Comparative Metabolomic and Transcriptome Analysis Reveal Distinct Flavonoid Biosynthesis Regulation Between Petals of White and Purple Phalaenopsis amabilis

Meng, Xianghe; Li, Ge; Gu, Lin; Sun, Yu; Li, Zongyun; Wu, Xiaoqing; Dong, Tingting; Zhu, Mingku

doi:10.1007/s00344-019-10025-y

Cited by 35 publications

(28 citation statements)

References 53 publications

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“…NACs are promising candidate factors for genetic engineering to improve crop tolerance, which have been extensively demonstrated by numerous stress-responsive NACs in various plant species (Mohanta et al, 2020). A total of 74 NACs were differentially expressed in the two phenotypes under salt stress, and the transcription of IbNAC7 (one of the 74 NACs) was remarkably induced by salt, cold, ABA, and ACC treatments (Meng et al, 2018), suggesting that IbNAC7 may be involved in the response to environmental cues. Subsequently, salt tolerance test of transgenic Arabidopsis overexpressing IbNAC7 suggested this gene played important roles in salt tolerance.…”

Section: Discussionmentioning

confidence: 96%

“…Previously, 12 stress-responsive IbNAC genes were selected based on the present RNA-seq data. Among them, the transcription of IbNAC7 (one of the 74 NACs) was remarkably upregulated by multiple abiotic stresses and hormones, such as salt, cold, ABA, and ACC (Meng et al, 2018), indicating that IbNAC7 may be involved in the stress response of sweet potato.…”

Section: Identification Of Salt-responsive Tfs In Sweet Potato and Ovmentioning

confidence: 99%

“…Sweet potato (Ipomoea batatas L.) is the only crop with starch storage roots in the Convolvulaceae family (Liu, 2017;Arisha et al, 2020), and is one of the most important food crops, ranking seventh in the world and fourth in China (Meng et al, 2018). Sweet potato is widely applied for human food, animal feed, and for manufacturing starch and alcohol.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome and Proteome Analysis of Salt-Tolerant and Salt-Sensitive Sweet Potato and Overexpression of IbNAC7 Confers Salt Tolerance in Arabidopsis

et al. 2020

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Salt stress is one of the major devastating factors affecting the growth and yield of almost all crops, including the crucial staple food crop sweet potato. To understand their molecular responses to salt stress, comparative transcriptome and proteome analysis of salt-tolerant cultivar Xushu 22 and salt-sensitive cultivar Xushu 32 were investigated. The results showed the two genotypes had distinct differences at the transcription level and translation level even without salt stress, while inconsistent expression between the transcriptome and proteome data was observed. A total of 16,396 differentially expressed genes (DEGs) and 727 differentially expressed proteins (DEPs) were identified. Wherein, 1,764 DEGs and 93 DEPs were specifically expressed in the tolerant genotype. Furthermore, the results revealed that the significantly upregulated genes were mainly related to the regulation of ion accumulation, stress signaling, transcriptional regulation, redox reactions, plant hormone signal transduction, and secondary metabolite accumulation, which may be involved in the response of sweet potato to salt stress and/or may determine the salt tolerance difference between the two genotypes. In addition, 1,618 differentially expressed regulatory genes were identified, including bZIP, bHLH, ERF, MYB, NAC, and WRKY. Strikingly, transgenic Arabidopsis overexpressing IbNAC7 displayed enhanced salt tolerance compared to WT plants, and higher catalase (CAT) activity, chlorophyll and proline contents, and lower malondialdehyde (MDA) content and reactive oxygen species (ROS) accumulation were detected in transgenic plants compared with that of WT under salt stress. Furthermore, RNA-seq and qRT-PCR analysis displayed that the expression of many stress-related genes was upregulated in transgenic plants. Collectively, these findings provide revealing insights into sweet potato molecular response to salt stress and underlie the complex salt tolerance mechanisms between genotypes, and IbNAC7 was shown as a promising candidate gene to enhance salt tolerance of sweet potato.

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Section: Discussionmentioning

confidence: 96%

Section: Identification Of Salt-responsive Tfs In Sweet Potato and Ovmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome and Proteome Analysis of Salt-Tolerant and Salt-Sensitive Sweet Potato and Overexpression of IbNAC7 Confers Salt Tolerance in Arabidopsis

et al. 2020

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“…Phalaenopsis and the materials of this study belong to Orchidaceae. In the metabolomic analysis between petals of white and purple Phalaenopsis, 142 differential flavonoid-related metabolites, including 17 anthocyanins metabolites, were detected by flavonoids-targeted metabolomic analysis [16]. As the upstream reaction of anthocyanin and other flavonoids, Phenylalanine was first converted to P-coumaroyl-CoA under the catalysis of PAL, C4H and 4CL [24].…”

Section: Discussionmentioning

confidence: 99%

“…Flavonoids-targeted metabolomics refers to the analysis of small molecular metabolites of flavonoids (anthocyanins), which is often used to analyze the formation mechanism of plant color. Analysis of Flavonoids-targeted metabolomics in the white and purple flowers of Phalaenopsis identified 142 different flavonoid-related metabolites, of which the most important anthocyanin was the derivative of cyaniding [16]. A study on green and purple asparagus suggested that the difference in color was mainly caused by the contents of peonidin and cyanidin and their glycoside derivatives [17].…”

Section: Introductionmentioning

confidence: 99%

Comparative Metabolomic Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Leaf Color Development of Cymbidium sinense ‘Red Sun’

Gao

Ren

Wei

et al. 2020

IJMS

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The colorful leaf is an important ornamental character of Cymbidium sinense (C. sinense), especially the red leaf, which has always been attracted by breeders and consumers. However, little is documented on the formation mechanism of the red leaf of C. sinense. In this study, the changing patterns of flavonoid-related metabolites, corresponding enzyme activities and genes expression in the leaves of C. sinense ‘Red Sun’ from red to yellow and finally to green was investigated. A total of 196 flavonoid-related metabolites including 11 anthocyanins metabolites were identified using UPLC-MS/MS-based approach. In the process of leaf color change, 42 metabolites were identified as having significantly different contents and the content of 28 differential metabolites turned to zero. In anthocyanin biosynthetic pathway, content of all 15 identified metabolites showed downregulation trend in the process of leaf color change. Among the 15 metabolites, the contents of Naringenin chalcone, Pelargonidin O-acetylhexoside and Anthocyanin 3-O-beta-d-glucoside decreased to zero in the green leaf stage. The changing pattern of enzyme activity of 10 enzymes involved in the anthocyanin biosynthetic pathway showed different trends from red leaves that have turned yellow and finally green, while the expression of genes encoding these enzymes was all down-regulated in the process of leaf color change. The results of this study revealed the types of flavonoid-related metabolites and the comprehensive analysis of metabolites content, enzyme activities and genes expression providing a new reference for breeders to improve the leaf color of C. sinense ‘Red Sun’.

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Widely targeted metabolic analysis revealed the changed pigmentation and bioactive compounds in the ripening Berchemia floribunda (Wall.) Brongn. fruit

Shuai

Liu²,

Liao

et al. 2021

Food Science & Nutrition

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Berchemia plants were important materials for Chinese traditional medicines due to their special secondary metabolites. Unlike the root, stem and leaf tissues, Berchemia floribunda (Wall.) Brongn. fruit was lacked of systematic metabolic investigation. Biochemical analysis found that the total flavonoid and total phenolic content of Berchemia fruit pulp showed a peak value at red ripe stage, and then decreased, but the total anthocyanin content sharply increased along with the coloration. By widely targeted metabolomic analysis, 644 metabolites were identified and categorized into 23 groups mainly including flavonoid, organic acids, amino acids, lipids, phenylpropanoid, nucleotides, alkaloids, carbohydrates, alcohols, anthocyanins & proanthocyanidins, vitamins, terpenes, polyphenols, phenolamides, quinones, indole derivatives, and sterides. Among them, 111 metabolites and 123 metabolites respectively showed up‐ and down‐regulation from break stage to full mature. KEGG enrichment analysis indicated that active secondary metabolism such as biosynthesis of phenylpropanoids, flavonoid, and alkaloids happened during Berchemia fruit ripening. More importantly, Cyanidin‐3‐O‐galactoside and other 3 cyanidins were found to be the predominant pigments in mature Berchemia fruit and increased cyanidins and pelargonidins but decreased anthocyanins might be contributed to the purple pigmentation of Berchemia fruit. Interestingly, 29 pharmaceutical compounds previously reported in other Berchemia tissues were also detected in ripening Berchemia fruit pulp: 8 flavonoid, 2 quinones & sucrose showed up‐regulated accumulation while 6 polyphenols, 5 flavonoid, 3 phenylpropanoid, 2 organic acids, 1 quinones and β‐sitosterol showed down‐regulated accumulation In conclusion, our first comprehensive metabolic fingerprint will promote the further study of B. floribunda fruit and its medical and food application.

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Comparative Metabolomic and Transcriptome Analysis Reveal Distinct Flavonoid Biosynthesis Regulation Between Petals of White and Purple Phalaenopsis amabilis

Cited by 35 publications

References 53 publications

Comparative Transcriptome and Proteome Analysis of Salt-Tolerant and Salt-Sensitive Sweet Potato and Overexpression of IbNAC7 Confers Salt Tolerance in Arabidopsis

Comparative Transcriptome and Proteome Analysis of Salt-Tolerant and Salt-Sensitive Sweet Potato and Overexpression of IbNAC7 Confers Salt Tolerance in Arabidopsis

Comparative Metabolomic Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Leaf Color Development of Cymbidium sinense ‘Red Sun’

Widely targeted metabolic analysis revealed the changed pigmentation and bioactive compounds in the ripening Berchemia floribunda (Wall.) Brongn. fruit

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