Comparative observations of the growth changes of the histochemical properties and collagen architecture of the iliotibialis lateralis muscle from Silky, layer and meat type cockerels

Abstract

Growth-related changes in the histochemical property and collagen architecture of the iliotibialis lateralis muscle were compared among Silky, layer and meat cockerels. Histochemical and immunohistochemical methods were employed to observe the collagen architecture. The total intramuscular collagen was also determined. The muscle consisted of type IIA, IIB and IIC myofibers, of which type IIB occurred at the highest frequency. The diameter of type IIB myofibers in each week was largest in the layer, followed by the meat, and was smallest in the Silky. The total amount of collagen reached 3.38 mg/g in the meat bird, 3.03 mg/g in the layer and 2.71 mg/g in the Silky by 30 weeks of age, respectively. In the perimysium, the collagen bundles increased in size and density of fibrils with growth. At 30 weeks of age the layer had compact collagen platelets while the Silky had loose collagen bundles. In the meat bird, the collagen bundles were moderately compact. The endomysial collagen network had a large mesh size at 1 week and thereafter accumulated many collagen fibrils to form a felt-like fabric of fibrils at 30 weeks of age. From these results it appears that growth-related changes in the iliotibialis lateralis muscle are not necessarily causally affected by the different growth rates of chicken breeds.

Collagen content and architecture of the pectoralis muscle in male chicks and broilers reared under various nutritional conditions

“…This growth‐related change of endomysial thickness has already been reported in chickens (Nakamura et al. 2004; Oshima et al. 2007a,b),wild boars and domestic pigs (Fang et al.…”

“…Because the collagen concentration remains within a relatively narrow range throughout the different growth stages (Nakamura et al. 2004; Oshima et al. 2007a,b), inconsistent results may be obtained depending on the experimental conditions.…”

Comparative observation on the growth changes of the histochemical properties and collagen architecture of the Musculus puboischiofemoralis pars medialis from Silky, layer‐type and meat‐type cockerels

“…Because of the small size of the muscle under study it was difficult to collect sufficient material for determination of collagen content (Table 1). Although the total amount of collagen in layer‐ and meat‐type chickens were analyzed in the whole muscle, the values, 2.10–3.58 mg/g at 20 and 30 weeks of age (Table 3), were similar to the results found for the typical fast‐twitch pectoralis (2.2–2.5 mg/g) and M. iliotibialis lateralis (2.04–3.38 mg/g) using materials which did not include the epimysium (Oshima et al . 2006a,b).…”

“…(Photographs of type III collagen detected immunohistochemically are not shown.) In all breeds at 30 weeks of age, the positive endomysial networks were limited by thin or thick perimysia into primary and secondary myofiber fascicles, of which the sizes were small compared with the fascicles of the M. pectoralis and M. iliotibialis lateralis (Oshima et al . 2006a,b).…”

“…5a–c). However, the endomysial collagen showed a more developed state compared with that of the M. pectoralis and M. iliotibialis lateralis (Oshima et al . 2006a,b).…”