Comparative metabolomics analysis on invigorating blood circulation for herb pair Gui-Hong by ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry and pattern recognition approach

Li, Shujiao; Lin, Hang; Tang, Yuping; Li, Weixia; Shen, Juan; Kai, Jun; Yue, Shi‐Jun; Shang, Guanxiong; Zhu, Zhenhua; Shang, Erxin; Zhang, Changbin; Zhang, Li; Yan, Hui; Liu, Pei; Duan, Jin‐Ao

doi:10.1016/j.jpba.2015.01.029

Cited by 26 publications

(29 citation statements)

References 17 publications

(17 reference statements)

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“…Recently, we successfully discovered the underlying blood-activating mechanisms of Danggui-Honghua by metabolomics analysis. Four potential metabolic pathways were speculated, including phenylalanine metabolism, sphingolipid metabolism, arachidonic acid metabolism, and arginine and proline metabolism63. In the present study, arginine and proline metabolism, arachidonic acid metabolism, and sphingolipid signaling pathway (including sphingolipid metabolism) were also deciphered, which were consistent with our previous metabolomics results63.…”

Section: Resultssupporting

confidence: 90%

Herb pair Danggui-Honghua: mechanisms underlying blood stasis syndrome by system pharmacology approach

Yue

Xin

Fan

et al. 2017

Sci Rep

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115

121

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Herb pair Danggui-Honghua has been frequently used for treatment of blood stasis syndrome (BSS) in China, one of the most common clinical pathological syndromes in traditional Chinese medicine (TCM). However, its therapeutic mechanism has not been clearly elucidated. In the present study, a feasible system pharmacology model based on chemical, pharmacokinetic and pharmacological data was developed via network construction approach to clarify the mechanisms of this herb pair. Thirty-one active ingredients of Danggui-Honghua possessing favorable pharmacokinetic profiles and biological activities were selected, interacting with 42 BSS-related targets to provide potential synergistic therapeutic actions. Systematic analysis of the constructed networks revealed that these targets such as HMOX1, NOS2, NOS3, HIF1A and PTGS2 were mainly involved in TNF signaling pathway, HIF-1 signaling pathway, estrogen signaling pathway and neurotrophin signaling pathway. The contribution index of every active ingredient also indicated six compounds, including hydroxysafflor yellow A, safflor yellow A, safflor yellow B, Z-ligustilide, ferulic acid, and Z-butylidenephthalide, as the principal components of this herb pair. These results successfully explained the polypharmcological mechanisms underlying the efficiency of Danggui-Honghua for BSS treatment, and also probed into the potential novel therapeutic strategies for BSS in TCM.

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Section: Resultssupporting

confidence: 90%

Herb pair Danggui-Honghua: mechanisms underlying blood stasis syndrome by system pharmacology approach

Yue

Xin

Fan

et al. 2017

Sci Rep

Self Cite

115

121

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“…In this study, plasma arachidonic acid and PC were significantly increased in BSS rats ( p < 0.01) (Figure ), indicating that the arachidonic acid metabolism was disturbed in the BSS rats. This result is consistent with the report of others (Li et al, ). Compared with the BSS group, pre‐administration of DGPPs significantly reduces plasma arachidonic acid levels ( p < 0.01) (Figure ).…”

Section: Discussionsupporting

confidence: 94%

“…Our experimental result showed that nine differential metabolites (five in plasma and four in urine) were involved in the arginine and proline metabolic pathways (Figure ), indicating that BSS is closely related to the disorder of arginine and proline metabolism. This is consistent with the report of Li et al (). l ‐Arginine is a conditional essential amino acid in humans and rodents, it is a direct precursor for the biosynthesis of nitric oxide (NO, an endothelium‐derived relaxing factor), giving it an important role in blood pressure regulation (Andrew & Mayer, ; Dong et al, ).…”

Section: Discussionsupporting

confidence: 94%

“…Our previous studies showed that the blood‐enriching effect of JDG (Ji et al, ), the anti‐inflammatory effect of volatile oil from JDG or CDG and the hepatoprotective effect of polysaccharides from CDG were significantly enhanced (Hua, Xue, Zhang, Wei, & Ji, ; Zhong et al, ), which may be caused by changes in its chemical composition and content (Ji et al, ). At present, research on the herb pair Gui‐Hong and Danggui‐zhiqiao has shown a significant effect on promoting blood circulation and relieving blood stasis (Li et al, ; Wang, Li, et al, ). However, there is no corresponding research on the effects of DG and its processed products (DGPPs), and their mechanisms are still unclear.…”

Section: Introductionmentioning

confidence: 99%

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Metabolomics study on promoting blood circulation and ameliorating blood stasis: Investigating the mechanism of Angelica sinensis and its processed products

Yuan

Zhong

Hua

et al. 2019

Biomedical Chromatography

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Angelica sinensis (Danggui, DG) parched with alcohol (Jiu Danggui, JDG) and charred DG are the main processed products of DG, which are used to treat blood stasis syndrome (BSS). However, their therapeutic effect and mechanisms are still unclear. Based on an acute rat BSS model, the intervention effects of DG and its processed products (DGPPs) were evaluated by the hemorheology and coagulation function parameters. Meanwhile, plasma and urine metabolites were detected and analyzed by liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry and multivariate statistical analysis method. The results of hemorheology, coagulation function parameters and metabolomics all showed that the BSS model was successfully established, DGPPs intervention could significantly relieve rats BSS and the therapeutic effect of JDG was best. Moreover, 23 differential metabolites (14 in plasma and nine in urine) were identified that were closely related to the BSS, involving seven potential target metabolic pathways. DGPP intervention showed different degrees of reverse effect on these metabolites. JDG was the most effective owing to extensive regulation effect on differential metabolites. This study provides a reference for understanding the pathological mechanism of BSS and the mechanism of DGPPs, which lays a theoretical foundation for the rational use of DGPPs in clinical practice.

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“…THSWD is widely used to treat blood stasis syndrome‐related gynecologic diseases, such as dysmenorrhea, amenorrhea or menoxenia (Zhu, Shang, Duan, Qian, & Tang, ; Li, Yang, et al, ). THSWD can also significantly improve hemorheological and blood coagulation (Li, Lin, et al, ), regulate blood routine index and increase antioxidation and anti‐inflammatory activities (Liu et al, ; Liu, Duan, Bai, & Su, ). In the present study, the effects of THSWD on acute blood stasis syndrome were investigated on the basis of the combined results of the plasma metabolomics study and the findings from the metabolic network.…”

Section: Introductionmentioning

confidence: 99%

Effects of Tao‐Hong‐Si‐Wu decoction on acute blood stasis in rats based on a LC‐Q/TOF‐MS metabolomics and network approach

Hua

et al. 2017

Biomedical Chromatography

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A novel approach using metabolomics coupled with a metabolic network was used to investigate the effects of Tao-Hong-Si-Wu decoction (THSWD) on the rat model of acute blood stasis syndrome. Acute blood stasis syndrome was induced by placing the rats in ice-cold water following two injections with epinephrine. The hemorheological indicators [whole blood viscosity (WBV) and plasma viscosity (PV)] and the blood coagulation indicators [thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen (FIB)] were detected. The nonparametric univariate method and multivariate statistical analysis were performed for determining the potential biomarkers. A correlation map was structured between biochemical indicators and hub metabolites to explain the effects mechanism of THSWD. After the administration of THSWD, the levels of WBV, PV, TT, APTT and FIB returned to levels observed in the control group. According to metabolomics coupled with metabolic network analysis, the intervention of THSWD in rats with acute blood stasis syndrome induced substantial and characteristic changes in their metabolic profiles. Fifteen metabolites were screened, which mainly involved 10 pathways and five hub metabolites, namely, l-glutamate, l-phenylalanine, N-acylsphingosine, arachidonic acid and phosphatidate. The biochemical indicators and hub metabolites could be adjusted to close to normal levels by THSWD. Therefore, combining metabolomics and metabolic network helped to evaluate the effects of THSWD on acute blood stasis.

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Comparative metabolomics analysis on invigorating blood circulation for herb pair Gui-Hong by ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry and pattern recognition approach

Cited by 26 publications

References 17 publications

Herb pair Danggui-Honghua: mechanisms underlying blood stasis syndrome by system pharmacology approach

Herb pair Danggui-Honghua: mechanisms underlying blood stasis syndrome by system pharmacology approach

Metabolomics study on promoting blood circulation and ameliorating blood stasis: Investigating the mechanism of Angelica sinensis and its processed products

Effects of Tao‐Hong‐Si‐Wu decoction on acute blood stasis in rats based on a LC‐Q/TOF‐MS metabolomics and network approach

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