Comparative pharmacokinetics of baicalin and wogonoside by liquid chromatography–mass spectrometry after oral administration of Xiaochaihu Tang and Radix scutellariae extract to rats

Zhu, Zhenyu; Zhao, Liang; Liu, Xiaofan; Chen, Jun; Zhang, Hai; Chai, Yifeng

doi:10.1016/j.jchromb.2010.06.021

Cited by 77 publications

(45 citation statements)

References 32 publications

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“…A bimodal phenomenon of 10 flavonoids was presented in Fig. 4, which was in agreement with the literature and was probably due to distribution, re-absorption and enterohepatic circulation (Kowalczyk et al, 2006;Kotani et al, 2006;Zhu et al, 2010;Tong et al, 2012;Kim et al, 2006;Walle et al, 2001;Lu et al, 2007). This is the first study on the pharmacokinetics of flavonoids, including scutellarin, scutellarein and apigenin from RS.…”

Section: Analytessupporting

confidence: 86%

“…To study the pharmacokinetics of these flavonoids in vivo, HPLC-UV (Lu et al, 2007), HPLC-ECD (Kotani et al, 2006) and LC-MS (Zhu et al, 2010) analytical methods have been established. In the last 10 years, large numbers of studies have focused mainly on the pharmacokinetics of baicalin, wogonoside, oroxylin A-7-glucuronide, baicalein, wogonin and oroxylin A in crude RS, single compenents or multiple compenents, five or six at most (Ye et al, 2010;Shi et al, 2011;Tong et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Cui

Qian

Huang

et al. 2014

Biomedical Chromatography

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Radix Scutellariae (RS) is a herbal medicine with various pharmacological activities to treat inflammation, respiratory and gastrointestinal infections, etc. In this study, a rapid, sensitive and selective UPLC-ESI-MS/MS method was developed for simultaneous determination of 10 flavonoids - scutellarin, scutellarein, chrysin, wogonin, baicalein, apigenin, wogonoside, oroxylin A-7-O-glucuronide, oroxylin A and baicalin - from RS aqueous extracts in rat plasma with propyl paraben as internal standard (IS). Chromatographic separation was achieved on a C18 column using gradient elution with the mobile phase consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. The detection was performed in multiple reaction monitoring mode using electrospray ionization in negative mode. The validated method showed good linearity over a wide concentration range (r >0.9935). The intra- and interday assay variabilities were <9.5% and <12.4% for all analytes, respectively. The extraction recovery ranged from 71.2 to 89.7% for each analyte and IS. This method was successfully applied to pharmacokinetic comparision after oral administration of crude and wine-processed RS aqueous extracts. There were significant differences in some pharmacokinetic parameters of most analytes between crude and wine-processed RS. This suggested that wine-processing exerted effects absorption of most flavonoids.

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Section: Analytessupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Cui

Qian

Huang

et al. 2014

Biomedical Chromatography

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“…Cinnamaldehyde reaching its maximum level at approximately 1 h (22.7 ng/mL), and then it showed prolonged elimination (T 1/2 = 8.70 h), and it could be detected in rat whole blood for at least 24 h. In Figure 3, a double peak phenomenon was observed, some similar reports also supported this finding [21][22]. This phenomenon may cause by enterohepatic circulation, fractionated gastric emptying, and separated "absorption windows" [23][24][25][26]. Further studies should be carried out to confirm which mechanisms it was.…”

Section: Pharmacokinetic Applicationsupporting

confidence: 57%

Simultaneous determination of cinnamaldehyde, cinnamic acid, and 2-methoxy cinnamic acid in rat whole blood after oral administration of volatile oil of Cinnamoni Ramulus by UHPLC-MS/MS: An application for a pharmacokinetic study

Zhao

Zhang

Wang

et al. 2015

Journal of Chromatography B

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“…Therefore, BG has been used as a phytochemical marker for the quality control of RS and YHG in the Chinese Pharmacopoeia (National Pharmacopoeia Committee, 2010). In fact, the pharmacokinetic interaction of co-existing ingredients from RS or other herbs has been verified by previous studies (Lu et al, 2007;Shi et al, 2009;Zhu et al, 2010). In fact, the pharmacokinetic interaction of co-existing ingredients from RS or other herbs has been verified by previous studies (Lu et al, 2007;Shi et al, 2009;Zhu et al, 2010).…”

Section: Introductionmentioning

confidence: 54%

Simultaneous determination of baicalin, oroxylin A‐7‐O‐glucuronide and wogonoside in rat plasma by UPLC‐DAD and its application in pharmacokinetics of pure baicalin, Radix Scutellariae and Yinhuang granule

Chen

et al. 2015

Biomedical Chromatography

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A novel UPLC-DAD method was developed and validated for the simultaneous determination of baicalin (baicalein-7-glucuronide, BG), oroxylin A-7-O-glucuronide (OAG) and wogonoside (WG) in rat plasma using rutin as the internal standard. Plasma samples were precipitated using acetonitrile containing 0.1% formic acid. Separation was performed on an Agilent Eclipse Plus C18 column (2.1 × 50 mm, 1.8 µm) using gradient acetonitrile and 0.2% formic acid water solution as mobile phase. The flow-rate was set at 0.4 mL/min and the eluate was detected at 275 nm. The method was linear over the ranges of 0.075-17.50, 0.050-12.60 and 0.056-14.10 µg/mL for BG, OAG and WG, respectively. The intra- and inter-day precisions were respectively <4.8% and 6.4%. All of the limits of detection of three analytes in rat plasma were 0.01 µg/mL, whereas the limits of quantification were, respectively, 0.035, 0.025 and, 0.025 µg/mL. This assay has been successfully applied to pharmacokinetics of BG, OAG and WG in rats after oral administration of Yinhuang granule (YHG) and comparative pharmacokinetics of BG in rats following oral administration of the pure BG, Radix Scutellariae (RS) or YHG. We speculate that some co-existing ingredients in RS or YHG may increase the absorption and elimination of BG in rat. This work may be helpful for the quality control of Yinhuang granule.

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Comparative pharmacokinetics of baicalin and wogonoside by liquid chromatography–mass spectrometry after oral administration of Xiaochaihu Tang and Radix scutellariae extract to rats

Cited by 77 publications

References 32 publications

Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Simultaneous determination of cinnamaldehyde, cinnamic acid, and 2-methoxy cinnamic acid in rat whole blood after oral administration of volatile oil of Cinnamoni Ramulus by UHPLC-MS/MS: An application for a pharmacokinetic study

Simultaneous determination of baicalin, oroxylin A‐7‐O‐glucuronide and wogonoside in rat plasma by UPLC‐DAD and its application in pharmacokinetics of pure baicalin, Radix Scutellariae and Yinhuang granule

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