Comparative Investigations on In Vitro Serum Stability of Polymeric Micelle Formulations

Miller, Tobias; Rachel, Reinhard; Besheer, Ahmed; Uezguen, Senta; Weigandt, Markus; Goepferich, Achim

doi:10.1007/s11095-011-0555-x

Cited by 62 publications

(49 citation statements)

References 34 publications

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“…Present challenges are low drug loading capacity, reduced tumour targetability, short in vivo stability and premature drug release [129][130][131][132][133]. The future of these systems implies novelties in their design, such as cross-linking by reversible bonds with stimuli-responsive futures at the site of action [134] and the inclusion of targeting moieties which could enhance cellular uptake, in particular diseased cells through receptor-mediated endocytosis.…”

Section: Drug Deliverymentioning

confidence: 99%

Peptide-Based Polymer Therapeutics

2014

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Abstract:Polypeptides are envisaged to achieve a major impact on a number of different relevant areas such as biomedicine and biotechnology. Acquired knowledge and the increasing interest on amino acids, peptides and proteins is establishing a large panel of these biopolymers whose physical, chemical and biological properties are ruled by their controlled sequences and composition. Polymer therapeutics has helped to establish these polypeptide-based constructs as polymeric nanomedicines for different applications, such as disease treatment and diagnostics. Herein, we provide an overview of the advantages of these systems and the main methodologies for their synthesis, highlighting the different polypeptide architectures and the current research towards clinical applications.

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Section: Drug Deliverymentioning

confidence: 99%

Peptide-Based Polymer Therapeutics

2014

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“…[21][22][23][24][25][26] The combination of AF4-MALS-QELS was previously used to characterize several morphologies of polymer aggregates, in particular micelles. [27][28][29][30] The use of this technique to characterize the shapes of different polymer vesicles has been overlooked so far. Till et.…”

Section: Introductionmentioning

confidence: 99%

Shape characterization of polymersome morphologies via light scattering techniques

Abdelmohsen¹,

Rikken

Christianen

et al. 2016

Polymer

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Polymersomes, vesicles self-assembled from amphiphilic block copolymers, are well known for their robustness and for their broad applicability. Generating polymersomes of different shape is a topic of recent attention, specifically in the field of biomedical applications. To obtain information about their exact shape, tomography based on cryo-electron microscopy is usually the most preferred technique. Unfortunately, this technique is rather time consuming and expensive. Here we demonstrate an alternative analytical approach for the characterization of differently shaped polymersomes such as spheres, prolates and discs via the combination of multi-angle light scattering (MALS) and quasi-elastic light scattering (QELS). The use of these coupled techniques allowed for accurate determination of both the radius of gyration (R g ) and the hydrodynamic radius (R h ). This afforded us to determine the shape ratio ρ (R g /R h ) with which we were able to distinguish between polymersome spheres, discs and rods.

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“…The stability of different kinds of polymeric micelles (composed of polyethylene glycol (PEG), poly(lactic acid) and Tween 80) was evaluated in human serum, using fluorescence-based approach (Forster Resonance Energy Transfer). Asymmetrical Flow Field Flow Fractionation (AF4) was used for the separation of the micelles (further characterized by light scattering) [106]. In other interesting work, Lee et al [84] evaluated chitosan nanostructures integrity after GI tract passage and when in contact with a Caco-2 cell monolayer.…”

Section: Separation Purification and Characterization Of Bio-based Nmentioning

confidence: 99%

Edible Bio-Based Nanostructures: Delivery, Absorption and Potential Toxicity

et al. 2015

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The development of bio-based nanostructures as nanocarriers of bioactive compounds to specific body sites has been presented as a hot topic in food, pharmaceutical and nanotechnology fields. Food and pharmaceutical industries seek to explore the huge potential of these nanostructures, once they can be entirely composed of biocompatible and non-toxic materials. At the same time, they allow the incorporation of lipophilic and hydrophilic bioactive compounds protecting them against degradation, maintaining its active and functional performance. Nevertheless, the physicochemical properties of such structures (e.g., size and charge) could change significantly their behavior in the gastrointestinal (GI) tract. The main challenges in the development of these nanostructures are the proper characterization and understanding of the processes occurring at their surface, when in contact with living systems. This is crucial to 2 understand their delivery and absorption behavior as well as to recognize potential toxicological effects. This review will provide an insight into the recent innovations and challenges in the field of delivery via GI tract using bio-based nanostructures. Also, an overview of the approaches followed to ensure an effective deliver (e.g., avoiding physiological barriers) and to enhance stability and absorptive intestinal uptake of bioactive compounds will be provided. Information about nanostructures' potential toxicity and a concise description of the in vitro and in vivo toxicity studies will also be given.

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Comparative Investigations on In Vitro Serum Stability of Polymeric Micelle Formulations

Abstract: This FRET-based assay is a valuable tool in evaluating and screening serum stability of polymeric micelles and revealed low serum stability of PEG-PVPy micelles compared to polyester-based micelles.

Cited by 62 publications

References 34 publications

Peptide-Based Polymer Therapeutics

Peptide-Based Polymer Therapeutics

Shape characterization of polymersome morphologies via light scattering techniques

Edible Bio-Based Nanostructures: Delivery, Absorption and Potential Toxicity

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