Comparative Metabolomics Analyses of Plantaricin Q7 Production by <i>Lactobacillus plantarum</i> Q7

Bu, Youngmin; Yang, Hui; Li, Jianxun; Liu, Yinxue; Liu, Tongjie; Gong, Pimin; Zhang, Lanwei; Wang, Shumei; Yi, Huaxi

doi:10.1021/acs.jafc.1c03533

Cited by 12 publications

(5 citation statements)

References 33 publications

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“…The function and utilization of L. plantarum have received much attention. At present, more and more bacteriocin-producing L. plantarum have been reported [ 15 , 16 ], but relevant studies were mainly focused on the isolation and purification, antibacterial mechanism and high-efficiency expression of bacteriocins [ 17 , 18 ]. There were few reports on the probiotic functions of bacteriocin-producing L. plantarum .…”

Section: Introductionmentioning

confidence: 99%

Screening and Probiotic Potential Evaluation of Bacteriocin-Producing Lactiplantibacillus plantarum In Vitro

Liu

et al. 2022

Foods

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Probiotics are gaining attention due to their functions of regulating the intestinal barrier and promoting human health. The production of bacteriocins is one of the important factors for probiotics to exert beneficial properties. This study aimed to screen bacteriocin-producing Lactiplantibacillus plantarum and evaluate the probiotic potential in vitro. It was found that L. plantarum Q7, L. plantarum F3-2 and L. plantarum YRL45 could produce bacteriocins and inhibit common intestinal pathogens. These three strains had probiotic potential with tolerance to the gastrointestinal environmental and colonization in the gut, and exhibited various degrees of anti-inflammatory activity and tight junction function in the intestinal barrier. Particularly, L. plantarum YRL45 could significantly (p < 0.05) reduce the increase in nitric oxide (NO), prostaglandin E2 (PGE2), necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS), thereby easing inflammatory response. L. plantarum F3-2 could remarkably (p < 0.05) up-regulate the expression levels of ZO-1, Occludin and Claudin-1 in intestinal epithelial injured cells, which was conducive to protecting the intestinal barrier. These findings provided fundamental information about the probiotic properties of bacteriocin-producing L. plantarum, which suggested that L. plantarum Q7, L. plantarum F3-2 and L. plantarum YRL45 had the potential to be used as novel probiotic strains.

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Section: Introductionmentioning

confidence: 99%

Screening and Probiotic Potential Evaluation of Bacteriocin-Producing Lactiplantibacillus plantarum In Vitro

Liu

et al. 2022

Foods

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“…For instance, L. plantarum could need l-Glu supplementation to metabolise it and enhance the bacteria growth 54 . Likewise, l-Glu could also be required to activate the secondary metabolism to produce postbiotic compounds such as GABA 55 or plantaricin 56 . Furthermore, l-Glu is usually supplemented directly into the fermentation media of L. plantarum strains due to this amino acid is the GABA precursor 57 .…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of gamma-aminobutyric acid by Lactiplantibacillus plantarum K16 as an alternative to revalue agri-food by-products

Diez-Gutiérrez

Vicente

Sáenz

et al. 2022

Sci Rep

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Probiotic metabolites, known as postbiotics, have received attention due to their wide variety of promoting health effects. One of the most exciting postbiotic is gamma-aminobutyric acid (GABA), widely produced by lactic acid bacteria, due to its benefits in health. In addition, the performance of the biosynthesis of GABA by Lactiplantibacillus plantarum could be modulated through the modification of fermentation parameters. Due to their high nutritional value, agri-food by-products could be considered a useful fermentation source for microorganisms. Therefore, these by-products were proposed as fermentation substrates to produce GABA in this study. Previously, several experiments in Man Rogosa Sharpe (MRS) broth were performed to identify the most critical parameters to produce GABA using the strain Lactiplantibacillus plantarum K16. The percentage of inoculum, the initial pH, and the concentration of nutrients, such as monosodium glutamate or glucose, significantly affected the biosynthetic pathway of GABA. The highest GABA yield was obtained with 500 mM of monosodium glutamate and 25 g/L of glucose, and an initial pH of 5.5 and 1.2% inoculum. Furthermore, these investigated parameters were used to evaluate the possibility of using tomato, green pepper, apple, or orange by-products to get GABA-enriched fermented media, which is an excellent way to revalorise them.

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“…Considering the upregulation of related genes and proteins in galactose metabolism, this could because the significant induction that occurs at low concentrations of glutamate and glutamine makes them the most economic strategy for enhancing plantaricin production in L. paraplantarum RX-8. Bu et al (2021b) reported that the plantaricin synthesis of L. plantarum Q7 was promoted by the exogenous addition of glutamate at 16 h, but plantaricin Q7 production was not stimulated by glutamate at 2, 6, and 12 h. Although S. cerevisiae could secrete different kinds of amino acids that are crucial to the survival of L. plantarum, the supplementing CDM35 medium with glutamine, threonine, phenylalanine, tryptophan, and serine, which were under the observed concentrations, had only a minimal impact on L. plantarum growth (Ponomarova et al, 2017). This is because the synergistic nature of multicomponent cross-feeding was challenging to characterize.…”

Section: Inducing E Ects Of Key Metabolites On Plantaricin Productionmentioning

confidence: 99%

“…Bacteriocins are proteins or peptides that display antibacterial activity against various spoilage and pathogenic bacteria (Bu et al, 2021b). Bacteriocins from lactic acid bacteria (LAB) are regarded as safe, natural antimicrobials for food biological preservatives (Bu et al, 2021a).…”

Section: Introductionmentioning

confidence: 99%

Bacteriocin production enhancing mechanism of Lactiplantibacillus paraplantarum RX-8 response to Wickerhamomyces anomalus Y-5 by transcriptomic and proteomic analyses

Nie

Zhu

et al. 2023

Front. Microbiol.

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Plantaricin is a kind of bacteriocin with broad-spectrum antibacterial activity on several food pathogens and spoilage microorganisms, showing potential in biopreservation applications. However, the low yield of plantaricin limits its industrialization. In this study, it was found that the co-culture of Wickerhamomyces anomalus Y-5 and Lactiplantibacillus paraplantarum RX-8 could enhance plantaricin production. To investigate the response of L. paraplantarum RX-8 facing W. anomalus Y-5 and understand the mechanisms activated when increasing plantaricin yield, comparative transcriptomic and proteomic analyses of L. paraplantarum RX-8 were performed in mono-culture and co-culture. The results showed that different genes and proteins in the phosphotransferase system (PTS) were improved and enhanced the uptake of certain sugars; the key enzyme activity in glycolysis was increased with the promotion of energy production; arginine biosynthesis was downregulated to increase glutamate mechanism and then promoted plantaricin yield; and the expression of several genes/proteins related to purine metabolism was downregulated and those related to pyrimidine metabolism was upregulated. Meanwhile, the increase of plantaricin synthesis by upregulation of plnABCDEF cluster expression under co-culture indicated that the PlnA-mediated quorum sensing (QS) system took part in the response mechanism of L. paraplantarum RX-8. However, the absence of AI-2 did not influence the inducing effect on plantaricin production. Mannose, galactose, and glutamate were critical metabolites and significantly simulate plantaricin production (p < 0.05). In summary, the findings provided new insights into the interaction between bacteriocin-inducing and bacteriocin-producing microorganisms, which may serve as a basis for further research into the detailed mechanism.

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Comparative Metabolomics Analyses of Plantaricin Q7 Production by Lactobacillus plantarum Q7

Cited by 12 publications

References 33 publications

Screening and Probiotic Potential Evaluation of Bacteriocin-Producing Lactiplantibacillus plantarum In Vitro

Screening and Probiotic Potential Evaluation of Bacteriocin-Producing Lactiplantibacillus plantarum In Vitro

Biosynthesis of gamma-aminobutyric acid by Lactiplantibacillus plantarum K16 as an alternative to revalue agri-food by-products

Bacteriocin production enhancing mechanism of Lactiplantibacillus paraplantarum RX-8 response to Wickerhamomyces anomalus Y-5 by transcriptomic and proteomic analyses

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