Comparative Performance of Different Malaria Diagnostic Tools among Pregnant Cohorts in Onitsha Southeast Nigeria

Uba, Christian Chibuzo; Ikegbunam, Moses Nkechukwu; Anagor, Ifeoma Sandra; Eleanya, Lilian Chinyere; Ezeumeh, Eucharia Nkiruka; Ezekwueche, Stephen Nnaemeka; Okechukwu, Edith Chinenye; Esimone, Charles Okechukwu

doi:10.4236/aid.2021.114035

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…The disease is transmitted to humans through the bite of an infected female Anopheles mosquito during a blood meal [3]. Five Plasmodia species, namely Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae and P. knowlesi [4] are the parasites responsible for the actual disease condition with P. falciparum being the most lethal [2,5] and is the most predominant specie in Nigeria and other sub-Saharan African countries [6]. Over half of the world's population lives in malaria-prone areas [7], and according to World Health Organisation (WHO), in 2017 there were about 219 million clinical cases of malaria and about 435,000 malaria deaths in 87 countries in the world, and Nigeria accounts for 25% of these cases [6].…”

Section: Introductionmentioning

confidence: 99%

“…Five Plasmodia species, namely Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae and P. knowlesi [4] are the parasites responsible for the actual disease condition with P. falciparum being the most lethal [2,5] and is the most predominant specie in Nigeria and other sub-Saharan African countries [6]. Over half of the world's population lives in malaria-prone areas [7], and according to World Health Organisation (WHO), in 2017 there were about 219 million clinical cases of malaria and about 435,000 malaria deaths in 87 countries in the world, and Nigeria accounts for 25% of these cases [6]. The disease is a serious public health problem, especially in tropical African countries where transmission of the disease occur more often [8] partly due to favourable climatic condition that favours optimal growth, survival and development of both parasite and vector of the disease.…”

Section: Introductionmentioning

confidence: 99%

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Performance analysis of polymerase chain reaction in relation to rapid diagnostic test (RDT) kit (SD bio line malaria ag p.f (05fk50)) for malaria diagnosis

Muhammad

Sale

Mohammed

et al. 2022

Cell. Mol. Biomed. Rep.

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Malaria control and its elimination heavenly depend on successful and reliable diagnosis using recommended diagnostic techniques. These available techniques often have certain peculiarities and mode applications, thus making them have different levels of performance and accuracy. Therefore the aim of this study was to evaluate the performance of PCR in relation to Rapid Diagnostic Test Kit (SD Bio line Malaria Ag P.f (05fk50)) in malaria diagnosis. A total of 200 blood samples were collected from the consented study subjects using the vein puncture technique and analysed using PCR and RDTs. Plasmodium falcifarum's DNA was extracted using Quick-DNA™ Miniprep Plus Kit with catalog number D4069. 18SrRNA gene of Plasmodium falciparum from chromosome 13 was amplified using the two primers. For the RDTs technique, the SD Bio line Malaria Ag P.f (05fk50) test kit was used. Malaria prevalence of 106(53.0%) and 132(66.0%) were recorded using PCR and RDTs respectively. The PCR demonstrates an overall accuracy of 0.53 with sensitivity and specificity values of 56.06 and 52.94% respectively. The negative and positive predictive values were 69.81 and 38.30% respectively. PCR demonstrated a good level of performance and is therefore recommended as an effective diagnostic tool for malaria, especially in patients where the parasite density/parasitaemia level is very low.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Performance analysis of polymerase chain reaction in relation to rapid diagnostic test (RDT) kit (SD bio line malaria ag p.f (05fk50)) for malaria diagnosis

Muhammad

Sale

Mohammed

et al. 2022

Cell. Mol. Biomed. Rep.

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Prevalence and Molecular Detection of Plasmodium falciparum among Pregnant Women Attending Selected Hospitals in Kaduna North Local Government Area, Kaduna State

Dikwa,

Baban Takko,

Vantsawa

et al. 2023

UJMR

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Pregnancy-related malaria is a serious public health issue that significantly raises the risk of death for both mothers and babies in malaria-endemic nations. This study was carried out to determine the prevalence of malaria and molecularly detect Plasmodium falciparumamong pregnant women attending selected hospitals in Kaduna North Local Government Area, Kaduna State.Blood samples were collected from 309 pregnant women attending BarauDikko Teaching Hospital Kaduna, General Hospital Kawo and Nigerian Defence Academy Hospital Kaduna. The samples were analysed microscopically and molecularly for Plasmodium falciparum. A self-administered questionnaire was administered to each pregnant woman to obtain information such as occupation, age and gestation period. Results show a prevalence of 65.0% forPlasmodium falciparum malaria among the pregnant women with significant (p < 0.05) difference between malaria and sampling location. The highest prevalence was recorded in pregnant women attending General Hospital Kawo with a prevalence of 76.9%, followed by women attending NDA hospital with a prevalence of 65.0% and those attending Barau Dikko Teaching Hospital had 53.4%. %). Prevalence of Plasmodium falciparum was seen higher in women in their second trimester of pregnancy with 68.9% prevalence, younger aged pregnant women with 66.7% prevalence and self-employed pregnant women with 66.2% prevalence. Results further showed that out of 12 samples with high parasite density subjected to molecular analysis, only 5 (41.7%) were positive for Plasmodium falciparum. The study revealed the prevalence of 65.0% of P falciparum infection among pregnant women attending selected hospitals in the studied area. Adequate utilization of insecticide treated net is essential to prevent exposure to mosquito bites leading to reduction in maternal mortality.

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Comparative Performance of Different Malaria Diagnostic Tools among Pregnant Cohorts in Onitsha Southeast Nigeria

Cited by 2 publications

References 26 publications

Performance analysis of polymerase chain reaction in relation to rapid diagnostic test (RDT) kit (SD bio line malaria ag p.f (05fk50)) for malaria diagnosis

Performance analysis of polymerase chain reaction in relation to rapid diagnostic test (RDT) kit (SD bio line malaria ag p.f (05fk50)) for malaria diagnosis

Prevalence and Molecular Detection of Plasmodium falciparum among Pregnant Women Attending Selected Hospitals in Kaduna North Local Government Area, Kaduna State

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