This paper studies the inheritance and heterotic effect in ten F1 crosses obtained by one-way diallel crossing between five parent genotypes: MV-1, P 76/86, Adiyaman, Basma-Cebel and P 66 9 7, for the following quantitative traits: number of leaves per stalk, length of leaves from the middle belt of the stalk and yield of green leaf mass per stalk and per hectare. The experiment was placed on the experimental field at the Scientific Institute for Tobacco -Prilep by random block system in 4 repetitions in the period from 2018 and 2019. During the vegetation period of the tobacco in the fields, all the usual agro-technical measures were applied. The aim of this work was to study the mode of inheritance of the quantitative traits, to detect heterosis in F1 generation and assess its economic viability. Analysis of variance revealed statistically significant differences between parents and their hybrids for traits in the two years of investigation. The most common way of inheritance of the traits is the partial-dominant, then the intermediate. Negative heterotic effect on the number of leaves per stalk has in P