Comparative metabolomic responses to gibberellic acid and 6-benzylaminopurine in Cunila menthoides Benth. (Lamiaceae): a contribution to understand the metabolic pathways

Abstract

Gibberellic acid elicited synthesis of many phenols from different classes and enhanced production of sesquiterpenoids, polyterpenoids, steroids and monoterpenoids compared to control and 6-benzylaminopurine. Little is known about the effects of 6-benzylaminopurine (BA) and gibberellic acid (GA3) on the synthesis of secondary metabolites in species of Lamiaceae. In this study, for the first time, the profile of secondary metabolites in plantlets of Cunila menthoides was characterized, using UPLC-ESI-Qq-oaTOF-MS. Ninety metabolites were identified, including polyphenols and terpenes. BA down-regulated most of the identified molecules in relation to GA3 and MS0 (control). The results showed that GA3 elicited synthesis of many phenols from different classes, and seemed to play a major role in the shikimate pathway in relation to BA. GA3 enhanced production of sesquiterpenoids, polyterpenoids, steroids and monoterpenoids compared to MS0 and BA, and also seemed to positively influence the MEP/DOXP and MVA pathways. These data show the most comprehensive metabolomic profile of Cunila menthoides to date, and the effects of BA and GA3 on the synthesis of secondary metabolites, modulating quantitative aspects of metabolism in Lamiaceae.

The influence of cultivation conditions on the formation of psychoactive salvinorin A, salvinorin B, rosmarinic acid and caffeic acid in Coleus scutellarioides

“…The precursor of salvinorins is geranylgeranyl diphosphate (GGPP), the biosynthesis of which occurs via the 2-C-methyl-erythritol 4-phosphate (MEP) pathway 46 , 47 . 6-benzyloaminopurine reduce the amount of compounds witch are biosynthesized on the MEP pathway 48 , similar as in our case. The other studies indicate the influence of NAA on the MEP pathway 49 , 50 .…”

Metabolomic analysis and ecofriendly enrichment of sunflower meal extract

“…showed that the antioxidant activity of this constituent in sunflower oil was less active compared with caffeic acid. As for the plumerubroside, it was isolated from a water‐soluble extract of the stem bark of Plumeria rubra and also has been found in plantlets of Cunila menthoides and wheat ears at the flowering stage 81‐83 . Therishitin, a phytoalexin associated with plant defense, has been described in different Helianthus species as an antimicrobial compound 84 …”

“…As for the plumerubroside, it was isolated from a water-soluble extract of the stem bark of Plumeria rubra and also has been found in plantlets of Cunila menthoides and wheat ears at the flowering stage. [81][82][83] Therishitin, a phytoalexin associated with plant defense, has been described in different Helianthus species as an antimicrobial compound. 84 Adeleke and Babalola 74 and Guo et al 3 have reported phenolic compounds with distinct biological activities in sunflower seed and by-products.…”