Comparative Performance of Different HPLC Systems with Electrochemical Detection

Perrett, David

doi:10.1007/978-1-4613-2397-6_14

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…For maximum sensitivity, it is more important to work at optimal signal-to-noise ratio than at maximum peak height. 26 This value is often about 0·05 V below the signal maximum. In practice, the digital filtering by the integrator does allow the attainment of increased sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Methodological Considerations in the Determination of Plasma Catecholamines by High-Performance Liquid Chromatography with Electrochemical Detection

1985

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We report on a systematic investigation into some frequently encountered problems in the estimation of catecholamines in plasma and biological fluids using high-performance liquid chromatography with electrochemical detection. The kinetics of adsorption and desorption of catecholamines from plasma on to alumina has been studied using laboratory-prepared and some commercial aluminas with various acids. Chromatographic conditions yielding optimal resolution and sensitivity have been characterised. Some possible interfering peaks have been identified. The stability of catecholamines in plasma has been studied.

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Section: Discussionmentioning

confidence: 99%

Methodological Considerations in the Determination of Plasma Catecholamines by High-Performance Liquid Chromatography with Electrochemical Detection

1985

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The determination of cysteamine in physiological fluids by HPLC with electrochemical detection

Perrett

Rudge

1987

Biomedical Chromatography

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Cysteamine, an amino thiol, was separated by rapid isocratic cation exchange chromatography and detected by electrochemical oxidation at a platinum electrode maintained at +0.45 V relative to an Ag/AgCl reference electrode. Eluent pH and electrode working potentials were optimized and the effects of alternative buffers and organic modifiers have been examined. On column sensitivity for cysteamine was 1.5 pmol at a signal-to-noise ratio of 5. Although the specificity was good, plasma samples required maximal sensitivity whereas urine samples required greater selectivity, which was achieved by use of lower working potentials. Cysteamine concentrations were determined in serial samples of plasma and urine from volunteers who had received a single oral dose of 200 mg of the drug. Cysteamine was rapidly oxidized in vivo, and detection required prior reduction with dithiothreitol before analysis.

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Comparative Performance of Different HPLC Systems with Electrochemical Detection

Cited by 2 publications

References 12 publications

Methodological Considerations in the Determination of Plasma Catecholamines by High-Performance Liquid Chromatography with Electrochemical Detection

Methodological Considerations in the Determination of Plasma Catecholamines by High-Performance Liquid Chromatography with Electrochemical Detection

The determination of cysteamine in physiological fluids by HPLC with electrochemical detection

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