Gao, Xia; Xiao, Wei

doi:10.1016/j.jep.2023.116818

Cited by 2 publications

(2 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The C max and AUCs increased with dosages. The rest of the compounds were non-linear, which could be attributed to the first-pass effect, absorption, distribution, metabolism, the excretion effect (ADME), and biotransformation [ 14 , 15 , 16 ]. Among them, there was a correlation between CL, AUCs (AUC (0–t) , AUC (0–∞) ), t ½ , and dose for Cynaroside.…”

Section: Resultsmentioning

confidence: 99%

“…indicated the pharmacokinetic characteristics in normal and chronic renal failure exhibited different pharmacokinetic properties [ 34 ]. The results of comparative pharmacokinetics of Enmein, Epinodosin, and Isodocarpin indicated that three diterpenoids of Rabdosia serra Extract were significantly different between control and liver injury rats, which elucidated evident differences in the pharmacokinetic behaviors of compounds between the physiological and pathological states [ 16 ]. Moreover, the results of the pharmacokinetics of Xuanfei Baidu granules showed that pathological state promoted the absorption of several bioactive compounds, and pharmacokinetic behaviors changed in the ARDS rats model [ 35 , 36 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A Pharmacokinetic Study of Sixteen Major Bioactive Components of Jinshui-Huanxian Granules in Pulmonary Fibrosis Model and Control Rats Using Orbitrap Fusion Mass Spectrometry

Zhang,

Wan,

Sun

et al. 2023

Molecules

View full text Add to dashboard Cite

Jinshui-Huanxian granules (JHGs), a Chinese herbal compound prescription, have shown a therapeutic effect in reducing lung tissue damage, improving the degree of pulmonary fibrosis, replenishing lungs and kidneys, relieving cough and asthma, reducing phlegm, and activating blood circulation. However, these active compounds’ pharmacokinetics and metabolic processes were unclear. This study aimed to compare the pharmacokinetics, reveal the metabolic dynamic changes, and obtain the basic pharmacokinetic parameters of 16 main bioactive compounds after intragastric administration of JHGs in control and pulmonary fibrosis (PF) model rats by using Orbitrap Fusion MS. After administration of JHGs, the rat plasma was collected at different times. Pretreating the plasma sample with methanol and internal standard (IS) solution carbamazepine (CBZ), and it was then applied to a C18 column by setting gradient elution with a mobile phase consisting of methanol 0.1% formic acid aqueous solution. Detection was performed on an electrospray ionization source (ESI), and the scanning mode was SIM. Pharmacokinetic parameters were analyzed according to the different analytes’ concentrations in plasma. The matrix effect was within the range of 79.01–110.90%, the extraction recovery rate was 80.37–102.72%, the intra-day and inter-day precision relative standard deviation (RSD) was less than 7.76%, and the stability was good, which met the requirements of biological sample testing. The method was validated (r ≥ 0.9955) and applied to compare the pharmacokinetic profiles of the control group and PF model group after intragastric administration of the JHGs. The 16 analytes exhibited different pharmacokinetic behaviors in vivo. In the pathological state of the PF model, most of the components were more favorable for metabolism and absorption, and it was more meaningful to study the pharmacokinetics. Above all, this study provided an essential reference for exploring the mechanism of action of JHGs and guided clinical medication as well.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A Pharmacokinetic Study of Sixteen Major Bioactive Components of Jinshui-Huanxian Granules in Pulmonary Fibrosis Model and Control Rats Using Orbitrap Fusion Mass Spectrometry

Zhang,

Wan,

Sun

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

Study on the Chemical Composition and Blood-Entry Components of Nourishing Blood Diuretic Formula Based on Liquid Chromatography-Mass Spectrometry and Network Pharmacology Techniques

Yang,

Lei,

Xie

et al. 2024

Natural Product Communications

View full text Add to dashboard Cite

Objective: Analyzing the chemical composition and blood-entry components of Nourishing Blood Diuretic Formula (NBDF) by Liquid Chromatography-Mass Spectrometry (LC-MS), and analyzing the mechanism of NBDF in the treatment of chronic renal failure by combining network pharmacology and molecular docking technology. Methods: The prototype components were obtained by LC-MS, and the targets of the prototype components and the targets for the treatment of chronic renal failure (CRF) were predicted by network pharmacology to obtain the common targets of the drug and the disease, which were then subjected to gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, and molecular docking was carried out between the compounds of the prototype components and the key targets, so as to screen out the active ingredients, the targets and the signaling pathways that were closely related to the efficacy of NBDF. Results: Synthesizing the test information, 125 compounds were identified from the solution of NBDF, and 48 blood-entry components were identified from rat plasma containing the drug, including 6 prototypical components, and 102 potential targets of action, 515 GO entries, and 133 KEGG pathways were obtained from the web-based pharmacological analyses, and molecular docking was performed to obtain the binding of the 6 prototypical component compounds and the 9 key targets, and the formononetin, emodin, epicatechin, chlorogenic acid, sennoside A, and astragaloside III were hypothesized to be the active components of NBDF in the treatment of CRF. Conclusion: This study initially demonstrated that Nourishing Blood and Diuretic Formula exert its therapeutic effects on CRF through multicomponents, multitargets, and multimethods, and elucidated its pharmacological material basis and therapeutic mechanism.

show abstract

Comparative pharmacokinetics of six bioactive components of Shen-Wu-Yi-Shen tablets in normal and chronic renal failure rats based on UPLC-TSQ-MS/MS

Cited by 2 publications

References 64 publications

A Pharmacokinetic Study of Sixteen Major Bioactive Components of Jinshui-Huanxian Granules in Pulmonary Fibrosis Model and Control Rats Using Orbitrap Fusion Mass Spectrometry

A Pharmacokinetic Study of Sixteen Major Bioactive Components of Jinshui-Huanxian Granules in Pulmonary Fibrosis Model and Control Rats Using Orbitrap Fusion Mass Spectrometry

Study on the Chemical Composition and Blood-Entry Components of Nourishing Blood Diuretic Formula Based on Liquid Chromatography-Mass Spectrometry and Network Pharmacology Techniques

Contact Info

Product

Resources

About