Comparative ontogenesis of Coffea arabica L. somatic embryos reveals the efficiency of regeneration modulated by the explant source and the embryogenesis pathway

Ferrari, Ilse Fernanda; Marques, Giovanna Arcolini; Sachetti, Welington Luis; Biazotti, Bárbara Bort; Passos, Matheus Pena; Almeida, Julieta Andréa Silva de; Mondego, Jorge Maurı́cio Costa; Mayer, Juliana Lischka Sampaio

doi:10.1007/s11627-021-10200-5

Cited by 1 publication

(2 citation statements)

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“…For example, in the woody monocot Phoenix dactylifera, calli are formed from fascicular parenchyma cells or perivascular sheath cells (PSCs) depending on the differentiation grade of the leaf explant used 48 . In Coffea arabica , a pro-embryogenic mass and callus originate from mitotically active cells found near vascular bundles 49 . Similarly in our study, Sansevieria leaf calli seem to derive exclusively from PSCs or totipotent cells surrounding fibers (sclerenchyma).…”

Section: Resultsmentioning

confidence: 99%

“…Snake plants are also recognized for their rapid root production during conventional propagation using rhizomes or adult leaf cuttings 25 , 49 , 61 , suggesting that levels of endogenous auxins are sufficient for rhizogenesis. During the development of our protocol for in vitro multiplication, we observed that leaf explants on basal 1X MS medium yielded a higher frequency of root induction from Hahnii (63.3%) compared to Lorentii (12.2%) (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production

García-Hernández

Loera-Quezada

Morán-Velázquez

et al. 2022

Sci Rep

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Sansevieria trifasciata is used as an indoor plant, in traditional medicine and as a fiber source. Here we characterized fibers of two of varieties of S. trifasciata, Lorentii and Hahnii, and report a protocol for their propagation based on indirect shoot organogenesis. Structural and ribbon fibers were scattered within leaf parenchyma when viewed with confocal laser scanning microscopy. Chemical analysis of the fibers by mass spectrometry and high-performance chromatography revealed higher contents of cellulose and xylose in Lorentii than in Hahnii and significant differences for total lignin between both. A protocol for de novo shoot production was then developed using leaf explants. Time-course histological analyses showed that the first events of transdifferentiation were triggered preferentially in cells surrounding fibers and vascular bundles. Callogenesis and shoot performances were quantified for both varieties, and 2,4-D at 2 and 3 mg·L-1 yielded the best results for primary calli induction and fresh calli mass. The length, number, and mass of shoots produced did not differ significantly between the two cultivars. The fast morphogenic response of S. trifasciata to in vitro culture may be useful for mass propagation or other biotechnological purposes such as metabolite production.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%