Comparative microarray analysis of <i>Arabidopsis thaliana</i> and <i>Arabidopsis halleri</i> roots identifies nicotianamine synthase, a ZIP transporter and other genes as potential metal hyperaccumulation factors

Weber, Michael; Harada, Emiko; Vess, Christoph; Roepenack‐Lahaye, Edda von; Clemens, Stephan

doi:10.1046/j.1365-313x.2003.01960.x

Cited by 398 publications

(378 citation statements)

References 59 publications

(69 reference statements)

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“…AhNAS2 and AhNAS3 can complement Zn-sensitive phenotypes when expressed in yeast [45,47]. Roots of A. halleri were found to contain around 3-fold higher levels of NA than roots of A. thaliana [47]. A similar result has been obtained for T. caerulescens in comparison to T. arvense, albeit after exposure to non-toxic concentrations of Ni [64].…”

Section: The Transport Of Chelators and Metal Chelatessupporting

confidence: 63%

“…Both A. halleri and T. caerulescens highly express NAS2, encoding one isoform of NAS, in their root tissues [46,47]. High expression of NAS genes was also found in the shoots, of NAS3 in A. halleri and of NAS1 and NAS4 in T. caerulescens [45,49].…”

Section: The Transport Of Chelators and Metal Chelatesmentioning

confidence: 97%

“…Note that ZIP9 was only found to be highly expressed in A. halleri roots, and NRAMP1/NRAMP5 only in shoots of T. caerulescens. However, ZIP9 constitutes a main candidate gene for constitutively enhanced Zn uptake in A. halleri roots [44,47], and another member of the NRAMP family, NRAMP3, is highly expressed in A. halleri shoots. One other metal homeostasis gene, NAS2 (At5g56080) encoding a nicotianamine synthase, was found to be highly expressed in roots of both hyperaccumulator species (see Section 4.5).…”

Section: Uptake Into Root Cellsmentioning

confidence: 99%

“…This suggests a divergence of AhNAS2 regulation between shoots and roots, similar to that found for AhZIP9. AhNAS2 and AhNAS3 can complement Zn-sensitive phenotypes when expressed in yeast [45,47]. Roots of A. halleri were found to contain around 3-fold higher levels of NA than roots of A. thaliana [47].…”

Section: The Transport Of Chelators and Metal Chelatesmentioning

confidence: 99%

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Transition metal transport

2007

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Transition metal transporters are of central importance in the plant metal homeostasis network which maintains internal metal concentrations within physiological limits. An overview is given of the functions of known transition metal transporters in the context of the unique chemical properties of their substrates. The modifications of the metal homeostasis network associated with the adaptation to an extreme metalliferous environment are illustrated in two Brassicaceae metal hyperaccumulator model plants based on cross-species transcriptomics studies. In a comparison between higher plants and unicellular algae, hypotheses are generated for evolutionary changes in metal transporter complements associated with the transition to multicellularity.

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Section: The Transport Of Chelators and Metal Chelatessupporting

confidence: 63%

Section: The Transport Of Chelators and Metal Chelatesmentioning

confidence: 97%

Section: Uptake Into Root Cellsmentioning

confidence: 99%

Section: The Transport Of Chelators and Metal Chelatesmentioning

confidence: 99%

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Transition metal transport

2007

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“…Other metal chelators such as organic acids or amino acids, particularly histidine and nicotianamine have also been connected to metal tolerance or hyperaccumulation in plants (Krämer et al 1996;Sarret et al 2002;Vacchina et al 2003;Weber et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Isolation of Zn-responsive genes from two accessions of the hyperaccumulator plant Thlaspi caerulescens

Hassinen

Tervahauta

Halimaa

et al. 2006

Planta

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Several populations with diVerent metal tolerance, uptake and root-to-shoot transport are known for the metal hyperaccumulator plant Thlaspi caerulescens. In this study, genes diVerentially expressed under various Zn exposures were identiWed from the shoots of two T. caerulescens accessions (calaminous and non-calaminous) using Xuorescent diVerential display RT-PCR. cDNA fragments from 16 Zn-responsive genes, including those encoding metallothionein (MT) type 2 and type 3, MRP-like transporter, pectin methylesterase (PME) and Ole e 1-like gene as well as several unknown genes, were eventually isolated. The fulllength MT2 and MT3 sequences diVer from those previously isolated from other Thlaspi accessions, possibly representing new alleles or isoforms. Besides the diVerential expression in Zn exposures, the gene expression was dependent on the accession. Thlaspi homologues of ClpP protease and MRP transporter were induced at high Zn concentrations. MT2 and PME were expressed at higher levels in the calaminous accession. The MTs and MRP transporter expressed in transgenic yeasts were capable of conferring Cu and Cd tolerance, whereas the Ole e 1-like gene enhanced toxicity to these metals. The MTs increased yeast intracellular Cd content. As no signiWcant diVerences were found between Arabidopsis and Thlaspi MTs, they apparently do not diVer in their capacity to bind metals. However, the higher levels of MT2 in the calaminous accession may contribute to the Zn-adapted phenotype.

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Cadmium Detoxification in Plants: Involvement of ABC Transporters

Plaza

Bovet

2008

Trace Elements as Contaminants and Nutrients

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Comparative microarray analysis of Arabidopsis thaliana and Arabidopsis halleri roots identifies nicotianamine synthase, a ZIP transporter and other genes as potential metal hyperaccumulation factors

Cited by 398 publications

References 59 publications

Transition metal transport

Transition metal transport

Isolation of Zn-responsive genes from two accessions of the hyperaccumulator plant Thlaspi caerulescens

Cadmium Detoxification in Plants: Involvement of ABC Transporters

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