Comparative molecular epidemiological investigation on different bovine herpes viruses

Peshev, R.; Christensen, Laurids Siig; Christova, L.

doi:10.1016/s0147-9571(98)00016-2

Cited by 2 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mock-infected calves (group 3: calf Nos. [24][25][26][27][28][29] were included as controls. The inoculum for calves in group 1 (calf Nos.…”

Section: Animal Inoculation and Experimental Designmentioning

confidence: 99%

“…Mock-infected calves (Nos. [24][25][26] were euthanatized on days 6, 10, and 16 (PI and PR, respectively).…”

Section: Animal Inoculation and Experimental Designmentioning

confidence: 99%

“…Nasal and conjunctival swabs were collected daily from animals in groups 1 and from three animals in group 3 (calf Nos. [24][25][26]. For calves in group 2 and three animals in group 3 (calf Nos.…”

Section: Antemortem Sample Collectionmentioning

confidence: 99%

“…In contrast, the occurrence of BHV-5-associated neurologic disease has been only sporadically reported in other parts of the world. 17,26,28,29,32 BHV-1 and BHV-5 are difficult to differentiate by both virologic and serologic methods. Although both viruses are neurotropic, they differ in their ability to cause encephalitis.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Primary Infection, Latency, and Reactivation of Bovine Herpesvirus Type 5 in the Bovine Nervous System

et al. 2002

View full text Add to dashboard Cite

Abstract. Bovine herpesvirus type 5 (BHV-5) infection in calves causes meningoencephalitis, a fatal disease highly prevalent in South America. To study the pathogenesis of BHV-5 infection in cattle, 12 calves (group 1: acute infection) and 11 calves (group 2: latent infection) were intranasally inoculated with an Argentinean BHV-5 isolate at 10 8 and 10 4.7 tissue culture infective doses, respectively; six calves (control group) were mock infected. At 3 months postinoculation, all of the calves in group 2 and three calves in group 3 were given dexamethasone to reactivate the virus. The animals were euthanatized between days 6 and 17 postinoculation (group 1) and between days 6 and 16 postreactivation (group 2). Seventy-five percent and 91% of animals in groups 1 and 2, respectively, excreted BHV-5 in nasal and ocular discharges. Following dexamethasone administration, 45% of calves shed virus in both types of secretions. Spontaneous virus reactivation and shedding was observed in one calf. Neurologic signs consisting of circling, teeth grinding, ptyalism, jaw chomping, tongue protrusion, and apathy were observed in two animals in group 1 and, during the reactivation period, in four animals in group 2. Macroscopic findings consisted of softening of the cerebral tissue, meningeal hemorrhages and swelling, and edema and hemorrhages of prescapular, retropharyngeal and submandibular lymph nodes. Histologic lesions consisted of meningitis, mononuclear perivascular cuffing, neuronophagia, satellitosis, gliosis, hemorrhage, and necrosis and edema. Lesions in anterior cerebral cortex, medulla, and pons were consistently seen in all the animals of group 1. In the acutely infected animals, lesions in the diencephalon appeared at day 10 postinoculation, whereas in the latently infected calves these lesions were observed as early as at day 6 postreactivation. Latently infected animals developed lesions simultaneously in anterior cortex, medulla, pons, and diencephalon, showing a remarkable difference from the acutely infected group. Trigeminal ganglionitis appeared relatively early in animals of both groups (day 7 postinoculation in group 1 and day 8 postreactivation in group 2).

show abstract

“…Mock-infected calves (group 3: calf Nos. [24][25][26][27][28][29] were included as controls. The inoculum for calves in group 1 (calf Nos.…”

Section: Animal Inoculation and Experimental Designmentioning

confidence: 99%

“…Mock-infected calves (Nos. [24][25][26] were euthanatized on days 6, 10, and 16 (PI and PR, respectively).…”

Section: Animal Inoculation and Experimental Designmentioning

confidence: 99%

Section: Antemortem Sample Collectionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Primary Infection, Latency, and Reactivation of Bovine Herpesvirus Type 5 in the Bovine Nervous System

et al. 2002

View full text Add to dashboard Cite

show abstract

“…The PCR is important for rapid diagnosis but because of common clinical features sometimes a recognition of different types of BHV 1 as respiratory, genital or abortogenic is necessary for assessment of the epizootological situation. It is well known that the DNA from BHV 1 isolates, associated with respiratory infections (IBR virus), has different restriction enzyme patterns compared to the DNA isolated from viruses associated with genital infections (IPV virus) [ 11,12 ].…”

mentioning

confidence: 99%

New Molecular Data on Epidemiological Study of Bovine Herpesvirus 1 Strains

Peshev¹,

Christova²

2013

Proceeding BAS

View full text Add to dashboard Cite

Isolated in Bulgaria from animals with different clinical entity, bovine herpes virus 1 (BHV 1) strains are investigatied by molecular biological methods. Based on genome bank data, PCR proving gB, gD, gE and gC genes are developed and adapted and the PCR sensitivity and specificity are determined.The developed PCR is specific for BHV 1 strains and heterologous viruses cannot be multiplied by approved primers. It is determined that PCR technique offers a high resolution capacity compared to the virus isolation test.After investigation by restrictase fragment pattern (RFP) analysis with HPA I and HIND III restriction enzymes (REs), it is found that NIND III RE allows for the distinction of the respiratory from genital and abortogenic BHV 1 strains.The results demonstrate that the proved PCRs identify the BHV 1 strains with a high specificity and resolution and the RFP analysis by HIND III RE allows for the differentiation among investigated BHV 1 virus strains.

show abstract

Comparative molecular epidemiological investigation on different bovine herpes viruses

Cited by 2 publications

References 16 publications

Primary Infection, Latency, and Reactivation of Bovine Herpesvirus Type 5 in the Bovine Nervous System

Primary Infection, Latency, and Reactivation of Bovine Herpesvirus Type 5 in the Bovine Nervous System

New Molecular Data on Epidemiological Study of Bovine Herpesvirus 1 Strains

Contact Info

Product

Resources

About