Comparative mapping of the two wheat leaf rust resistance loci &lt;i&gt;Lr1&lt;/i&gt; and &lt;i&gt;Lr10&lt;/i&gt; in rice and barley

Gallego, Francesca; Feuillet, Catherine; Messmer, Monika; Penger, Anja; Graner, Andreas; Yano, Masahiro; Sasaki, Takuji; Keller, Beat

doi:10.1139/gen-41-3-328

Cited by 8 publications

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Wheat

Varshney¹,

Balyan²,

Langridge³

Cereals and Millets

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AFLP maps Wheat DHs (Garnet × Saunders) 426-Penner et al. (1998) Wheat ITMI RILs (W7984 × Opata85) 140-Hazen et al. (2002) Composite maps Aegilops tauschii F2s [Ae. tauschii var meyeri (TA1691) × 732-Boyko et al. 2002 Ae. tauschii var typical (TA1704)] Wheat-einkorn F2s (T. monococcum × T. boeoticum 81-Kojima et al. (1998) ssp. boeoticum) (RFLPs, RAPDs, ISSRs) Wheat-einkorn F2s/ F3s (T. monococcum ssp. 335 714 Dubcovsky et al. (1996) monococcum DV92 × T. monococcum ssp. (mainly RFLPs) Aegilopoides C3116) Wheat-durum RILs [T. durum (Messapia) × 88 2,063 (total) Lotti et al. (2000) T. turgidium (MG4343)] (AFLPs, RFLPs) Wheat-durum F2s (T. dicoccoides acc. Hermon H52 × 545 3,169-3,180 Peng et al. (2000b) T. durum cultivar Langdon (Ldn) (AFLPs, RAPDs, SSRs) Wheat-durum RILs (Jennah Khetifa × Cham1) 306 3,598 Nachit et al. (2001) (RFLPs, SSRs , AFLPs) Wheat-durum RILs (Omrabi5 × T.dioccoides 600545 × 279 2,289 Elouafi and Nachit (2004) Ombrabi 5) (RFLP, SSR, SSP) Wheat-emmer RILs 549 Nevo (2001) (SSRs, AFLPs, RAPDs) Wheat DHs (Schomburgk × Yarralinka) 147-Parker et al. (1998) (RFLPs, SSRs, AFLPs) Wheat RILs (T. aestivum L. var. Forno × 230 2,469 Messmer et al. (1999) T. spelta L. var. Oberkulmer) (RFLPs, SSRs) Wheat DHs (Cranbook × Halbred, CD87 × 355 to 902-Chalmers et al. (2001) Katepwa, Sunco × Tasman) (RFLPs, SSRs, AFLPs) Wheat DHs (Courtot × Chinese Spring) 380 2,900 Sourdille et al. (2000b) (RFLP, SSRs, AFLPs) Wheat DHs (Courtot × Chinese Spring) 659 3,685 Sourdille et al. (2003) (RFLP, SSRs, AFLPs) Wheat F5s (Arina × Forno) 396 3,086 Paillard et al. (2003) (RFLPs, SSRs) Wheat DHs (Beaver x Soissons) 241 2,290 Verma et al. (2004) (AFLPs, SSRs) a Details and updated version of these maps are available at GrainGenes (http://wheat.pw.usda.gov/GG2/maps.shtml)

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Wheat

Varshney¹,

Balyan²,

Langridge³

Cereals and Millets

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The gibberellic-acid insensitive dwarfing gene sdw3 of barley is located on chromosome 2HS in a region that shows high colinearity with rice chromosome 7L

et al. 2004

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In this study, comparative high resolution genetic mapping of the GA-insensitive dwarfing gene sdw3 of barley revealed highly conserved macrosynteny of the target region on barley chromosome 2HS with rice chromosome 7L. A rice contig covering the sdw3-orthologous region was identified and subsequently exploited for marker saturation of the target interval in barley. This was achieved by (1) mapping of rice markers from the orthologous region of the rice genetic map, (2) mapping of rice ESTs that had been physically localized on the rice contig, or (3) mapping of barley ESTs that show strong sequence similarity to coding sequences present in the rice contig. Finally, the sdw3 gene was mapped to an interval of 0.55 cM in barley, corresponding to a physical distance of about 252 kb in rice, after employing orthologous EST-derived rice markers. Three putative ORFs were identified in this interval in rice, which exhibited significant sequence similarity to known signal regulator genes from different species. These ORFs can serve as starting points for the map-based isolation of the sdw3 gene from barley.

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EST derived SSR markers for comparative mapping in wheat and rice

et al. 2004

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Structural and functional relationships between the genomes of hexaploid wheat ( Triticum aestivum L.) (2n=6x=42) and rice (Oryza sativa L.) (2n=2x=24) were evaluated using linkage maps supplemented with simple sequence repeat (SSR) loci obtained from publicly available expressed sequence tags (ESTs). EST-SSR markers were developed using two main strategies to design primers for each gene: (1) primer design for multiple species based on supercluster analysis, and (2) species-specific primer design. Amplification was more consistent using the species-specific primer design for each gene. Forty-four percent of the primers designed specifically for wheat sequences were successful in amplifying DNA from both species. Existing genetic linkage maps were enhanced for the wheat and rice genomes using orthologous loci amplified with 58 EST-SSR markers obtained from both wheat and rice ESTs. The PCR-based anchor loci identified by these EST-SSR markers support previous patterns of conservation between wheat and rice genomes; however, there was a high frequency of interrupted colinearity. In addition, multiple loci amplified by these primers made the comparative analysis more difficult. Enhanced comparative maps of wheat and rice provide a useful tool for interpreting and transferring molecular, genetic, and breeding information between these two important species. These EST-SSR markers are particularly useful for constructing comparative framework maps for different species, because they amplify closely related genes to provide anchor points across species.

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Comparative mapping of the two wheat leaf rust resistance loci <i>Lr1</i> and <i>Lr10</i> in rice and barley

Cited by 8 publications

References 0 publications

Wheat

Wheat

The gibberellic-acid insensitive dwarfing gene sdw3 of barley is located on chromosome 2HS in a region that shows high colinearity with rice chromosome 7L

EST derived SSR markers for comparative mapping in wheat and rice

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