Comparative morphology, biology and histology of reproductive development in three lines of Manihot esculenta Crantz (Euphorbiaceae: Crotonoideae)

Perera, P. I. P.; Quintero, Marcela; Dedičová, Beata; Kularatne, J.D.J.S.; Ceballos, Hernán

doi:10.1093/aobpla/pls046

Cited by 35 publications

(56 citation statements)

References 34 publications

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“…Important advances have been made on the reproductive biology of cassava in recent years [18,19]. Nonetheless, there are still important features of the sexual reproduction in cassava that remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Reproductive biology in cassava: stigma receptivity and pollen tube growth

Abril

Pineda

Wąsek

et al. 2019

Communicative & Integrative Biology

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Knowledge on the reproductive biology of cassava, relevant to breeders and molecular geneticists, is still limited. Therefore, different studies were carried out to determine the duration of stigma receptivity and the rate of pollen tube growth. Inflorescences were covered for up to 3 days after the first opening of the bracts (e.g. anthesis day) to prevent open pollination. Results indicate that fruit and seed set are drastically reduced when flowers were covered for 2 or 3 days. However, fruits and seeds were obtained even from flowers that had been covered for 3 days after anthesis, although at low frequency. The rate of pollen tube growth was assessed in many combinations of female and male progenitors crossed through controlled pollinations and collecting the pistils at varying hours after pollination (HAP). Pollen tube growth is fast during the first 6 HAP reaching the tip of the nucellar beak. The growth slows down thereafter, taking 10 additional hours to reach the end of the beak. The growth of pollen tubes slows down even further until they enter the embryo sac. Only 10% of samples showed pollen tubes entering the embryo sac between 48 and 66 HAP. Although several tubes may reach the nucellar beak, only one was observed entering the embryo sac. Results, across the different experiments, were highly variable suggesting that the timeline of fertilization is influenced both by genotypic and environmental factors as well as the manual manipulation of inflorescences and cyathia.

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Section: Introductionmentioning

confidence: 99%

Reproductive biology in cassava: stigma receptivity and pollen tube growth

Abril

Pineda

Wąsek

et al. 2019

Communicative & Integrative Biology

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“…10D). Nuclear fusion may happen within these syncytial meiocytes and may develop into unreduced microspores as reported in cassava by Perera et al (2013). Reprogramming in such unreduced microspores may give rise to MCSs and heterozygous diploid plants.…”

Section: Discussionmentioning

confidence: 81%

Reprogramming of cassava (Manihot esculenta) microspores towards sporophytic development

et al. 2014

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Gametes have a unique potential to enter the sporophytic pathway, called androgenesis. The plants produced are usually haploid and recombinant due to the preceding meiosis and they can double their chromosome number to form doubled haploids (DHs), which are completely homozygous. The present study demonstrates that reprogramming occurs in the isolated microspores at tetrad stage as the first step if androgenesis and this is paving the way for the development of an efficient technique for the production of homozygous lines in cassava. This is the first ever detailed report of microspore reprogramming at the tetrad stage and the first report of microspore embryogenesis induction in cassava with detailed evidence.

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“…4a) as described by Perera et al (2013). The wall cells of cultured anthers, epidermis, endothecium, and middle layer were enlarged (Fig.…”

Section: Resultsmentioning

confidence: 99%

A milestone in the doubled haploid pathway of cassava

et al. 2013

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This study was aimed at inducing androgenesis in cultured anthers of cassava (Manihot esculenta Crantz) to develop a protocol for the production of doubled haploids. Microspore reprogramming was induced in cassava by cold or heat stress of anthers. Since the anthers contain both haploid microspores and diploid somatic cells, it was essential to verify the origin of anther-derived calli. The origin of anther-derived calli was assessed by morphological screening followed by histological analysis and flow cytometry (FCM). Additionally, simple sequence repeat (SSR) and amplified fragmented length polymorphism (AFLP) assays were used for the molecular identification of the microspore-derived calli. The study clearly demonstrated the feasibility of producing microspore-derived calli using heat- or cold-pretreated anthers. Histological studies revealed reprogramming of the developmental pathway of microspores by symmetrical division of the nucleus. Flow cytometry analysis revealed different ploidy level cell types including haploids, which confirmed their origin from the microspores. The SSR and AFLP marker assays independently confirmed the histological and FCM results of a haploid origin of the calli at the DNA level. The presence of multicellular microspores in the in vitro system indicated a switch of developmental program, which constitutes a crucial step in the design of protocols for the regeneration of microspore-derived embryos and plants. This is the first detailed report of calli, embryos, and abnormal shoots originated from the haploid cells in cassava, leading to the development of a protocol for the production of doubled haploid plants in cassava.

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Comparative morphology, biology and histology of reproductive development in three lines of Manihot esculenta Crantz (Euphorbiaceae: Crotonoideae)

Cited by 35 publications

References 34 publications

Reproductive biology in cassava: stigma receptivity and pollen tube growth

Reproductive biology in cassava: stigma receptivity and pollen tube growth

Reprogramming of cassava (Manihot esculenta) microspores towards sporophytic development

A milestone in the doubled haploid pathway of cassava

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