Comparative Metabolism and Structure of BCKD-E2 in Primary Biliary Cirrhosis

Recently, crystalized mouse ketimine reductase/CRYM complexed with NADPH was found to have pyruvate bound in its active site. We demonstrate that the enzyme binds α-keto acids, such as pyruvate, in solution, and catalyzes the formation of N-alkyl-amino acids from alkylamines and α-keto acids (via reduction of imine intermediates), but at concentrations of these compounds not expected to be encountered in vivo. These findings confirm that, mechanistically, ketimine reductase/CRYM acts as a classical imine reductase and may explain the finding of bound pyruvate in the crystallized protein.

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Determination of selected α-keto acids in dried blood samples using HPLC with fluorescence detection

Kanďár

Kopčil

Laštovičková

2022

Journal of Pharmaceutical and Biomedical Analysis

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Comparative Metabolism and Structure of BCKD-E2 in Primary Biliary Cirrhosis

Cited by 7 publications

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The autoantibodies of primary biliary cirrhosis: clinico pathological correlations

The autoantibodies of primary biliary cirrhosis: clinico pathological correlations

Ketimine reductase/CRYM catalyzes reductive alkylamination of α-keto acids, confirming its function as an imine reductase

Determination of selected α-keto acids in dried blood samples using HPLC with fluorescence detection

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