Comparative mapping of the fragile histidine triad (FHIT) gene in cattle, river buffalo, sheep and goat by FISH and assignment to BTA22 by RH‐mapping: a comparison with HSA3

Abstract

Common fragile sites can be damaged by exposure to a variety of carcinogens. The fragile histidine triad (FHIT) gene, including the most active human chromosomal fragile site (FRA3B) at chromosome band HSA3p14.2,1 has been proposed as a tumour suppressor gene for a variety of tumours.2 The most common response to carcinogen exposure is deletions at the FHIT locus that alter the gene structure and function. In this study we assign the FHIT gene in cattle, river buffalo, sheep and goat chromosomes by comparative fluorescence in situ hybridization (FISH)-mapping. In addition, the assignment to BTA22 was confirmed by typing the marker across a bovine radiation hybrid (RH) panel

Comparative genomic mapping of the bovine Fragile Histidine Triad (FHIT) tumour suppressor gene: characterization of a 2 Mb BAC contig covering the locus, complete annotation of the gene, analysis of cDNA and of physiological expression profiles

“…Second, BACs bI0643H09 and bI0947B04, boxed in Figure [see Additional file 2 ], could be directly linked in the contig, as they were both positive to the intron 8–1 tag. Third, the sequence of the BAC-ends, available for clones bI0195D05, bI0520A05, and bI0545B04, was BLASTed to the bovine genome database and identified a contig assigned to BTA11 [Genbank: NW_988236 ], which is extraneous to the FHIT bovine locus, previously mapped to BTA22 [ 10 ].…”

“…In a previous report, we mapped the FHIT gene by FISH and radiation hybrids using a bovine EST that matched human exon 9 [ 10 ]. Here we designed primers on the human FHIT cDNA to cross-amplify the bovine cDNA by means of RT-PCR.…”

“…A bovine BAC containing exon 9 of the gene was already available [ 10 ], and was used as a starting point to isolate adjacent clones from a BAC library [ 12 ]. In addition, the human FHIT genomic sequence [Build 36.1; Genbank: NC_000003 .1], nt 59710076 to 61212124 was used as a source of STSs.…”

“…In a recent study we isolated an exon tag for bovine FHIT which allowed us to map the gene by FISH to BTA22q24 and with radiation hybrids to the telomeric region of BTA22 [ 10 ]. Here we used a comparative genomic approach with human FHIT to completely annotate the bovine gene.…”

Novel insights into the genetic basis of buffalo reproductive performance

“…Two available buffalo genome sequences on the NCBI platform are not displayed on chromosomes and genes are not annotated (UMD CASPUR WB 2.0; https://www.ncbi.nlm.nih.gov/assembly/GCF_000471725.1/ ). Cattle and buffalo have been reported to be closely related, sharing homology in chromosome banding and gene mapping [ 49 , 50 ]. It has also been confirmed that the bovine genome is a useful source of markers for buffalo genome mapping [ 51 , 52 ], three available buffalo GWAS were constructed using the cattle genome reference [ 4 , 18 , 53 ].…”