Comparative lipid content ofCandida lipolytica grown on glucose and onn-hexadecane

Nyns, Edmond‐Jacques; Chiang, Ni-Na; Wiaux, A. L.

doi:10.1007/bf02046431

Cited by 21 publications

(12 citation statements)

References 10 publications

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“…Like other oleaginous yeasts, it can grow on sugars, such as glucose [2], [3], and on hydrophobic substrates (HS) [4]. It can also synthesize and store lipids [5]. In addition, Y. lipolytica is highly tractable genetically, making it a good model species for biotechnological applications, particularly for single-cell oil production [6], [7], [8].…”

Section: Introductionmentioning

confidence: 99%

Comparative Physiology of Oleaginous Species from the Yarrowia Clade

et al. 2013

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Yarrowia lipolytica is a genetically tractable yeast species that has become an attractive model for analyses of lipid metabolism, due to its oleaginous nature. We investigated the regulation and evolution of lipid metabolism in non-Saccharomycetaceae yeasts, by carrying out a comparative physiological analysis of eight species recently assigned to the Yarrowia clade: Candida alimentaria, Y. deformans, C. galli, C. hispaniensis, C. hollandica, C. oslonensis, C. phangngensis and Y. yakushimensis. We compared the abilities of type strains of these species to grow on 31 non hydrophobic (sugars and other carbohydrate compounds) and 13 hydrophobic (triglycerides, alkanes and free fatty acids) carbon sources. Limited phenotypic diversity was observed in terms of the range of substrates used and, in the case of short-chain fatty acids, their toxicity. We assessed the oleaginous nature of these species, by evaluating their ability to store and to synthesize lipids. The mean lipid content of cells grown on oleic acid differed considerably between species, ranging from 30% of cell dry weight in C. oslonensis to 67% in C. hispaniensis. Lipid synthesis in cells grown on glucose resulted in the accumulation of C18:1 (n-9) as the major compound in most species, except for C. alimentaria and Y. yakushimensis, which accumulated principally C18:2(n-6), and C. hispaniensis, which accumulated both C16:0 and C18:1(n-9). Thus, all species of the clade were oleaginous, but they presented specific patterns of growth, lipid synthesis and storage, and therefore constitute good models for the comparative analysis of lipid metabolism in this basal yeast clade.

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Section: Introductionmentioning

confidence: 99%

Comparative Physiology of Oleaginous Species from the Yarrowia Clade

et al. 2013

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“…Neutral lipids were also decreased in hexadecane-grown cells. Candida lipolytica (25) and C. petrophilum (22) contained more lipid when grown on alkanes than when grown on glucose, and the lipid content of Biakeslea trispora increased when alkanes were added to a glucose medium (34). It is evident that the total cell lipid and total phospholipid of C. resinae are influenced by the carbon source, and different n-alkanes have different effects.…”

Section: Chemicalsmentioning

confidence: 99%

Phospholipids of Cladosporium resinae cultured on glucose and on n-alkanes

Kan¹,

Cooney²

1975

Can. J. Microbiol.

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Cladosporium resinae was grown on glucose, on n-dodecane, and on n-hexadecane. Total lipid was greatest in dodecane-grown cells and least in hexadecane-grown cells, while glucose-grown cells contained the most phospholipid and hexadecane-grown cells contained the least. Cells from all three media contained phosphatidylethanolamine and phosphatidylcholine as their major phospholipids, with lesser amounts of phosphatidylserine and traces of a cardiolipin-like compound. The major fatty acids associated with each phospholipid were palmitic acid and one or more 18-carbon unsaturated fatty acids. There was no correlation between n-alkane growth substrate and fatty acyl components of cellular phospholipids.

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“…Hence, numerous reports have appeared describing qualitative and quantitative aspects of cellular lipids in microorganisms grown on n-alkane in an attempt t o obtain the specific metabolic relationship between the alkane substrates and lipid products (DAVIS 1964, DUNLAP and PERRY 1967, NYNS et al 1968, MISHINA et al 1973, HUG and BIECHTER 1973, PATRIK and DUGAN 1974.…”

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confidence: 98%

Cellular fatty acids derived from normal alkanes by Candida rugosa

Iida

Kobayashi

1980

J of Basic Microbiology

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In an attempt to examine the specific metabolic relationship between the alkane substrates and lipid products the pattern of formation of cellular lipid, especially fatty acids, has been studied in the coursu of incubation of cells of C. rugosa with n-alkanes in mineral medium. cis:^, Ci8:i, Ci6:i and Ci6:o fatty acids were the major products formed, irrespective of the odd or even chain length of the alkane used as substrate. These unsaturated fatty acids reached a maximum in the stationary phase. In the case of cells grown on odd-chain n-alkanes (from C,, t o C,,), the proportion of odd-chain cellular fatty acids was markedly high, reaching 77 -88y0 in the n-pent& decane and heptadecane-grown cells. The resulting acids are then metabolized by 8-oxidation or inserted directly or after elongation with C,-units into the cellular fatty acids. The total lipid content of n-alkane (n-C,o-C!20) grown cells in the stationary phase was 16.3-19.0y0 of the dry cell weight, which was about three times as much as that of glucose-grown cells. The chain length of alkane substrates had a significant effect on the lipid content. Ergosterol production from n-hexadecane was twice higher than that from glucose.Fatty acids and fatty acid esters are the most significant direct products of n-alkane metabolism in microorganisms. Hence, numerous reports have appeared describing qualitative and quantitative aspects of cellular lipids in microorganisms grown on n-alkane in an attempt t o obtain the specific metabolic relationship between the alkane substrates and lipid products (DAVIS 1964, DUNLAP and PERRY 1967, NYNS et al. 1968, MISHINA et al. 1973, HUG and BIECHTER 1973, PATRIK and DUGAN 1974.Quality and quantity of cellular fatty acids formed from n-alkanes, however, are greatly influenced by the type of organism and alkane substrate. Since 1956, we have studied the microbial assimilation mechanism of hydrocarbons. We have described the initial oxidation of decane by Candida rugosa JFlOl (IIDA and IIZUKA 1971). I n 1974 we reported the biosynthesis of extracellular monosaccharides from hydrocarbon and the structure of cell wall mannan of n-alkane-grown cells (IIDA and FINNERTY 1974a, b). More recently, we reported on the microbodiea of the hydrocarbon-assimilating yeast, C. rugosa (TANAKA and IIDA 1977).This investigation details the nature and diversity of fatty acids derived from C. rugosa growing a t the expense of a homologous series of odd and even n-alkanes as sole carbon and energy source. Materials and methodsCultivation: The organism used was Candida rugosa JFlO1, which was grown in an inorganic salt medium (pH 7.2) (IIDA and FINNERTY 1974a) containing 1% (v/v) hydrocarbon or 1% (w/v) glucose and sodium acetate. The incubation was carried out a t 27 "C in a 500 ml conical flask containing 100 ml medium on a rotary shaker (220 rpm). The 12 straight-chain n-alkanes used were 99% pure and purchased from Tokyo Chemical Co., Ltd.Assay of cellular fatty acids: The whole cells were collected a t the exponential phase and the Statio...

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Comparative lipid content ofCandida lipolytica grown on glucose and onn-hexadecane

Cited by 21 publications

References 10 publications

Comparative Physiology of Oleaginous Species from the Yarrowia Clade

Comparative Physiology of Oleaginous Species from the Yarrowia Clade

Phospholipids of Cladosporium resinae cultured on glucose and on n-alkanes

Cellular fatty acids derived from normal alkanes by Candida rugosa

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