Comparative mammalian cell cytotoxicity of wastewater with elevated bromide and iodide after chlorination, chloramination, or ozonation

Dong, Shengkun; Nguyen, Thanh H.; Plewa, Michael J.

doi:10.1016/j.jes.2017.03.030

Cited by 29 publications

(20 citation statements)

References 28 publications

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“…As numerous human epidemiological studies have already demonstrated the correlation between DBP exposure and several human health impacts, including bladder cancer, birth defects, and fetal loss, − CHO cells that have been frequently used in DBP toxicity study were selected in the present study to comparatively assess the in vitro toxicity of bromophenols. CHO-K1 cell line acquired from the American Type Culture Collection was used in the present study to comparatively evaluate the cytotoxicity of 4-BrPh and 2,4-DiBrPh. This assay measures the reduction in cell density on flat-bottom 96-well microplates as a function of DBP concentration over a period of 72 h (three cell cycles) compared with untreated controls.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive Insights into the Interactions of Two Emerging Bromophenolic DBPs with Human Serum Albumin by Multispectroscopy and Molecular Docking

Zhang

Yang

et al. 2019

ACS Omega

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Disinfection byproducts (DBPs) are of high concern due to their ubiquitous existence in disinfected drinking water and their potential adverse effects on human health. In this study, two bromophenolic DBPs 4-bromophenol (4-BrPh) and 2,4-dibromophenol (2,4-DiBrPh) were selected to investigate their binding interactions with human serum albumin (HSA) using spectroscopic techniques and a molecular docking method. The experimental results demonstrated that both of the DBPs could bind with HSA to form bromophenol–HSA complexes with the HSA secondary structure being changed, primarily relying on hydrogen bonding and van der Waals forces, but 2,4-DiBrPh showed a higher binding affinity. The binding constants of 4-BrPh–HSA and 2,4-DiBrPh–HSA were 2.66 × 103 and 1.83 × 104 M–1 at 310 K, respectively. Molecular docking results revealed the locations of the binding sites for bromophenols on HSA (locating in subdomain IB). In addition, the comparative toxicity of the two bromophenolic DBPs was evaluated with the mammalian cytotoxicity bioassay and the results showed that the LC50 values of 4-BrPh and 2,4-DiBrPh were 3.08 × 10–5 and 1.09 × 10–5 M, respectively. Notably, 2,4-DiBrPh with a higher binding affinity toward HSA also showed a significantly higher toxic potency than 4-BrPh.

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Section: Methodsmentioning

confidence: 99%

Comprehensive Insights into the Interactions of Two Emerging Bromophenolic DBPs with Human Serum Albumin by Multispectroscopy and Molecular Docking

Zhang

Yang

et al. 2019

ACS Omega

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“…These in vitro quantitative bioassays generate estrogen and toxicity metrics across treatment strategies to define processes that may reduce potential health risks from reclaimed wastewaters. 10 A similar approach was used to evaluate the toxicity of DBPs in waters. 8,11−17 We reported the comparative toxicity of waters prior to and post DPR treatments at Army installations.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Integration of analytical biology to quantify negative impacts in eukaryotic cell cultures enables direct comparisons between the existing and alternative water sources. These in vitro quantitative bioassays generate estrogen and toxicity metrics across treatment strategies to define processes that may reduce potential health risks from reclaimed wastewaters . A similar approach was used to evaluate the toxicity of DBPs in waters. ,− We reported the comparative toxicity of waters prior to and post DPR treatments at Army installations. , …”

Section: Introductionmentioning

confidence: 99%

Comparison of Estrogenic, Spectroscopic, and Toxicological Analyses of Pilot-Scale Water, Wastewaters, and Processed Wastewaters at Select Military Installations

Dong

Page

Hur

et al. 2021

Environ. Sci. Technol.

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Reuse of water requires the removal of contaminants to ensure human health. We report the relative estrogenic activity (REA) of reuse treatment design scenarios for water, wastewaters, and processed wastewaters before and after pilot-scale treatment systems tested at select military facilities. The comparative relationships between REA, several composite toxicological endpoints, and spectroscopic indicators were evaluated for different reuse treatment trains. Four treatment processes including conventional and advanced treatments reduced the estrogenicity by at least 33%. Biologically based methods reduced estrogenicity to below detection levels. Conventional treatment scenarios led to significantly less reduction of adverse biological endpoints compared to the advanced treatment scenarios. Incorporating the anaerobic membrane bioreactor reduced more endpoints with higher reduction percentages compared to the sequencing batch reactor design. Membrane technology and advanced oxidation generated reductions across all biological endpoints, from 65% (genotoxicity) to 100% (estrogenicity). The design scenarios featuring a low-cutoff mechanical screen filter, intermittent activated carbon biofilter, and membrane filtration achieved the highest percent reduction and produced water with the lowest negative biological endpoints. Spectroscopic indicators demonstrated case-specific relationships with estrogenicity and toxicity. Estrogenicity consistently correlated with cytotoxicity and thiol reactivity, indicating the potential for preliminary estrogenicity screening using thiol reactivity.

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“…15,18−22,24−34 Recent studies showed that chloraminated water with elevated bromide and iodide levels produces water that is more cytotoxic and genotoxic than chlorinated water, because of the enhanced formation of iodinated DBPs. 21,35 Previous studies reported that oil and gas wastewater discharged to surface waters after partial treatment leads to elevated bromide and iodide concentrations in receiving streams and at downstream drinking water plants 1,3,13 and enhanced formation of brominated and iodinated DBPs upon disinfection. DBPs reported to date from oil and gas wastewater impacts include bromo-and iodo-THMs, bromo-HAAs, bromo-acetaldehydes, bromo-nitromethanes, and bromo-acetonitriles.…”

Section: ■ Introductionmentioning

confidence: 99%

“…To reduce regulated DBP levels, many drinking water plants have switched from chlorine to monochloramine for disinfection. While monochloramine reduces regulated trihalomethanes (THMs) and haloacetic acids (HAAs), it promotes the formation of more toxic unregulated DBPs, including iodinated and nitrogenous DBPs. ,− ,− Recent studies showed that chloraminated water with elevated bromide and iodide levels produces water that is more cytotoxic and genotoxic than chlorinated water, because of the enhanced formation of iodinated DBPs. , …”

Section: Introductionmentioning

confidence: 99%

Identification and Comparative Mammalian Cell Cytotoxicity of New Iodo-Phenolic Disinfection Byproducts in Chloraminated Oil and Gas Wastewaters

Liberatore

Plewa

Wagner

et al. 2017

Environ. Sci. Technol. Lett.

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Hydraulic fracturing wastewaters discharged to surface water have led to elevated bromide and iodide levels, as well as enhanced formation of brominated trihalomethanes, haloacetic acids, haloacetonitriles, and iodo-trihalomethanes at downstream drinking water treatment plants, in chlorinated effluent from wastewater treatment plants, and in controlled laboratory studies. This enhanced formation of brominated and iodinated disinfection byproducts (DBPs) raises concerns regarding human health, because they are much more toxic than chlorinated DBPs. This study represents the first nontarget, comprehensive analysis of iodinated DBPs formed in chloraminated produced waters associated with hydraulic fracturing of shale and conventional gas formations. Fifty-six iodo-phenolics were identified, comprising three homologous series of mono-, di-, and tri-iodinated phenols, along with two new classes of DBPs: iodomethylphenols and iododimethylphenols. Four iodo-phenolics (2-iodophenol, 4-iodophenol, 2,4,6-triiodophenol, and 4-iodo-2-methylphenol) were investigated for mammalian cell cytotoxicity. All were cytotoxic, especially 2,4,6-triiodophenol, which was more cytotoxic than all trihalomethanes and most haloacetic acids. In addition, geogenic organic compounds present in the oil and gas produced waters, including methylphenol and dimethylphenol, were found to be potential precursors to these iodo-DBPs.

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Comparative mammalian cell cytotoxicity of wastewater with elevated bromide and iodide after chlorination, chloramination, or ozonation

Cited by 29 publications

References 28 publications

Comprehensive Insights into the Interactions of Two Emerging Bromophenolic DBPs with Human Serum Albumin by Multispectroscopy and Molecular Docking

Comprehensive Insights into the Interactions of Two Emerging Bromophenolic DBPs with Human Serum Albumin by Multispectroscopy and Molecular Docking

Comparison of Estrogenic, Spectroscopic, and Toxicological Analyses of Pilot-Scale Water, Wastewaters, and Processed Wastewaters at Select Military Installations

Identification and Comparative Mammalian Cell Cytotoxicity of New Iodo-Phenolic Disinfection Byproducts in Chloraminated Oil and Gas Wastewaters

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