Comparative observation of fibroblasts derived from the papillary and reticular dermis of infants and adults: Growth kinetics, packing density at confluence and surface morphology

Fibroblast heterogeneity: implications for human disease

“…Papillary and reticular fibroblasts can be isolated by explant culture of mechanically sectioned (dermatomed) human dermis. Explant fibroblast cultures derived from these layers exhibit differences in a number of parameters, including rate of cell division, contraction, and the expression of various collagens and proteoglycans (5,51,52). Cultured fibroblasts derived from the papillary dermis support the formation of a normal stratified epidermis in three-dimensional organotypic culture more effectively than fibroblasts from the reticular dermis (47,53,54).…”

Development, validation and testing of a human tissue engineered hypertrophic scar model

“…In short, split thickness skin (0.4 mm) was removed using a dermatome (Acculan II, Braun, tuttligen, Germany) to separate the papillary dermis from reticular dermis and adipose tissue (Schafer et al, 1985). the cells in the papillary dermis (upper layer) are further referred as P-DSC.…”

Fibroblast heterogeneity: more than skin deep

“…Reticular dermal fibroblasts seeded into type I collagen lattices contract them faster than do papillary dermal fibroblasts (Schafer et al, 1985;Sorrell et al, 1996). When grown to confluence in monolayer culture, the papillary cells attain a higher cell density partly because they are not fully contact inhibited (Schafer et al, 1985;Sorrell et al, 2004).…”

“…Papillary fibroblasts divide at faster rates than do site-matched reticular fibroblasts (Harper and Grove, 1979;Azzerone and Macieira-Coelho, 1982;Schafer et al, 1985;Sorrell et al, 1996;Sorrell et al, 2004). Reticular dermal fibroblasts seeded into type I collagen lattices contract them faster than do papillary dermal fibroblasts (Schafer et al, 1985;Sorrell et al, 1996).…”