Comparative measurement of equine influenza virus antibodies in horse sera by single radial hemolysis, neutralization, and hemagglutination inhibition tests

Yamagishi, H; Nakagawa, Takashi; Shimoda, K.; Ide, Seiya; Igarashi, Y; Yoshioka, Isao; Matumoto, Minoru

doi:10.1128/jcm.15.4.660-662.1982

Cited by 12 publications

(13 citation statements)

References 2 publications

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“…11,14,28 The correlation between SRH and MN assays observed in this study is in agreement with the findings of the previous studies that used equine influenza strains and H3N2 influenza strain. 11,29,30 However, unlike Wang et al, 30 who found much higher correlations between SRH and MN assays than between SRH-HI and HI-MN assays, we found no significant differences among them. The correlation among the three assays was also evaluated for influenza B strains (Victoria and Yamagata lineages), showing good agreement.…”

Section: F I G U R E 1 Continuedcontrasting

confidence: 99%

“…In addition, the assay agreement on protection, as defined by 11,29,30,33 Previous studies involving A strains support the strong agreement between HI and SRH assays found in present study. 11,14,28 The correlation between SRH and MN assays observed in this study is in agreement with the findings of the previous studies that used equine influenza strains and H3N2 influenza strain.…”

Section: F I G U R E 1 Continuedsupporting

confidence: 90%

“…Overall, the HI, SRH, and MN assays have shown significant positive correlations, especially for influenza A strains. 11,14,[27][28][29] A recent study has revealed that the correlation between SRH and MN is greater than that between SRH-HI and HI-MN assays. 30 ELISA shows good agreement between the HI and MN assays; notably, ELISA seems to be more sensitive than HI assay, especially in detecting low levels of antibody, owing to the low background.…”

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confidence: 99%

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Comparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses

Trombetta

Remarque

Mortier

et al. 2018

Influenza Resp Viruses

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BackgroundThe immunological response to influenza vaccine and/or natural infection is evaluated by serological techniques, the most common being hemagglutination inhibition (HI), single radial hemolysis (SRH), and virus neutralization assays, which is commonly used in a micro‐neutralization (MN) format. ELISA is not officially required; however, this assay is able to measure different class‐specific antibodies. The four assays identify different sets or subsets of antibodies.ObjectivesThe aim of this study was to establish the correlation among four serological assays using four seasonal influenza strains.MethodsThe HI, SRH, MN assays, and ELISA were performed on four seasonal influenza strains.ResultsA strong positive correlation was found between HI and MN and between SRH and MN assays for influenza A strains. The B strains also showed good correlations among the three assays. A positive correlation was also found between ELISA and the “classical” assays for all strains. Concerning the correlates of protection, as defined by HI ≥ 40 and SRH ≥ 25 mm2, good agreement was observed for the influenza A strains. By contrast, the agreement for the B strains was very low.ConclusionsThere is a positive strong correlation among the four serological assays for both A and B strains, especially for the HI and MN assays. There is good agreement on correlates of protection between HI and SRH assays for the A strains, but very low agreement for the B strains, suggesting higher sensitivity of SRH than HI assay in detecting antibodies against the influenza B viruses.

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Section: F I G U R E 1 Continuedcontrasting

confidence: 99%

Section: F I G U R E 1 Continuedsupporting

confidence: 90%

mentioning

confidence: 99%

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Comparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses

Trombetta

Remarque

Mortier

et al. 2018

Influenza Resp Viruses

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“…We found the SRH assay to be less sensitive than the MN assay but more sensitive than HAI, as 16% of participants had SRH titres below 11,12 suggest that SRH may be more sensitive for detecting antibodies against influenza B than influenza A viruses, while the study by Yamagishi et al 10 suggests that the sensitivity of SRH test is lower than that of HAI, at least for equine influenza virus.…”

Section: Discussionmentioning

confidence: 63%

Single radial haemolysis compared to haemagglutinin inhibition and microneutralization as a correlate of protection against influenza A H3N2 in children and adolescents

Wang

Russell

Brewer

et al. 2017

Influenza Resp Viruses

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BackgroundSerum antibodies are often used as correlates of protection for influenza. Three commonly used serological assays for detecting influenza‐specific serum antibodies are single radial haemolysis (SRH), haemagglutinin inhibition (HAI) and microneutralization (MN). However, here are limited data on SRH as well as HAI and MN as correlates of protection against influenza in children and adolescents. There are also limited data that compare SRH to HAI and MN.ObjectivesWe sought primarily to understand how SRH titres correlate to protection against influenza infection in children and adolescents. We also compare SRH to HAI and MN.MethodsOf 732 healthy Hutterite children and adolescents aged between 3 and 15 years were enrolled from Saskatchewan and Alberta, Canada, in the 2008‐2009 flu season. Blood samples were drawn from participants at baseline and between 3 and 5 weeks post‐vaccination. Serum antibodies against seasonal H3N2 influenza were measured by SRH, HAI and MN assays.ResultsThe estimates of protective efficacy fluctuated when the cut‐off SRH values increased. The correlation between HAI and SRH titres was 0.53 (P<.01); between MN and SRH 0.82 (P<.01); and between HAI and MN 0.50 (P<.01). Sixteen per cent of participants had SRH titres below the detection limit, compared to 7% and 34% for the MN and HAI assays.Conclusions SRH had the worst correlation with protection against seasonal H3N2 in children and adolescents compared to MN and HAI. SRH, HAI and MN titres were significantly correlated with each other. SRH was less sensitive than MN but more sensitive than HAI.

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“…Thus, SRH is the serological test of choice for measuring vaccine efficacy. Few studies, however, have compared EI serological techniques during outbreaks [ 46 , 47 ]. Similar to the findings by Morley et al [ 46 ], a good correlation between the HI and SRH test was observed in this study, although SRH demonstrated greater diagnostic sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Multifocal Equine Influenza Outbreak with Vaccination Breakdown in Thoroughbred Racehorses

et al. 2018

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Equine influenza (EI) outbreaks occurred on 19 premises in Ireland during 2014. Disease affected thoroughbred (TB) and non-TB horses/ponies on a variety of premises including four racing yards. Initial clinical signs presented on 16 premises within a two-month period. Extensive field investigations were undertaken, and the diagnostic effectiveness of a TaqMan RT-PCR assay was demonstrated in regularly-vaccinated and sub-clinically-affected horses. Epidemiological data and repeat clinical samples were collected from 305 horses, of which 40% were reported as clinically affected, 39% were identified as confirmed cases and 11% were sub-clinically affected. Multivariable analysis demonstrated a significant association between clinical signs and age, vaccination status and number of vaccine doses received. Vaccine breakdown was identified in 31% of horses with up to date vaccination records. This included 27 horses in four different racing yards. Genetic and antigenic analysis identified causal viruses as belonging to Clade 2 of the Florida sublineage (FCL2). At the time of this study, no commercially available EI vaccine in Ireland had been updated in line with World Organisation for Animal Health (OIE) recommendations to include a FCL2 virus. The findings of this study highlight the potential ease with which EI can spread among partially immune equine populations.

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Comparative measurement of equine influenza virus antibodies in horse sera by single radial hemolysis, neutralization, and hemagglutination inhibition tests

Cited by 12 publications

References 2 publications

Comparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses

Comparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses

Single radial haemolysis compared to haemagglutinin inhibition and microneutralization as a correlate of protection against influenza A H3N2 in children and adolescents

Multifocal Equine Influenza Outbreak with Vaccination Breakdown in Thoroughbred Racehorses

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