Comparative performance of airyscan and structured illumination superresolution microscopy in the study of the surface texture and 3D shape of pollen

Sivaguru, Mayandi; Urban, Michael A.; Fried, Glenn; Wesseln, Cassandra J.; Mander, Luke; Punyasena, Surangi W.

doi:10.1002/jemt.22732

Cited by 65 publications

(55 citation statements)

References 37 publications

(42 reference statements)

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“…Some other pollen types were earlier studied with CLSM, such as the commonest 3‐colpate and 3‐colporate pollen types (Liu et al. , ; Castro et al ., ; Gavrilova, , ,b; Sivaguru et al ., , ; Mander & Punyacena, ), monoporate poaceous pollen (Salih et al ., ; Sivaguru et al ., , ; Mander & Punyacena, ), sulcate pollen (Castro et al ., ), porate pollen and pollen amassed in polyads (Gavrilova, ), and the Croton‐ pollen type (Sivaguru et al ., , ). Saccate and nonsaccate types were studied among pollen grains of modern gymnosperms (Castro et al ., ; Gavrilova, ; Mander & Punyacena, ).…”

Section: Application Of Clsm To Palynological Objectsmentioning

confidence: 99%

“…Sivaguru et al . () used CLSM for a comparison with airyscan and structured illumination superresolution microscopy in a study of several modern and fossil pollen types. Hochuli & Feist‐Burkhardt (, ), Zhuo et al .…”

Section: Introductionmentioning

confidence: 99%

“…Modern pollen grains were studied with CLSM by Salih et al (1997), Liu et al (2009), Castro et al (2010), Wang et al (2011), Gavrilova (2012Gavrilova ( , 2014a, Sivaguru et al (2012Sivaguru et al ( , 2016 and Mander & Punyacena (2014). Different tasks were set by these authors such as comparisons of the texture and sculpture of Poaceae pollen (Salih et al, 1997), pollen descriptions in CLSM of different taxa (Castro et al, 2010;Gavrilova, 2012Gavrilova, , 2014bSivaguru et al, 2012), estimation of the volume and density of pollen grains (Golovko et al, 2011), determination of apertural regions in developing tetrads (Banks et al, 2006) and a study of microsporogenesis (Wang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Different tasks were set by these authors such as comparisons of the texture and sculpture of Poaceae pollen (Salih et al, 1997), pollen descriptions in CLSM of different taxa (Castro et al, 2010;Gavrilova, 2012Gavrilova, , 2014bSivaguru et al, 2012), estimation of the volume and density of pollen grains (Golovko et al, 2011), determination of apertural regions in developing tetrads (Banks et al, 2006) and a study of microsporogenesis (Wang et al, 2011). In some studies, pollen grains were prestained by basic fuchsin (Banks et al, 2006), acridine orange (Liu et al, 2009;Wang et al, 2011) and Phloxine B or Periodic AcidSchiff (Sivaguru et al, 2016). Liu et al (2009) and Gavrilova (2012Gavrilova ( , 2014a indicated that CLSM provides more information on the pollen and exine structure by studying optical sections of objects and making reconstructions of whole objects.…”

Section: Introductionmentioning

confidence: 99%

“…Sivaguru et al (2012) made a comparison of different methods in their study of three different pollen types: widefield, apotome, confocal and two-photon microscopy by reflected light techniques, and bright field and differential interference contrast microscopy (DIC) by transmitted light techniques. Sivaguru et al (2016) used CLSM for a comparison with airyscan and structured illumination superresolution microscopy in a study of several modern and fossil pollen types. Hochuli & Feist-Burkhardt (2004, Zhuo et al (2006), Peyrot et al (2007), Zavialova et al (2014a,b), Mander & Punyacena (2014), Tekleva et al (2015), Schopf et al (2016) and applied CLSM for morphological descriptions of fossil pollen and/or spores, and Shute et al (1996) for cryptospores.…”

Section: Introductionmentioning

confidence: 99%

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Potential of CLSM in studying some modern and fossil palynological objects

Гаврилова

Zavialova

Tekleva

et al. 2017

Journal of Microscopy

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We have tested possibilities and limitations of confocal laser scanning microscopy to study the morphology of pollen and spores and inner structure of sporoderms. As test objects, we used pollen grains of the modern angiosperm Ribes niveum (Grossulariaceae) and Datura metel (Solanaceae), fossil angiosperm pollen grains of Pseudointegricorpus clarireticulatum and Wodehouseia spinata dated to the Late Cretaceous, fossil gymnosperm pollen grains of Cycadopites-type dated to the Middle Jurassic, and fossil megaspores Maexisporites rugulaeferus, M. grosstriletus, and Trileites sp. dated to the Early Triassic. For comparative purpose, we studied the same objects with application of conventional light, scanning electron (to entire pollen grains and spores or to semithin sections of their walls), or transmission electron microscopy. The resolution of confocal microscope is much lower than that of electron microscopes, as are its abilities to reconstruct the surface patterns and inner structure. On the other hand, it can provide information that is unreachable by other microscopical methods. Thus, the structure of endoapertures in angiosperm pollen grains can be directly observed. It is also helpful in studies of asymmetrical pollen and pollen grains bearing various appendages and having complicated exine structure, because rotation of 3-D reconstructions allows one to examine all sides and structures of the pollen grain. The exact location of all visible and concealed structures in the sporoderm can be detected; this information helps to describe the morphology and inner structure of pollen grains and to choose necessary directions of further ultrathin sectioning for a transmission electron microscopical study. In studies of fossil pollen grains that are preserved in clumps and stuck to cuticles, confocal microscope is useful in determining the number of apertures in individual pollen grains. This can be done by means of virtual sections through 3-D reconstructions of pollen grains. Fossil megaspores are too large and too thick-walled objects for a confocal study; however, confocal microscope was able to reveal a degree of compression of fossil megaspores, the presence of a cavity between the outer and inner sporoderm layers, and to get some information about sporoderm inner structure.

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Section: Application Of Clsm To Palynological Objectsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Potential of CLSM in studying some modern and fossil palynological objects

Гаврилова

Zavialova

Tekleva

et al. 2017

Journal of Microscopy

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Monitoring of intracellular localization of Hepatitis B virus P22 protein using Laser Scanning Confocal Microscopy and Airyscan

Deroubaix

Moahla

Penny

2020

Microscopy Res & Technique

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The aim of this study was to assess nucleo-cytoplasmic protein localization to better understand the exact intracellular localization of viral proteins involved with infections. Having determined the general protein localization of hepatitis B virus P22 precore protein, the aim was to more specifically resolve its intracellular organization.This was done using both laser scanning microscopy and Airyscan techniques. Using a 63× objective, the resolution obtained with Airyscan was increased by 1.5-fold as compared to confocal microscopy (p value <.00001). K E Y W O R D SAiryscan, confocal, half width full maximum, hepatitis B P22, line profile analysis, resolution

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A simple, inexpensive and multi‐scale 3‐D fluorescent test sample for optical sectioning microscopies

Olevsko

Szederkenyi

Corridon

et al. 2021

Microscopy Res & Technique

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Fluorescence standards allow for quality control and for the comparison of data sets across instruments and laboratories in applications of quantitative fluorescence. For example, users of microscopy core facilities can expect a homogenous and time‐invariant illumination and an uniform detection sensitivity, which are prerequisites for imaging analysis, tracking or fluorimetric pH or Ca2+‐concentration measurements. Similarly, confirming the three‐dimensional (3‐D) resolution of optical sectioning microscopes calls for a regular calibration with a standardized point source. The test samples required for such measurements are typically different ones, they are often expensive and they depend much on the very microscope technique used. Similarly, the ever‐increasing choice among microscope techniques and geometries increases the demand for comparison across instruments. Here, we advocate and demonstrate the multiple uses of a surprisingly versatile and simple 3‐D test sample that can complement existing and much more expensive calibration samples: commercial tissue paper labeled with a fluorescent highlighter pen. We provide relevant sample characteristics and show examples ranging from the sub‐μm to cm scale, acquired on epifluorescence, confocal, image scanning, two‐photon (2P) and light‐sheet microscopes.

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Comparative performance of airyscan and structured illumination superresolution microscopy in the study of the surface texture and 3D shape of pollen

Cited by 65 publications

References 37 publications

Potential of CLSM in studying some modern and fossil palynological objects

Potential of CLSM in studying some modern and fossil palynological objects

Monitoring of intracellular localization of Hepatitis B virus P22 protein using Laser Scanning Confocal Microscopy and Airyscan

A simple, inexpensive and multi‐scale 3‐D fluorescent test sample for optical sectioning microscopies

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