Comparative microbiological assays of members of the vitamin B complex in yeast and liver extracts

Emery, W. B.; McLeod, Nathan; Robinson, F. A.

doi:10.1042/bj0400426

Cited by 31 publications

(8 citation statements)

References 8 publications

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“…to allow for loss by heat destruction during sterilization. The trace element solution of Emery, McLeod & Robinson (1946), with the addition of cobalt nitrate (0.02 g./l. ), was used to provide trace elements in the defined media.…”

Section: Organismmentioning

confidence: 99%

Cellulose production by Acetobacter acetigenum in defined medium

Dudman¹

1959

Journal of General Microbiology

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S-Y:Growth and cellulose production by Acetobacter acetigenum strain EA-I was limited by nitrogen concentrations below 0.01 yo (w/v) in glucose defined medium. Ammonium sulphate and asparagine+glutamic acid (50% of each)were equivalent as nitrogen sources when compared on a nitrogen weight basis over the range 0.001-0.1 yo-N; at higher concentrations ammonium sulphate decreased growth and cellulose synthesis, while asparagine + glutamic acid became stimulatory. When used as adjuncts in glucose defined medium, acetate, citrate and succinate at suitable concentrations increased growth and cellulose synthesis, by as much as 20-80-fold under the most favourable conditions. Ethanol stimulated growth but did not increase cellulose synthesis. The addition of calcium or m a g nesium carbonate to defined medium inhibited growth. The influence of glucose concentration in defined medium on growth and cellulose synthesis was relatively slight in the absence of succinate; in the presence of 0~084~-succinate the cultures showed a much greater response to glucose concentration, with a maximum cellulose yield that was 7-6 times greater than the maximum in the absence of succinate, and was associated with a 1 -9-fold increase in growth. In hydrolysed molasses medium the addition of succinate decreased growth and cellulose synthesis ; slightly increased cellulose yields were obtained when ethanol and calcium carbonate were added.

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Section: Organismmentioning

confidence: 99%

Cellulose production by Acetobacter acetigenum in defined medium

Dudman¹

1959

Journal of General Microbiology

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“…6H,O, 25 mg., MnSO,.qH,O, I mg., KI, 10 pg., trace elements, 0.1 ml. (Emery, McLeod & Robinson, 1946), calcium-D-pantothenate, 240 pg., thiamine hydrochloride, 200 pg., nicotinic acid, 200 pg., pyridoxine hydrochloride, 250 pg., D-biotin, 0.22 pg., inositol, I mg. The medium was adjusted to pH 5.5.…”

Section: M Power and S W C H A L L I N O R Methodsmentioning

confidence: 99%

The Effects of Inositol-deficiency on the Chemical Composition of the Yeast Cell Wall

Power

Challinor

1969

Journal of General Microbiology

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“…The basal medium used was that described by Cutts & Rainbow (1950 (Emery, McLeod & Robinson, 1946). This strain was independent of exogenous p-AB.…”

Section: Methodsmentioning

confidence: 99%

Non-competitive Sulphanilamide Antagonists for Yeasts

Cutts

Rainbow

1951

Journal of General Microbiology

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SUMMARY: Sulphanilamide inhibition of the growth of certain yeasts and yeastlike organisms was overcome in a non-competitive manner by methionine, adenine and histidine, which are therefore regarded as end-products of enzyme systems for which p-aminobenzoic acid is essential. The methionine system was the most sensitive to sulphanilamide, followed in order by those of adenine and histidine.In the presence of methionine, adenine and histidine together, the test organisms were still sensitive to sulphanilamide, implying that p-aminobenzoic acid is concerned in still another system, or systems. The nature of these systems is obscure, but the inhibition is not overcome by purines, pyrimidines or pteroylglutamic acid, nor by single amino-acids, except in one case when m-cysteine showed some effect. In another case, a low concentration of choline chloride was effective.

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Comparative microbiological assays of members of the vitamin B complex in yeast and liver extracts

Cited by 31 publications

References 8 publications

Cellulose production by Acetobacter acetigenum in defined medium

Cellulose production by Acetobacter acetigenum in defined medium

The Effects of Inositol-deficiency on the Chemical Composition of the Yeast Cell Wall

Non-competitive Sulphanilamide Antagonists for Yeasts

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