Comparative molecular portraits of human unfertilized oocytes and primordial germ cells at 10 weeks of gestation

Diedrichs, Ferdinand; Mlody, Barbara; Matz, Peggy; Fuchs, Heiko; Chávez, Lukas; Drews, Katharina; Adjaye, James

doi:10.1387/ijdb.120230ja

Cited by 9 publications

(11 citation statements)

References 46 publications

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“…Genes clustered in this group were: OR51D1 , OR51E1 , OR52B4 , OR52K1 , OR52K2 (BTA15, at 51-52Mb). Two of these, OR51D1 and OR52K1 were found to be expressed in female and male primordial germ cells and in unfertilized human oocytes, participating in gamete production [76]. Furthermore, olfactory receptors integrate many metabolic processes and some of them play important role in reproduction [76].…”

Section: Resultsmentioning

confidence: 99%

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Genome-wide association study provides strong evidence of genes affecting the reproductive performance of Nellore beef cows

et al. 2017

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Reproductive traits are economically important for beef cattle production; however, these traits are still a bottleneck in indicine cattle since these animals typically reach puberty at older ages when compared to taurine breeds. In addition, reproductive traits are complex phenotypes, i.e., they are controlled by both the environment and many small-effect genes involved in different pathways. In this study, we conducted genome-wide association study (GWAS) and functional analyses to identify important genes and pathways associated with heifer rebreeding (HR) and with the number of calvings at 53 months of age (NC53) in Nellore cows. A total of 142,878 and 244,311 phenotypes for HR and NC53, respectively, and 2,925 animals genotyped with the Illumina Bovine HD panel (Illumina®, San Diego, CA, USA) were used in GWAS applying the weighted single-step GBLUP (WssGBLUP) method. Several genes associated with reproductive events were detected in the 20 most important 1Mb windows for both traits. Significant pathways for HR and NC53 were associated with lipid metabolism and immune processes, respectively. MHC class II genes, detected on chromosome 23 (window 25-26Mb) for NC53, were significantly associated with pregnancy success of Nellore cows. These genes have been proved previously to be associated with reproductive traits such as mate choice in other breeds and species. Our results suggest that genes associated with the reproductive traits HR and NC53 may be involved in embryo development in mammalian species. Furthermore, some genes associated with mate choice may affect pregnancy success in Nellore cattle.

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Section: Resultsmentioning

confidence: 99%

“…Moreover, MHC-based mate choice continues during sperm-oocyte interaction (chemotaxis) after copulation and is mediated by olfactory receptors present on the gametes [76]. The olfactory receptors of the gametes “reflect” the MHC variability of the oocyte/sperm and guide fecundation between the genetically most distant gametes.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide association study provides strong evidence of genes affecting the reproductive performance of Nellore beef cows

et al. 2017

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“…Some of the genes found in these regions are important because they act as olfactory receptors, including the genes of window Chr07/41.29–42.03Mb ( LOC618125 , LOC614895 , LOC530231 , OR2V1 , LOC100299289 , OR2V2 , and LOC508420 ), in addition to LOC100139608 located in window Chr21/0.01–3.02Mb. A physiological explanation exists for the association of these genes with EP, since olfactory receptors have a function in germ cells, which are the precursors of gametes [ 26 ], affecting their production and consequent reproduction. Olfactory receptors are also expressed in the gametes themselves where they play a role in chemotaxis between the sperm and egg to promote fertilization of the gametes whose major histocompatibility complex (MHC) genes are the most diverse [ 27 ], guaranteeing the success of fertilization.…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Association Study for Indicator Traits of Sexual Precocity in Nellore Cattle

et al. 2016

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The objective of this study was to perform a genome-wide association study (GWAS) to detect chromosome regions associated with indicator traits of sexual precocity in Nellore cattle. Data from Nellore animals belonging to farms which participate in the DeltaGen® and Paint® animal breeding programs, were used. The traits used in this study were the occurrence of early pregnancy (EP) and scrotal circumference (SC). Data from 72,675 females and 83,911 males with phenotypes were used; of these, 1,770 females and 1,680 males were genotyped. The SNP effects were estimated with a single-step procedure (WssGBLUP) and the observed phenotypes were used as dependent variables. All animals with available genotypes and phenotypes, in addition to those with only phenotypic information, were used. A single-trait animal model was applied to predict breeding values and the solutions of SNP effects were obtained from these breeding values. The results of GWAS are reported as the proportion of variance explained by windows with 150 adjacent SNPs. The 10 windows that explained the highest proportion of variance were identified. The results of this study indicate the polygenic nature of EP and SC, demonstrating that the indicator traits of sexual precocity studied here are probably controlled by many genes, including some of moderate effect. The 10 windows with large effects obtained for EP are located on chromosomes 5, 6, 7, 14, 18, 21 and 27, and together explained 7.91% of the total genetic variance. For SC, these windows are located on chromosomes 4, 8, 11, 13, 14, 19, 22 and 23, explaining 6.78% of total variance. GWAS permitted to identify chromosome regions associated with EP and SC. The identification of these regions contributes to a better understanding and evaluation of these traits, and permits to indicate candidate genes for future investigation of causal mutations.

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“…Physiological responses mediated by these chemoreceptors exert a crucial role in animal's appetite and energy balance regulation, which could affect the feed intake, weight gain, and animal body composition 42 . Olfactory receptors also affect the reproduction in mammals by playing a role in production of germ cells, which are the precursors of gametes 43 . According to 44 , the OR2AP1 gene, identified in this study, is associated with fertility and semen quality of zebu cattle under heat stress conditions.…”

Section: Resultsmentioning

confidence: 99%

Whole-genome sequencing provides new insights into genetic mechanisms of tropical adaptation in Nellore (Bos primigenius indicus)

Júnior

Oliveira

Carvalheiro

et al. 2020

Sci Rep

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Most of the knowledge about genetic variants at the sequence level in cattle is for Bos primigenius taurus populations. Here, we presented a complete genomic characterization of 52 Nellore (Bos primigenius indicus) bulls, revealing specific zebu DNA variants with putative impact in tropical adaptation and productive traits. Single nucleotide polymorphisms (Snps) and insertion/deletion (INDELs) mutations were identified using the newest bovine reference genome ARS_UCD1.2, and variant functional consequences were predicted using the Ensembl VEP software. A total of 35,753,707 SNPs and 4,492,636 INDELs were detected and annotated to their functional effects. We identified 400 genes that comprised both, a Snp and an inDeL, of high functional impact on proteins (i.e. variants that cause protein truncation, loss of function or triggering nonsense-mediated decay). Among these, we highlight the following genes: BoLA, associated with cattle immune response to infections and reproduction aspects; HSPA8, DNAJC27, and DNAJC28, involved with thermoregulatory protective mechanisms in mammals; and many olfactory signaling pathway related genes that are important genetic factors in the evolution of mammalian species. All these functional aspects are directly related to cattle adaptability to tropical environments. Cattle have played important roles in human societies for a long time, supplying products such as milk, meat, leather, and power. The current two major domesticated cattle, taurine (Bos primigenius taurus) and indicine or zebu (Bos primigenius indicus), descend from the extinct wild aurochs (Bos primigenius) that have diverged, between 250,000 and 330,000 years ago, into two distinct lineages 1. Taurine cattle originated from the domestication of the Bos primigenius primigenius at, approximately, 10,000 years ago in the Fertile Crescent, while the indicine cattle descendant of the Bos primigenius nomadicus, domesticated about 8,000 years ago in the Indus Valley 1-5. Natural selection, followed by the post-domestication selection driven by humans modified cattle genotypes, leading to distinct genetic and phenotypes profiles between taurine and indicine cattle. In general, these two groups have been selected and are adapted to temperate and tropical environments, respectively 2,4. Since the taurine cattle genome sequencing 6 , an extensive use of re-sequenced animals has allowed the identification of a considerable number of genetic variants segregating in different populations, essentially represented by taurine breeds 7-13. To this point, a few studies have explored sequence information of indicine cattle breeds 14-16. Nellore figures between the most important domestic indicine cattle with a great impact on the global beef industry. It is a tropically adapted breed and the main responsible for transforming Brazil in one of the largest beef producers and exporters of the world 17,18. The Brazilian Nellore originated from Ongole cattle which were brought to Brazil from India between 1868 and 1963 19. Crosses with local ...

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Comparative molecular portraits of human unfertilized oocytes and primordial germ cells at 10 weeks of gestation

Cited by 9 publications

References 46 publications

Genome-wide association study provides strong evidence of genes affecting the reproductive performance of Nellore beef cows

Genome-wide association study provides strong evidence of genes affecting the reproductive performance of Nellore beef cows

Genome-Wide Association Study for Indicator Traits of Sexual Precocity in Nellore Cattle

Whole-genome sequencing provides new insights into genetic mechanisms of tropical adaptation in Nellore (Bos primigenius indicus)

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