Comparative karyomorphological analyses of in vitro and in vivo grown plants of Aloe vera L. BURM. f.

Das, Ashok Kumar; Mukherjee, Priyanka; Ghorai, Animesh; Jha, Timir Baran

doi:10.1007/s13237-010-0001-1

Cited by 15 publications

(19 citation statements)

References 23 publications

(24 reference statements)

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“…According to Stebbins (1971) karyotype asymmetry of A. vera is 3B type. These fi ndings support earlier observations of chromosome constitution for A. vera (Sapre 1978;Das et al 2010; Gunjan and Roy 2010).…”

Section: Karyomorphology Of Mitotic Metaphase Chromosomessupporting

confidence: 92%

“…Yet, its importance is partly associated with general interest in plant reproduction and fertility and partly with greater understanding of genome constitution and its exploitation. In contrast to spontaneous mitotic chromosomal aberrations (Vig 1968;Sapre 1975), somatic chromosome characteristics (Sapre 1978;Umesh and Ranganath 2003;Das et al 2010), including the fl uorescent metaphase chromosomes (Alam and Khanam 2005) reported earlier throwing light on the structure and behaviour of mitotic chromosomes, meiotic behaviour especially of prophase I chromosomes is inadequate. The present study aims at providing a comprehensive account of the structure and behaviour of meiotic as well as mitotic chromosomes of A. vera.…”

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confidence: 99%

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Meiotic chromosome behaviour and karyomorphology of Aloe vera (L.) Burm. f.

Chaudhari

Chaudhary

2012

Chromosome Botany

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Aloe vera (L.) Burm. f. (the family Aloaceae) indigenous to Africa is an admired medicinal plant used as a folk medicine. Meiotic division was investigated in A. vera with a view to decipher the behavior of chromosomes during prophase I and the subsequent stages of meiosis following acetocarmine squash technique. The various meiotic stages from leptotene to pollen development were studied. Seven bivalents were clearly visible at diplotene, and the chiasma frequency averaged to 1.86 per bivalent and 13.1 per pollen mother cell (PMC). Prophase I stages are diffi cult to identify due to clumping of chromosome threads. Second nuclear division, however, allows clear distinction of chromosome confi guration. Besides clumping, various other types of chromosomal aberrations such as stickiness and fragments at metaphase I, precocious movement of chromosomes, bridges, laggards and unequal separation of chromosomes at anaphase I and unequal separation of chromosomes at telophase II were noted. Karyomorphological study on the other hand, was carried out in anther wall (mitotic) cells of A. vera. Mitotic as well as meiotic studies in the species showed 2n=14 chromosomes including two pairs of sat-chromosomes. The karyotype of the species was adjudged to be typically asymmetric.

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Section: Karyomorphology Of Mitotic Metaphase Chromosomessupporting

confidence: 92%

mentioning

confidence: 99%

Meiotic chromosome behaviour and karyomorphology of Aloe vera (L.) Burm. f.

Chaudhari

Chaudhary

2012

Chromosome Botany

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“…Finally, a photoplate karyogram of each species was prepared with the help of Adobe Photoshop version 7.0 (Ghosh and Datta 2006). Karyotype details of the two selected Aloe species have been worked out (Das et al 2010) and presented in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Cytomorphological Studies of <i>Aloe variegata</i> L. and <i>Aloe zebrina</i> Baker (Xanthorrhoeaceae) by an Image Analyzing System

Parai¹,

Mukherjee

2014

CYTOLOGIA

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SummaryThe present paper deals with the detailed cytomorphological study of two Aloe species, viz., Aloe variegata L. and A. zebrina Baker, by studying both root tip mitosis as well as pollen mitosis. It was clear from our cytomorphological studies that these two Aloe species reflect the bimodal, asymmetrical karyotype retained within other members of the family Xanthorrhoeaceae. Although both the species were morphologically very similar and had the same diploid chromosome number, 2n=2x=14(8 L+6S), they could be differentiated by quantitative parameters of their karyotypes. It was found that both Aloe species contained the diploid (2n=14) somatic chromosome numbers with one pair of both satellite and secondary constrictions. The total haploid chromosome length in Aloe variegata and A. zebrina was noted to be 3168.49 μm and 3193.49 μm, respectively, and the total forma percentage (TF%) was recorded as 17.99 and 17.42, respectively, which reflects the asymmetric nature of the karyotype. The chromosome number (2n=2x=14) analyzed in root tip mitosis reflecting the diploid karyotype of the two species was also correlated to the number noted in pollen mitosis (n=x=7). These data add to the information about karyosystematics and could support inter-specific relationships.

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“…Actualmente se reportan 400 especies de Aloe (IASC, 2008), de las cuales A. barbadensis y A. arborescens var. natalensis Berger, son las únicas especies cultivadas comercialmente (Das et al, 2010).…”

Section: Introductionunclassified

“…Early last century, products like leaf powder, gel and juice had a high demand from the medical and cosmetic industry of the United States and Europe, which encouraged the establishment of plantations in Mexico, Texas, Dominican Republic, Haiti and Namibia (Añez and Vásquez, 2005). Currently 400 species of Aloe are reported (IASC, 2008), of which A. barbadensis and A. arborescens var natalensis Berger, are the only species commercially cultivated (Das et al, 2010).…”

Section: Introductionmentioning

confidence: 99%